We narrowed to 99 results for: fluorophore

1. Visualizing Protein Turnover In Situ

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   ...Which is best for me? Learn how small molecule fluorophores can be used for cell labeling Check out other...

2. Antibodies 101: Affinity Reagents

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   ...and cells. They can be used either with or as fluorophores in a wide variety of colors.  To antibody or... the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction...

3. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   ...expression of transgenes of interest. Furthermore, fluorophore variants (for example green and red fluorescent...

4. Hot Plasmids and Viral Preps - March 2021

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   ....   G-baToN can also be applied to other fluorophore/antibody pairs. Because the anti-GFP nanobody...

5. Plasmids 101: FLEx Vectors

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   ... of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of the FLEx system...

6. Zebrafish Plasmid Collection

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   ...consisting of plasmids with four promoters, six fluorophores, and empty vectors designed to streamline the...

7. CRISPR Plasmids - Mammalian Expression

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   ... include both unique and repetitive regions. Fluorophores may also be fused to gRNA sequences to produce...

8. Fluorescent Protein Guide: FRET

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   ...non-radiatively transferred from an excited donor fluorophore to an acceptor. Since the transfer of energy...

9. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   ...change to large insertions like the addition of a fluorophore or tag. To utilize HDR, a donor template containing...

10. 28 Hot Plasmid Technologies from 2015

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    ... fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash and pcDNA3.1...photon energy is transferred to the acceptor fluorophore, a circularly permuted Venus. In the case of ...of pHlash, the signal from the fluorophore is pH-dependent. Signal is simultaneously acquired from the ...fluorescence in the presence of DHFBI, a small molecule fluorophore. When fused to an RNA of interest, Broccoli-DHFBI...

11. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...

12. Viral Vectors 101: Voltage Indicators

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    ...significantly brighter and available with red-shifted fluorophores (find FRET capable GEVIs here!). The only issue...

13. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the inter- and intramolecular...

14. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    ...will be cleaved resulting in activation of the fluorophore. The fluorescent signal can be used as an indicator...

15. Neuronal labeling with Spaghetti Monster

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    ...contrast, smFP tags can be recognized by distinct, fluorophore-labeled antibodies with very specific reactivities...

16. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    ...flexibility in color labeling with exogenous fluorophores, which can even be bright enough for single-...

17. Magnetic Control of Proteins: More than a Dream

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    ...responses of cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011; Matsuoka...

18. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    ...but rather because of the correct folding and fluorophore formation by most, if not all, of the FP fusions...

19. Plasmids 101: Protein tags

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    ...tag for purification? Or you want to combine a fluorophore with a tag that localizes your protein to the...

20. Hot Plasmids: Fall 2024

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    ...the intestine. Figure 3: Expression of fluorophore-tagged GOLG-4 through eight stages of embryonic...