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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 318 results for: idua

Showing: 61 - 80 of 318 results

  1. Antibody Guide

    Type
    Guide
    ...variety of antibodies to be produced in a single individual, giving a high probability that an antibody will...as immune responses have high inter- and intra-individual variability. Other Affinity Reagents There are...sonication process may need to be optimized for individual assays. Clear cell debris by sedimentation. Proceed...use a nuclear stain, such as DAPI, to identify individual cells. These stains also emit fluorescent emissions...excitation, and assay readout vary widely depending on individual assay conditions. Controls for visualization ...experiments often use a nuclei marker to identify individual cells and additional markers to distinguish cell...

  2. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ... hopes that these screens will both identify individuals who are infected with HR HPV and therefore at...HPV subtypes with risk and disease severity. Individuals who are shown to be at high risk by these screens...

  3. Open Enzyme Collection

    Type
    Collection
    ...assembly. Plasmids Explore the collection and order individual plasmids via the links below: DNA Ligases DNA...

  6. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...disease progression or onset. These hits can reveal individual genes or sometimes even genetic pathways that...generate patient mutations and then test both individual and combinatorial drugs on for effectiveness ...

  8. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ... a survey instrument designed to measure how individuals respond to issues of unsettled science. Understanding...building, each day brushing past and chatting with individuals whose research ranged from wildlife conservation...

  9. RUBY-Red Siliques

    Type
    Blog Post
    ...your selection method kills the transgene free individual. For example, with CRISPR-Cas9 gene editing,...experiments can only be collected from heterozygous individuals. Only 1/4 of the descendants of the heterozygous...

  11. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...web-based, career-planning platform called the Individual Development Plan (IDP) and uses it to match qualified...interests: Use career planning tools such as the Individual Development Plan to generate a list of potential...

  12. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...titers Making contact with a cell Although an individual AAV particle’s ability to infect a cell is dependent...high titers, as contact between the cell and any individual AAV particle is rare. But simply getting into...

  13. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...expression of the array. After expression, the individual gRNAs are released by endogenous tRNA processing...which is followed by tRNA processing to release individual gRNAs for gene editing. They named this new array...

  14. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...ends. This homology can be contained within the individual fragments themselves, but doesn’t necessarily... challenges can be circumvented by generating individual fragments containing mutations and aligned via...

  16. The MTA at Addgene

    Type
    Blog Post
    ...you ask? Well, organizations used to negotiate individual MTAs every time they wanted to share materials...Addgene — basically, instead of having to sign individual MTAs for each request, the depositing organization...

  17. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... left the arduous task of virus production to individual labs, but now we’re very excited to provide ready-to-use...uses nearly 80,000 gRNAs to target around 20,000 individual genes. We provide enough lentivirus for you to...

  18. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...DNA is harvested, much like one would prep an individual plasmid. However, lots of library DNA is needed...obtain about 100 to 1,000 bacterial colonies per individual plasmid in the library. Bacterial growth must...

  19. Components of CRISPR/Cas9

    Type
    Blog Post
    ... Cas9 endonuclease or a variant thereof. This individual protein has all the components necessary to: ...foreign DNA target sequences specific to each individual crRNA. Bottom line, the “direct repeat region...

  20. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...in real time PCR, each reaction partition is individually assessed for a positive or negative (digital...resolution of digital PCR allows classification of individual target molecules based on their mutation status...
Showing: 61 - 80 of 318 results