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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 335 results for: dre

Showing: 61 - 80 of 335 results

  2. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...the exact materials that they think they are. To address this, several years ago we partnered with Seqwell...Unfortunately, the standard AAV QC regimen does not address this.  Previous studies have demonstrated that ...hgtb.2019.110 Pacouret S, Bouzelha M, Shelke R, Andres-Mateos E, Xiao R, Maurer A, Mevel M, Turunen H,...

  3. RUBY-Red Siliques

    Type
    Blog Post
    ...Figure 1: An adult plant expressing RUBY. Photos by Andrew Willoughby   Using RUBY as a selection marker...is slightly darker or slightly brown. Photos by Andrew Willoughby The ease of observing RUBY compared...RUBY-red: You can’t beet it!) About the Author  Andrew Willoughby is a graduate student studying plant...

  4. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...Despite all the talk, not much was being done to address the issue head on. It was at this pivotal moment...Meanwhile, Addgene was also thinking about ways to address irreproducibility and thanks to Teytelman’s connection...and best practices could be an actionable way to address the widespread issue of irreproducibility. Use...

  5. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...’s okay! Our blog writer, Beth Kenkel, once had dreams of getting her PhD, but after two years in graduate...true at all. Racheal Komuhendo, a Lab Technician, dreamed of becoming a doctor. After taking a year break... and being around the scientists. I have never dreaded coming to work because I truly enjoy the field ...

  7. Tips for Technical Support Calls

    Type
    Blog Post
    ...I’ve answered hundreds of phone calls and thousands of customer service emails in my six years as a senior...Having spent that long in customer service, I've daydreamed about the ideal customer service interaction ...and it’s wrong. Can you help us fix it? Please address as soon as possible!” and I always begin my responses...

  9. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...sequencing files, either one at a time or as a batch of hundreds, and indicate the guide RNA sequence you used.... at a time, and batch analysis, which compares hundreds of samples simultaneously.  The current version...not guiding CRISPR researchers, she's either day-dreaming about exploring sci-fi worlds or exploring nature...

  10. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...and stalled projects. You want to identify and address these types of harmful conflict early, because ...constructive) conflict. As you perform the experiments to address a reviewer’s concerns, you are making your hypothesis... something else.") The majority of conflicts I address in my current role revolve around missed tasks,...

  11. Five Popular Model Organisms

    Type
    Blog Post
    ...of RNAi in the worm actually rewarded depositors Andrew Fire and Craig Mello a Nobel Prize in 2006. One...mechanotransduction to name a few. For example, Andrew Leifer’s lab utilized the worm to study calcium... 71-94. PubMed Central PMCID: PMC1213120. Fire, Andrew, et al. "Potent and specific genetic interference...

  12. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...low-abundance targets or natively isolated complexes. To address these limitations, the Funabiki Lab developed a... imaging, making it easy to identify dozens or hundreds of particles surrounding each of the highly-visible...80–100 bp, PASSIGE can integrate cargoes over a hundred times that size (demonstrated up to 10.5 kb) by...

  14. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...magnified detail of the box in C. Image adapted from Andrews et al., 2019 under CC-BY 4.0 license.   Selecting...the rat brain sample shown in Figure 2A above, Andrews et al. used the following combination of antibodies... imaging! References and Resources References Andrews, N. P., Boeckman, J. X., Manning, C. F., Nguyen...

  15. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ... effects and variable silencing efficiency. To address these shortcomings, both short hpRNAs (shRNAs) ...downstream characterization. To simultaneously address both limitations of the traditional RNAi approach... K, Bürckstümmer T, Bantscheff M, Steinmetz LM, Drewes G, Huber W (2019) Biological plasticity rescues...

  16. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ... two or three days before sampling (Uphoff and Drexler, 2011). Culturing on agar plates/broth is considered...mycoplasma species. J Microbiol .44: 42–49  Uphoff Cc, Drexler Hg (2002) Comparative Antibiotic Eradication of...-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting Mycoplasma Contamination in...

  17. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...with a partner. WPI’s emphasis on teamwork was what drew me to the school in the first place. The famous ...environmentally friendly as possible. We worked with Andrew, the team’s founder, to create a mission statement...even a lot about everything. Fast forward a few hundred years and there are currently about 5.5 million...

  18. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...postdoc mentor and lab. These topics have been addressed in numerous essays and workshops (see the Careers...give talks or posters about their research. They dread giving a departmental seminar or a joint lab meeting...strategies to rest and re-energize yourself, and to address all of the other parts of your life, which, inconveniently...

  19. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...QC, Canada 45.5017°N 73.5673°W – Vanessa Sung Hundreds of scientists and supporters in Montreal took ...march ended that I loved - there were booths for children to learn little things about science. We need ...to be the perfect opportunity to cement in my children's heads the importance of science and positively...

  20. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... the fight against cancer. Sipuleucel-T by the Dendreon Corporation is currently the only FDA-approved...25998715. PubMed Central PMCID: PMC4491443. 5. Scott, Andrew M., Jedd D. Wolchok, and Lloyd J. Old. "Antibody...receptors for acute lymphoblastic leukaemia in children and young adults: a phase 1 dose-escalation trial...
Showing: 61 - 80 of 335 results