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We narrowed to 296 results for: dre

Showing: 1 - 20 of 296 results

  1. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...important, factors to consider when seeking your dream lab. The principal investigator's point of view... for future success by finding and joining your dream lab, watch the webinar "How to Choose Your Next ...Director, Joanne Kamens.   The goal: Finding your dream lab While none of us will feel completely happy ... joining your dream lab...

  2. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...pass right through our tissues unimpeded. Andrew and Maria dreamed about the possibilities of a magnetoresponsive...tool for applications that don’t even exist yet? Andrew York, Maria Ingaramo, and their team at Calico ...generally appears to be weak — if they exist at all. Andrew and Maria were encouraged by previous reports that...point for developing useful tools. A lucky start As Andrew and Maria explained, “to our surprise and delight...after the magnet was removed (Figure 1). At first, Andrew and Maria were skeptical. The team was careful ...obvious signs of improved response. Then, just when Andrew was about to suggest she deprioritize the project...field (Figure 2); they named this variant MagLOV. Andrew was thrilled to see that MagLOV’s “astonishing”...

  4. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...near-complete excision of the stop sequence. In contrast, Dre recombinase, the close relative of Cre, displayed...recombinase with flies heterozygous for tubulin-GAL4. For Dre, Flp, KD, R, B2 and B3, approximately 50% of offspring...

  5. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo...requested samples of AAV for testing purposes. To address this, we recently released our serotype testing...

  6. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...used in chemogenetics are DREADDs and PSAMs. Using DREADDs DREADDs DREADDS, or designer receptors exclusively... Other DREADDs, such as the KORD receptor, increase this threshold and silence neurons. DREADD-activated...function (Saloman, JL, et al., 2016).   DREADD ligands Several DREADDs, including hM3D(Gq), have been derived...cellular responses. The cellular response set off by a DREADD depends on what G-protein and, consequently, what...what pathway the receptor acts through. The DREADD hM3D(Gq), for example, lowers the threshold for neuronal...popular agonist for muscarinic receptor derived DREADDs has been clozapine-N-oxide (CNO). However, concerns...been raised over the reliability of using CNO as a DREADD agonist due to its ability to be metabolized into...

  7. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...Exclusively Activated by Designer Drugs (DREADDs) (Roth, 2016). DREADDs were engineered from members of the...inhibition). AAV preps from DREADD plasmids allow you to easily express a DREADD receptor in a specific neuronal...This article was written by Alyssa Cecchetelli and Andrew Hempstead. What do smell, taste and sight have ...Tools for Studying GPCR signaling  So with the hundreds of GPCRs out there, how can scientists decipher...receptors- angiotensin II type 1 receptor and the β2 adrenoceptor. GPCRs in chemogenetics  While there’s still...molecule ligands such as clozapine-N-oxide (CNO). DREADDs are ideal for chemogenetics as they are relatively...no constitutive activity. There are an array of DREADDs that activate different G proteins, work in different...

  8. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...isotype-matched control antibody. This control addresses situations where a primary antibody not only binds...   Figure 3: A preimmune serum control addresses situations where a primary antibody not only binds...groups, such as aldehydes in fixed samples. To address this, block positively charged groups with generally... species-on-species staining can be partially addressed with stringent staining protocols and the use ...of noise will determine whether it needs to be addressed. In order to correctly interpret your results,...interferes with data interpretation and can be addressed with a label control. In a label control, the ...to more than their target primary antibody. To address this, include a control wherein each primary antibody...

  9. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    Published
    May 11, 2021, 1:15 p.m.
    ...because the DREADD receptors need to be located on the plasma membrane to be activated by the DREADD actuator...Receptor Exclusively Activated by Designer Drugs (DREADDs) (Armbruster et al., 2007).  We initially used ...to the hM4Di might improve the transport of this DREADD to the plasma membrane. The haemagglutinin (HA)...interfered with the transport to the membrane of the DREADDs. In studies where the fluorescence is not needed...proteins. For example, we saw that another type of DREADD, hM3Dq, is successfully expressed at the plasma... Smith Y (2019) Ultrastructural localization of DREADD s in monkeys. Eur J Neurosci 50:2801–2813 . https...

