We narrowed to 242 results for: kit
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TypeCollection...Gate TALEN kit. In this example, the final array is composed of 18 TAL effector repeats. The kit can be used...Bogdanove TALEN kit came online in the summer of 2011 and has already become the best-selling kit in Addgene...Hospital also recently released a TALEN kit through Addgene. This kit, first described in Nature Biotechnology...to TALEN Technologies You may also like... TALEN Kits CRISPR Plasmids Zinc Finger Consortium Originally...” Using these plasmids, which are available as a kit from Addgene , custom arrays consisting of 12-31 ...considerable experience in the DNA targeting field. A third kit, a "TALe Toolbox", from Dr. Feng Zhang’s group at...Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in a 2012 issue...
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Plasmid Collections
TypeCollection...Biology Tet Inducible Expression Worm Expression Kits Kits are groups of plasmids that have been packaged...intended for use as a set. Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering...Browse Addgene’s curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent... . We've gathered our curated plasmid resources, kits, and depositor collections on one page to help you... part of a pathway or ORF. Find a Pathway or ORF Kit Disease Research Find deposited plasmids for specific... -
Synthetic Biology - Overview
TypeCollection...PUF Assembly Kit Optogenetics Pertz Lab cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae...Lab Logic Gates , BIOFAB Promoter/BCD Kit , and pOSIP Plasmid Kit Gray Lab Maize and Rice Transcription...Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular... -
Antibodies 101: Conjugation
TypeBlog Post...looking to conjugate antibodies yourself, there are kits available to make the process quick and easy. Before...antibody’s buffer is compatible with your conjugation kit, since many common antibody buffers and buffer additives... -
Click Biology: How to Click and Play to Explore Biology
TypeBlog Post...CRISPaint Gene Tagging Kit SpyTag/SpyCatcher CRISPR-assisted insertion tagging Kit; integration of large... -
CRISPRainbow and Genome Visualization
TypeBlog Post...little more colorful? CRISPRainbow is available as a kit from Addgene. References 1. Chen, Baohui, et al...Fluorescent Protein Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog Post...Sandwich ELISA Most commercially available ELISA kits are for a sandwich ELISA. This technique works by...interested in detecting in your sample. When an ELISA kit is ordered, the wells are typically already coated... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth’s lab also assembled...ligand-bound GPCRs of interest. The TRUPATH plasmid kit consists of 20 plasmids encoding 14 alpha, 2 beta... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post...there are several commercially available p24 ELISA kits that can quantify p24 levels in a matter of hours...day, but tends to be less expensive than an ELISA kit. In this approach, viral RNA is first converted to... -
Twenty Years of Sharing Science!
TypeBlog Post...repository for DNA-based materials, including our popular kits and libraries. This DNA-based materials repository... -
Distribution to Industry
TypeCollection...Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning ...Industry Featured Collections Search Plasmids Plasmids Kits Pooled Libraries Antibodies Ordering and MTA Information...Cloning Eric Cantor Kit for using comprehensive end-joining ligation fidelity and bias data to predict high ...eukaryotic multigene constructs. ONE-GO Biosensors Kit Fluorescent Proteins Mikel Garcia-Marcos 10 plasmids...of cell types. Protein Purification Optimization Kit Using SUMO Protease Elution Protein Characterization... junction sets for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post... you prepaid shipping materials (i.e., a deposit kit) with instructions on how to send liquid DNA or bacterial...pages for your review. We’ll also mail you a deposit kit with instructions on how to prepare and ship the ... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post... colonies, sub-culture, and prep with a DNA prep kit or your lab's favorite in-house protocol. DNA yields...lead to degradation of your prep. Many plasmid prep kits recommend performing an extra wash step to remove... -
Twelve Amazing Guest Blog Contributors!
TypeBlog Post...and Plasmids 101: Modular Cloning Applications and Kits We are very grateful for each and every contributor... for Protein Innovation. An Integrin Antibody Toolkit from IPI Simon Moe, from Promega, who wrote a ... -
Kit Free RNA Extraction
TypeProtocol... Protocols RNA Extraction Without a Kit RNA Extraction Without a Kit You may also like...extraction without a kit. Protocols... like... Kit-Free DNA Purification Agarose Gel Purification Molecular Biology Reference Introduction This...how to extract RNA from tissues or cells without a kit. The general steps of this protocol include homogenization... read this blog post on RNA extraction without a kit . Last Update: April 10, 2020 Equipment Refrigerated... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog Post...mutant cell lines. Further, for each plasmid in the kit, the transcript most commonly found across all TCGA...Tackling Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used in ... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...Addgene.org Check out the SapTrap Kit Page Find Other Fluorescent Protein Kits Browse the Fluorescent Protein... recently developed a modular plasmid assembly toolkit called SapTrap to improve CRISPR genomic tagging...offers for tagging a single genetic locus, the toolkit also offers repair templates for tagging proteins... elegans, several groups have designed modular toolkits to assist with tagging genomic loci in other model...obtained as a universal donor plasmid. The CRISPaint toolkit includes several donor plasmids for protein tagging... S2 cell line stably expressing Cas9. This PCR toolkit offers C- and N-terminal tagging vectors (eg GFP... Matthew L. and Eric M. Jorgensen. “SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post...used in this study. The zCas9i cloning kit is a plant cloning kit that contains a series of vectors and... -
Bringing Renewable Energy to Addgene
TypeBlog Post...With a collection of over 70,000 plasmids, kits, and viral service items we employ dozens of pieces of...sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple streams for composting... -
Protocol - How to Purify DNA from an Agarose Gel
TypeProtocol... purification kit, such as the (Link opens in a new window) QIAquick Gel Extraction Kit . Always follow...important during the DNA purification step, as many kits cannot handle more than a certain total volume of...