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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 336 results for: dre

Showing: 1 - 20 of 336 results

  1. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...and Other Site-Specific Recombinases Cre Plasmids Dre Plasmids Flp Plasmids Other Recombinase Plasmids ...respectively. Other tyrosine recombinases include Dre, R, Nigri, SCre, VCre, and Vika. These systems work...browse highlighted plasmids expressing Cre , Flp , Dre , other recombinases , as well as useful empty backbones...example, Cre with lox sites, Flp with FRT sites, Dre with rox sites), enabling precise genetic manipulation... bp) lox2272, lox5171, lox511, loxN, lox66/lox71 Dre rox TAACTTTAAATAATgccaATTATTTAAAGTTA 14 bp inverted...GAAGTTCCTATTCtctagaaaGTATAGGAACTTC Table 1. Summary of Cre, Dre, and Flp Recognition Sites. Controlling Recombinase...your favorite site-specific recombinase (Cre, Flp, Dre, other recombinases). Use the search bars or sort...

  2. Recombinases AAV Preps

    Type
    Collection
    ...none 8 Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA Syn none...Viral Vector Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV...-iCreV EF1a none 1, PHPeB Zeng 140136 pAAV-EF1a-iDreV EF1a none 1, PHPeB Zeng 140137 pAAV-EF1a-iFlpV EF1a...

  3. AAV Viral Preps

    Type
    Collection
    ...Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate Sensors, Voltage...Packaging Service overview. Function Chemogenetics DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins...

  4. Retrograde AAV viral preps

    Type
    Collection
    ...Recombinases Engel , Nectow 50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression Recombinases Zeng 51669 AAV phSyn1...Green Red Switch Other Recombinases Cre Flp VCre Dre Calcium Sensors GCaMP8f GCaMP8s GCaMP8m GCaMP7f GCaMP7s...Flp-dependent DREADD Gether 83896 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 hDlx Inhibitor DREADD Fishell 83897...Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent DREADD Roth 50474...mCherry Syn Activator DREADD Roth 50475 pAAV-hSyn-hM4D(Gi)-mCherry Syn Inhibitor DREADD Roth 154867 pAAV-hSyn-fDIO-hM4D...83897 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 hDlx Activator DREADD Fishell 98747 pAAV-FLEX-EGFPL10a EF1a ...Optogenetics Activators Inhibitors Bidirectional DREADDs Activators Inhibitors Other Molecular Tools Clear...

  5. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...important, factors to consider when seeking your dream lab. The principal investigator's point of view... for future success by finding and joining your dream lab, watch the webinar "How to Choose Your Next ...Director, Joanne Kamens.   The goal: Finding your dream lab While none of us will feel completely happy ... joining your dream lab...

  6. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...pass right through our tissues unimpeded. Andrew and Maria dreamed about the possibilities of a magnetoresponsive...tool for applications that don’t even exist yet? Andrew York, Maria Ingaramo, and their team at Calico ...generally appears to be weak — if they exist at all. Andrew and Maria were encouraged by previous reports that...point for developing useful tools. A lucky start As Andrew and Maria explained, “to our surprise and delight...after the magnet was removed (Figure 1). At first, Andrew and Maria were skeptical. The team was careful ...obvious signs of improved response. Then, just when Andrew was about to suggest she deprioritize the project...field (Figure 2); they named this variant MagLOV. Andrew was thrilled to see that MagLOV’s “astonishing”...

  8. Deisseroth INTRSECT Collection

    Type
    Collection
    ...pAAV-EF1a-sCre None No 55633 pAAV-EF1a-mCherry-IRES-Dre None No 183535 pAAV-CaMKIIa-Flpo None No Single recombinase-dependent...EYFP Flp Yes 55640 pAAV-Ef1a-dDIO hChR2(H134R)-EYFP Dre No 55639 pAAV-Ef1a-fDIO hChR2(H134R)-EYFP Flp Yes...pAAV-nEF-Coff/Fon DREADD Gi-mCherry Flp AND NOT Cre No 177672 pAAV-nEF-Con/Fon DREADD Gi-mCherry Cre AND...pAAV-nEF-Con/Foff DREADD Gi-mCherry Cre AND NOT Flp Yes 183532 pAAV-nEF Con/Fon DREADD Gq-mCherry Cre AND...pAAV-nEF Con/Foff DREADD Gq-mCherry Cre AND NOT Flp No 183534 pAAV-nEF Coff/Fon DREADD Gq-mCherry Flp AND...Flp AND NOT Cre Yes Dual recombinase-dependent: DREADDs Addgene ID Plasmid Logic Sites and Mutations In-Stock...