  10. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...Abhi Aggarwal.   Brandán Pedre Pérez: Redox biosensors Brandán Pedre Pérez is a postdoc at the German...Congratulations to the five winners! Abhi Aggarwal Brandán Pedre Pérez Elizabeth Hubbard Haoyi Qiu He Tian Every... have used it to detect glutamate release from hundreds of synapses at once. “This work has only been ...during oxidative stress. To monitor this activity, Pedre Pérez’s biosensor fuses MPST to roGFP2. Because ...the processes that happen inside the cell,” says Pedre Pérez. Figure 2: Micrographs of the biosensor...

  11. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...new. Chapter 2, “The Professional Scientist,” addresses the challenges of succeeding in science if you...value here is seeing them all in one place. Can we address problems in scientific publishing without acknowledging...Chapter 11)? Even if some of the problems are addressed in a somewhat cursory manner, their inclusion ...genuinely shocking. Mainstream media outlets have addressed CRISPR frequently over the last several years,...over 1,000 authors. The chapter on deep learning addresses the dilemma of creating a technology that could... sections on optogenetics and CRISPR of course address some of the ethical implications of using these...

  12. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...FDA-approved drugs. See our inventory of DREADDs / Chemogenetics AAVs New DREADDs Plasmid Serotype Name 75033...Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST DREADDs / Chemogenetics AAV We have also expanded our inventory...activating and inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab are especially useful for...primates, where fusion fluorescent proteins can affect DREADD trafficking and localization. The Sternson lab ...

  13. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...cloning techniques and troubleshooting methodologies drew me to learn more about the process that was used...driven by two CMV promoters (orange). Figure from Andrews, et. al., Elife  A limitation of hybridoma ...recombinant antibodies in adult rat brain. Figure from Andrews, et. al, Elife. Detailed figure legend here.   ...recombinant antibody plasmid collection References Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold... Andrew Hempstead...

  14. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...83896 AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell-5 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 ...SARS-CoV-2 proteins ChR2 for targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral...plasmid encoding a Cre dependent version of the DREADD hM3D is now also offered as AAV1 (along with AAV...AAV2, AAV5, AAV8, and AAV9). Once activated, this DREADD acts through the Gq pathway and promotes neuronal...

  15. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...consists almost entirely of viruses expressing DREADDs from Bryan Roth’s lab at the University of North...floxed human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control of the ... cre-dependent, meaning that once injected, the DREADD will only be expressed in cells that express cre...further specifies neuron-specific expression of the DREADD (Kügler et al., 2003), which can then be exclusively...mice, rats, or non-human primates. To activate the DREADD, CNO is delivered either through drinking water...

  16. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...innovation will address a variety of challenges and impact the field - its encouraged to dream big! It is...technologies should offer in order to build upon and address some, if not all, of the limitations of current...sets the stage for why your funded proposal will address these limitations and lead to new milestones in... the project and ii) how you, the expert, will address the research challenge. Now is the time to make...

  17. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...yours counts. Dress professionally but still like yourself. It’s better to be overdressed rather than underdressed... underdressed, but read emails from HR carefully because sometimes companies have relaxed dress codes ...interviews. You can also ask HR for clarification on the dress code for the interview. Greet each interviewer and...

  18. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...signaling. For more information on DREADDs, please see the Roth lab's DREADD users blog. New CRISPR plasmids...alleles off or on enables this method to rapidly address questions of tissue-specificity and cell autonomy...bacteria are time-consuming and involve many steps. The Drew Endy and Keith Shearwin labs have developed a new...al., ACS Synth Biol 2013 Sep 20;2(9):537-41. DREADD-based chemogenetic technologies After several...Receptors Exclusively Activated by Designer Drugs (DREADD) plasmids on his own, UNC-Chapel Hill's Bryan Roth...each recombines independently, generating tens or hundreds of possible combinations (depending on the number...

  19. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...but proceed with caution! Loading controls only address one type of error that can impact your western ...red) influence these relationships and must be addressed with separate controls (green). Created with BioRender.com... for different loading controls So how can you address these errors without making incorrect assumptions...blotting quantification” (Gilda & Gomes, 2013). To address this source of error, it’s important to determine...Resources and References References Aldridge, G. M., Podrebarac, D. M., Greenough, W. T., & Weiler, I. J. (2008...

  20. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...regarding the order will be sent to the contact email addresses provided. How do I place an order using a PO number...organization in this field. Please also enter the billing address for your organization. We will contact you directly...facility, we will send an invoice to the billing address that you listed during checkout. This invoice will...additional questions about our ordering process not addressed in this article, check out our Help Center article...
Showing: 1 - 20 of 296 results