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We narrowed to 26 results for: lenti

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Showing: 1 - 20 of 26 results

  1. Lentivirus Plasmids

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    Collection
    ... safety. Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids ...at Addgene. Read our Lentiviral Vector Guide for more information about lentiviral components, generations... Additional Resources Lentiviral Vector Guide Addgene’s Viral Service Lentiviral Pooled Libraries All ...Addgene lentiviral packaging, envelope, and transfer plasmids Viral... Viral Plasmids Lentiviral Plasmids Lentiviral...Packaging Plasmids Envelope Plasmids Resources Lentiviruses, a subset of retroviruses, are some of the most...and useful types of viruses used in research. Lentivirus can transduce both dividing and non-dividing ...

  2. Lentiviral Prep Service

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    Collection
    ...GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled ...sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114...gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes...sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637...Resistance Purpose PI 17446 pLenti CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...

  3. Kazuhiro Oka Lentiviral Vectors

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    ...deposited a series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors ...vectors. Download the Lentivirus Transfer Vector Production Protocol Lentiviral Vector ID Purpose pCDH-EF1...For more information on lentiviral and adenoviral vectors read our Lentiviral Vector Guide and Adenoviral... in our Lentiviral Plasmids and Adenoviral Plasmids collections Addgene Blog: Your Lentiviral Plasmid ...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Depositor... Viral Vectors Gene Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral Transfer Vectors ...Vectors Within this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not...

  4. SARS-CoV-2 Pseudotyped Virus

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    ...these cells. Warner Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly...Christopher Vakoc 138152 pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro Lentiviral vector expressing destabilized...examples of each in Addgene's collection: HIV-based lentiviral particles Murine leukemia-based retroviral particles...Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus...Description PI 44965 NL4-3 mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly... Sanjana 164432 pHAGE-CMV-Luc2-IRES-ZsGreen-W Lentiviral dual reporter vector expressing firefly luciferase...pBOBI-FLuc Express firefly luciferase. Used in lentivirus-based SARS-CoV-2 related pseudovirus assay. David...

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ... Mammalian lenti sgRNA(MS2)_zeo backbone - 3rd gen lenti sgRNA cloning backbone with MS2 loops at tetraloop... genome pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene expression pLenti CMV/TO ...pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers...Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by...Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector for mammalian genome editing... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...

  6. Luciferase Plasmid Collection

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    ...luciferase Scott Lowe 105621 Lenti-luciferase-P2A-Neo Firefly EF1α Lentiviral expression of firefly luciferase...fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2-Nanoluc-ccdB NanoLuc® Creation ...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...luciferase Eric Campeau 21474 pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly luciferase...Firefly Ubc Lentiviral expression of firefly luciferase Linzhao Cheng 74444 pLenti.PGK.blast-Renilla_Luciferase...Marcel Bruchez 108542 pLenti-EF1a-Luciferase-IRES-Blast-WPRE Firefly EF1α Lentiviral expression of firefly... tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc (neo) Akaluc hPGK Lentiviral expression of Venus-Aka-...

  7. Neurodegeneration Plasmid Collection

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    ...Bonifacino 173579 Lenti-sgAtm#1/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ...Venus CMV Lewy body dementia Tiago Outeiro 89643 Lenti-sgAtm/Cre ATM U6 Ataxia telangiectasia Monte Winslow...FusionRed CUP1 ALS Michihiko Kataoka 188460 pKDR-Lenti-CBh.6-SOD1 SOD1 CBh ALS Erik Dent 188572 Tet-off...LRRK2 His T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia...Cookson 13318 pLenti6-DEST PINK1-V5 G309D PINK1 V5 CMV Parkinson's Mark Cookson 13319 pLenti6-DEST PINK1-... Cookson 29412 pLenti6-DJ1-V5-E18N PARK7 V5 CMV Parkinson's Mark Cookson 29413 pLenti6-DJ1-V5-C106D PARK7...Cookson 29414 pLenti6-DJ1-V5-L166P PARK7 His, V5 CMV Parkinson's Mark Cookson 29415 pLenti6-DJ1-V5-E18D ...

