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We narrowed to 194 results for: lenti

Showing: 61 - 80 of 194 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with just the...to create a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed...collection of plasmids comprising the multiple lentiviral expression (MuLE) system from the laboratory ...the process of creating complex polycistronic lentiviruses. The MuLE toolbox contains a series of building-block... combinations of genetic elements in a single lentiviral vector provides a powerful tool to modify mammalian...routes in various conditions. The plasmids and lentiviral vectors encoding these proteins can also be used...

  2. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...Blog Learn More about Lentivirus Figure out How to Get the Most from Your Lentiviral Transduction Additional... individually overexpressed and available for lentiviral packaging and delivery to target cells as a pooled... Fig. 1: Barcoded TF library was packaged in lentiviral particles and used to transduce human embryonic...

  3. Hot Plasmids - January 2023

    Type
    Blog Post
    ... A novel RNA targeting system Heat-inducible lentiviral Car constructs Anti-GFP [N86/8R] ready-to-use...of print. PMID: 36690762.   Heat-inducible lentiviral CAR constructs by: Brian O'Neill  A clever system...crux of the system is a set of heat-inducible lentiviral CAR constructs, the most basic of which is depicted..., inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the...

  4. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ... CRISPR pooled libraries have been created in lentiviral systems for mammalian screens, which enables ...However, some cell types are poorly transduced by lentivirus, and thus screening with available CRISPR libraries...Primary murine T cells cannot be transduced by lentivirus and have been difficult to manipulate genetically...for screening of cells that are refractory to lentiviral transduction, now enabling forward genetic screens...

  5. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...different types of tubes: the ones we use to store lentivirus and the ones we use to store AAV. Our results...effect on the pH of the samples stored in our lentivirus tubes (Fig. 1). However, once thawed, there was... 1st Time CRISPR User) Tips for Titering Your Lentivirus Preps Additional Resources on Addgene.org Check... lentivirus...

  6. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...conducted earlier by the Zhang lab for GeCKO, a lentiviral CRISPR library enabling genome-scale, knockout...that you review the steps for how to use pooled lentiviral CRISPR libraries and keep the following in mind... sequencing technology. These libraries are lentiviral in nature, so please ensure that you are equipped...equipped and authorized to make and use lentivirus. Also, note that due to the size of the backbone, transformations...

  7. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...published a protocol and reagents for pseudotyping lentivirus with the SARS-CoV-2 spike protein. They deposited...19 pandemic, the Krogan lab at UCSF generated lentiviral vectors expressing SARS-CoV-2 open reading frames...all but one are cloned into pLVX vectors (for lentiviral packaging) and contain an IRES-Puro marker for...(2020) Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization...

  8. Cre-lox system

    Type
    Collection
    ...12238 pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-...coexpression CMV Lentiviral Sadelain 30205 Cre-IRES-PuroR Cre and PuroR coexpression EF-1 alpha Lentiviral Kotton...-cre mCherry and Cre Lentiviral Luikart 66691 FUG-T2A-cre GFP and Cre Lentiviral Luikart 66692 pRubiC-...-iCre copGFP and iCre CB Lentiviral Oka 72257 pCDH-CB-iCre iCre CB Lentiviral Oka 73350 pAdx-CMV-iCre-... falxiparum Spielmann 86641 pLenti-hSynapsin-Cre-WPRE Cre hSyn Lentiviral Wang 86794 pCL20c-MSCV-IRES-...Eisenhoffer 131786 pLenti-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector Cbh...Cre, Puro resistance and miRNA expression PGK Lentiviral Jacks 17736 pOG231 NLS-Cre CMV Mammalian Wahl...

  9. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...parentheses.  In this example I wanted to find a lentiviral plasmid to express ubiquitin under control of...300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed...this filter will allow to refine by serotype or lentiviral prep Requests: Only see plasmids with more than... cell type Serotype: Choose the AAV capsid or lentiviral prep needed Tags: Identify a specific marker ...

  10. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...their options for tools such as empty backbones, lentiviral packaging systems, and luciferase expression.... Top Five by Views Guides CRISPR Guide Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus...a Liquid Bacteria Culture Collections Lentiviral Plasmids Tetracyline Inducible Plasmids Empty...help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the protocols ...

  11. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...within the lentiviral 3’ long terminal repeats (LTR). The 3’ LTR is duplicated during lentiviral integration...license. In this iteration, the size of the lentiviral insert was minimized to reduce recombination ...III transcript, the sgRNAs were encoded on the lentiviral minus strand in the 3’ LTR. These optimizations...

  12. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...purpose. For mammalian lentiviral expression, we could use a vector like pLenti CMV Puro DEST (w118-1)...Gateway cloning to generate our desired constructs: lentiviral expression of the human KRAS gene in mammalian...available at Addgene, including the Frew Lab Multiple Lentiviral Expression Systems (MuLE) Kit, the MultiSite ...

  13. Viral Production

    Type
    Collection
    ...AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced in 293T ...performed on lentiviral preparations that have been stored at -80 °C and thawed. Lentivirus titration is...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...viral production methods and quality control for lentivirus and AAV. Viral...AAV Production Titer Quality Control Resources Lentivirus Production Titer Quality Control Resources Overview... Cell culture medium (OptiPro SFM) containing lentivirus is first cleared by low-speed centrifugation ...cells are transduced with serial dilutions of a lentiviral vector, incubated for 72 h, and genomic DNA is...

  14. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ... NEB Stable   For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated...JM109-derived. For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated...Derived from HB101. For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated...cells for cloning and storage of unstable DNA and lentiviral/retroviral vectors. Used by Addgene for pooled...

  15. Tetracycline Inducible Expression

    Type
    Collection
    ...Description Element On or off PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral reverse tetracycline-controlled...Resources: Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated Plasmid Collections Background...On or Off PI 21916 Tet-pLKO-neo 3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin... selection TetR On Wiederschain 41393 pCW57.1 Lentiviral vector for inducible expression for Gateway cloning...insert options rtTA On Root 11651 pLVUT-tTR-KRAB Lentiviral vector for inducible expression of transgene ...tTA or rtTA None On Vogelstein 25735 pSLIK-Neo Lentiviral vector for inducible expression of a miR-shRNA...third-generation rtTA On Fraser 44012 pInducer20 Lentiviral vector for shRNA expression; see article for ...

  16. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...., BMC Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems...upon the CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing...Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...CoChR have been deposited to Addgene, including lentiviral and AAV expression vectors for certain variants...purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor plasmids from ...

  17. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...available in two vector formats: all-in-one (lentiCRISPRv2, expressing both Cas9 and an individual gRNA...gRNA from this single vector) and guide-only (lentiGuide expressing only an gRNA, for use with cells that...at Addgene, you can also order the individual lentiGuide gRNA plasmids targeting the human kinome. These...

  18. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...(Rosa26-Cas9) hematopoietic stem cells with a lentiviral vector containing a gRNA. These transduced stem...systems which utilizes custom mouse strains and lentiviral expression vectors (Table 1). The four systems...pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the first generation CHIME ...

  19. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...perform the transduction with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to... some viruses integrate into the host genome (lentivirus and retrovirus) while others are non-integrating...Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral Services...
Showing: 61 - 80 of 194 results