  10. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo...requested samples of AAV for testing purposes. To address this, we recently released our serotype testing...

  11. Pipetting Protocol

    Type
    Protocol
    ...first red digit is thousands of µL, the middle is hundreds, and the third is tens. Therefore, 1000 µL would...µL would read as 065. P200: The first digit is hundreds of µL, the second is tens, and the third is ones...digit is tenths, and the third (red) digit is hundredths. Therefore, 1 µL would read as 100 (as shown ...

  12. Gibson Assembly Protocol

    Type
    Protocol
    ...Enzymatic assembly of DNA molecules up to several hundred kilobases. Nature Methods , 6(5), 343–345. https...

  14. CRISPR Library Amplification

    Type
    Protocol
    ...intended use. Typically yields range from several hundred micrograms to a milligram of total plasmid DNA....

  15. Brain Initiative Collection

    Type
    Collection
    ...Gordon Fishell 83896-AAV1 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression...Gordon Fishell 83896-AAV9 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression...Gordon Fishell 83896-AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression...Gordon Fishell 83897-AAV1 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression...Gordon Fishell 83897-AAV9 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression...Gordon Fishell 83897-AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression...pAAV-EF1a-iDreV can be used to generate AAV virus that will expresslight-inducible site-specific iDreV recombinase...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Frataxin FXN Friedreich ataxia Ronald Kahn 11443 pBS human Frataxin (myc tag) FXN Myc T7 Friedreich ataxia ...Frataxin FXN Friedreich ataxia Ronald Kahn 11504 pBabe hygro human Frataxin FXN Friedreich ataxia Ronald...AU1 CMV Friedreich ataxia Michael Ristow 14977 pCINeo-hFX(I154F)-HA FXN HA, AU1 CMV Friedreich ataxia ... hFXN Friedreich ataxia Michael Ristow 14979 pGL3-basic-hFX-promotor(1,3kb) FXN hFXN Friedreich ataxia...distal) FXN hFXN Friedreich ataxia Michael Ristow 14987 pBlue-hFX-HA FXN HA, AU1 T7 Friedreich ataxia Michael..., AU1 T7 Friedreich ataxia Michael Ristow 14989 pBlue-hFX(T133A)-HA FXN HA, AU1 T7 Friedreich ataxia Michael... AU1 T7 Friedreich ataxia Michael Ristow 14991 pET28(a)-hFX-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael...

  17. Chemogenetics AAV Preps

    Type
    Collection
    ...field. Receptor hM3D(Gq) DREADD rM3D(Gs) DREADD Inhibitory hM4D(Gi) DREADD KOR DREADD PSAM4 GlyR Promoter ... mCherry fusion none 1, 9 Roth 83896 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 hM4D(Gi) - Inhibition NLS-dTomato...NLS-dTomato none 1, 9, rg* Fishell 83897 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 hM3D(Gq) - Activation NLS-dTomato...mCherry fusion none 5 Roth 177672 pAAV-nEF-Con/Fon DREADD Gi-mCherry rM4D(Gi) - Inhibition mCherry Cre and...Flp-dependent 8 Deisseroth 177673 pAAV-nEF-Con/Foff DREADD Gi-mCherry rM4D(Gi) - Inhibition mCherry Cre-dependent...

  18. Caltech Systemic Capsids

    Type
    Collection
    ...Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent DREADD Roth 135635... iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase iDreV Zeng 140137 pAAV-EF1a-iFlpV ...-Gq-P2A-dTomato E2 regulatory element Activator DREADD Dimidschstein Biosensors 104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE...

  19. Chemogenetics Plasmids

    Type
    Collection
    ...Designer Drugs (DREADDs) and Pharmacologically Selective Actuator Modules (PSAMs). DREADDs are the most ...inventory of chemogenetic constructs, including DREADD constructs. Plasmid...physiologically inert small molecules. There are four types DREADDs in Addgene’s collection: hM3D(Gq), rM3D(Gs), hM4D...

  20. COVID-19 Resources

    Type
    Collection
    ...and Bst polymerase constructs now available from Andrea Pauli's lab. Neutralizing nanobodies bind SARS-...reverse transcriptase expression plasmids from the Drew Endy and Philippa Marrack Labs. Point-of-care testing...new window) Reverse Transcriptase plasmids from Andrew Ellington's lab. High-surety isothermal amplification...in a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid tools that may be...
Showing: 1 - 20 of 336 results