  8. Plasmids for Stem Cell Research

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    ... 11. 3(3):346-53. Jaenisch Lentivirus Human Single polycistronic lentiviral vector for the expression ...May . 27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral vector for the expression ...1221-9. Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible lentiviral vector for the ...2):151-9. Jaenisch Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression.... 106(1):157-62. Jaenisch Lentivirus Mouse Doxycycline-inducible lentiviral expression of mouse Oct4, ...7239):766-70. Nagy Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression...Delivery Method Species Description Article PI Lentivirus Human Expression of human Oct4, Sox2, Klf4, Myc...

  9. Tetracycline Inducible Expression

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    ...Description Transactivator PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral Tet-On vector with blasticidin...rtTA-Advanced Mikhail Alexeyev 17492 pLenti CMV TetR Blast (716-1) Lentiviral Tet-On vector expressing TetR ...87360 TLCV2 Lentiviral vector for tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam ...Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression of shRNA...2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design ...-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA...rtTA3 Ptet, miniCMV Howard Gu 44012 pInducer20 Lentiviral Tet-On vector for inducible expression. See article...

  10. Viral Production

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    ...AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced in 293T ...performed on lentiviral preparations that have been stored at -80 °C and thawed. Lentivirus titration is...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...viral production methods and quality control for lentivirus and AAV. Viral...AAV Production Titer Quality Control Resources Lentivirus Production Titer Quality Control Resources Overview... Cell culture medium (OptiPro SFM) containing lentivirus is first cleared by low-speed centrifugation ...cells are transduced with serial dilutions of a lentiviral vector, incubated for 72 h, and genomic DNA is...

  11. Zhang Lab CRISPR Page

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    ...system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (...#61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding dCAS9-VP64 with... top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries (both human and mouse) for performing...with 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper complex...complex with a 2A Hygro resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop...

  12. CRISPR Guide

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    ... libraries. What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available...

  13. Mammalian RNAi Tools

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    ...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more....Addgene viral protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized...shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...for cloning in shRNAs. Most plasmids are in a lentiviral backbone, with some retroviral and AAV backbone...

  14. Plan Your Experiment

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    ...production, see our viral vector guides . Lentiviral vectors Lentiviral vectors are a popular option for producing...types like primary cells. For CRISPR delivery, lentiviral and adeno-associated virus (AAV) vectors are ...CRISPR components) integrates into the genome. Lentiviral vectors are a good option for difficult-to-transfect...we don’t discuss CRISPR screens in this guide, lentiviral vectors are a common choice for conducting genome-wide...CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages...advantages for viral vector delivery. Both lentiviral and AAV vectors are safe to use in the lab, but ...into your viral vector) is smaller than that of lentivirus. Thus, AAVs are generally only compatible with...

  15. CRISPR References and Information

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    ...amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro packaging plasmids...CRISPOR batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new...D10A (pX335) PDF, 255 KB Zhang sgRNA cloning lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive...

  16. Fluorescent Protein Guide: Empty Backbones

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    ...pmiRFP670-N1 - Mammalian Expression pLenti6.2_miRFP670 - Mammalian Lentiviral Expression pBAD/His-miRFP670 -...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression... Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+8CmCherry - Zebrafish/Xenopus...smURFP 642 670 32 3.2 39 min Prone to dimerization pLenti-smURFP - Mammalian Expression Return to top Near-Infrared...

  17. Viral Vector Guides and Plasmids

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    ...Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile ...Infected Host Genome Integration Immune Response Lentiviral Stable RNA < 8 kb 80–130 nm Dividing/Non-dividing...

  18. CRISPR Plasmids - Empty gRNA Vectors

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    ...Multiplex CRISPR/Cas9 Assembly Systems Multiple Lentiviral Expression System Kit Fragmid Kit MoClo CRISPR...visiting our CRISPR reference page . CRISPR Guide Lentiviral Preps CRISPR Protocols gRNA Design Tools CRISPR...

  20. Neurodegeneration Research Collection

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    ...more. Convert iPSCs to neurons with inducible lentiviral expression constructs: pLVX-UbC-rtTA-Ngn2:2A:...transcription factors in a single polycistronic lentiviral vector . (Addis et al. PLoS One. 2011) See More...
Showing: 1 - 20 of 26 results