We narrowed to 101 results for: fluorophore
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TypeCollection...HaloTag protein, labeled with the NanoBRET 618 fluorophore, as the energy acceptor. Live-cell reagents allow...
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Visualizing Protein Turnover In Situ
TypeBlog Post...Which is best for me? Learn how small molecule fluorophores can be used for cell labeling Check out other... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...Fluorescent Protein eBook Learn about Small Molecule Fluorophores Resources on Addgene.org Visit Our Fluorescent... -
Antibodies 101: Affinity Reagents
TypeBlog Post...and cells. They can be used either with or as fluorophores in a wide variety of colors. To antibody or... the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...expression of transgenes of interest. Furthermore, fluorophore variants (for example green and red fluorescent... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post.... G-baToN can also be applied to other fluorophore/antibody pairs. Because the anti-GFP nanobody... -
Plasmids 101: FLEx Vectors
TypeBlog Post... of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of the FLEx system... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...change to large insertions like the addition of a fluorophore or tag. To utilize HDR, a donor template containing... -
Zebrafish Plasmid Collection
TypeCollection...consisting of plasmids with four promoters, six fluorophores, and empty vectors designed to streamline the... -
CRISPR Plasmids - Mammalian Expression
TypeCollection... include both unique and repetitive regions. Fluorophores may also be fused to gRNA sequences to produce... -
Fluorescent Protein Guide: FRET
TypeCollection...non-radiatively transferred from an excited donor fluorophore to an acceptor. Since the transfer of energy... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post... fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash and pcDNA3.1...photon energy is transferred to the acceptor fluorophore, a circularly permuted Venus. In the case of ...of pHlash, the signal from the fluorophore is pH-dependent. Signal is simultaneously acquired from the ...fluorescence in the presence of DHFBI, a small molecule fluorophore. When fused to an RNA of interest, Broccoli-DHFBI... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post...significantly brighter and available with red-shifted fluorophores (find FRET capable GEVIs here!). The only issue... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post...responses of cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011; Matsuoka... -
Neuronal labeling with Spaghetti Monster
TypeBlog Post...contrast, smFP tags can be recognized by distinct, fluorophore-labeled antibodies with very specific reactivities... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the inter- and intramolecular... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...flexibility in color labeling with exogenous fluorophores, which can even be bright enough for single-... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...will be cleaved resulting in activation of the fluorophore. The fluorescent signal can be used as an indicator... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...but rather because of the correct folding and fluorophore formation by most, if not all, of the FP fusions...