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We narrowed to 80 results for: lentiviral infection

Showing: 61 - 80 of 80 results

  1. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...screen. We start by infecting millions of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated...retrovirus in the same family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the ...cells. Remove this pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing...

  2. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...transduced with Cas9 expressing lentivirus at the indicated multiplicity of infection (MOI). Anti-vinculin Western...performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic overview of CRISPR...stably expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1)....Lines with Lentivirus Isolating a Monoclonal Cell Population by Limiting Dilution Lentivirus Production...

  3. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...applications Viruses work by infecting a host cell (the target of viral infection) and delivering a genetic...high infection frequency and the viral titer (the number of viral particles capable of infecting a host...is more than one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided ...

  4. Important Considerations When Using AAVs

    Type
    Blog Post
    ... possible elicited immune response from viral infection. Animal safety is a top priority in our lab so...which determines which tissues and cells an AAV can infect) can influence an AAV’s value greatly. Applying...largely due the ability of different serotypes to infect different cell types. In the CNS, it has been shown...), which is a possible mechanism for the AAV to infect other cell types not within the vicinity of the...virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal of virology 79.15...facilities (Biosafety Level-1). Other possible tools, lentiviruses for example, are not approved for use in vivo...viruses, so they are resistant to alcohol-based disinfectants. A 10% bleach solution should be used to clean...

  5. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...in functional virions. These viral particles can infect other cells, but since the majority of the viral...then be placed on whatever cells you would like to infect with the virus. Then the viral particles do the...perform the transduction with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to... some viruses integrate into the host genome (lentivirus and retrovirus) while others are non-integrating...Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral Services...

  6. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Bergelson S, Feschenko M. Determination of Lentiviral Infectious Titer by a Novel Droplet Digital PCR Method...be close to zero (A01). In this protocol, the lentiviral particles are serially diluted and used to transduce... of Lentivirus Vectors You may also like... Lentivirus Colony Formation Titration Assay Lentivirus Fluorescence...genome to calculate the infectious titer: $$T = {V*C*D\over v}$$ Where: T = Infectious titer, TU/mL V = Viruses...average infectious titer obtained from the appropriate dilutions to calculate the final infectious titer... PCR (ddPCR) for lentivirus vector titration. Protocols... Protocols ddPCR Titration of Lentivirus Vectors ddPCR Titration...

  8. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...these methods are naturally occurring viruses that infect, persist, and migrate within neurons, and that ... moves retrogradely from a peripheral site of infection to the central nervous system where it replicates... added benefit of only labelling the subset of infected cells that also express Cre. Another advantage...vectors into a brain region, the AAV particles will infect neurons at different ratios, making these neurons...intracellular transport without actually causing infection or leading to transgene expression within the ...). AAV does not have a membrane envelope like lentivirus, but instead is contained by a capsid which is...

  9. Lentiviral Vector Guide

    Type
    Guide
    ... Guides Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...gamma-retroviruses can only infect mitotically active cell types. This means that lentiviruses can infect a greater variety... Things Lentivirus Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production...safety. For a summary of all lentiviral plasmid components, see the Lentiviral Plasmid Elements table . In... a wide range of infectivity for different cell types (known as tropism). Lentiviral vectors have gone...different generations. Lentiviral Generations First-generation First-generation lentiviral plasmids are largely...

  10. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...gamma-retroviruses can only infect mitotically active cell types. This means that lentiviruses can infect a greater variety...plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide...difference between lentiviruses and gamma-retroviruses is that lentiviruses are capable of infecting non-dividing... to the Retroviridae family. Gamma-retrovirus infection is associated with many serious illnesses. Common...gamma-retroviruses may not be efficiently packaged by lentiviral packaging systems, and vice versa. Gamma-retroviral...packaging gene env ; usually VSV-G due to wide infectivity VSV-G (vesicular stomatitis virus G protein) ...common envelope gene, due to a wide range of infectivity for different cell types (known as tropism). ...

  11. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...categories (e.g., for a lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression...be prone to recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the...that I have disclosed all known hazardous and infectious properties of the submitted materials. As the...

  12. Guide to Using Pooled Libraries

    Type
    Guide
    ... are infected at a very low multiplicity of infection (MOI) to increase the odds that an infected cell...cells of interest. With others, notably pooled lentiviral plasmid libraries, the plasmids must first be...adequately represented in the population, you’ll need to infect many more cells than the number of plasmids present...Examples of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...delivering as virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells... do not survive the selection mechanism. You’ll infect two sets of cells and subject one set to selection...libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...

  13. Immunology Research Plasmids and Resources

    Type
    Collection
    ...kill cells that have been infected by a foreign pathogen. They detect infected cells via receptors such...construct complex lentiviruses for modification of mammalian cells with a single viral infection from the lab...pathogens each day. Their ability to protect against infection depends on two systems that work in concert: the... the innate and adaptive immune systems. Upon infection, the immune system triggers several processes ...specific antigen, it finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced...destruction of pathogens. Over the course of an infection, adaptive immune cells will expand and give rise...

  14. CRISPR Guide

    Type
    Collection
    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...

  15. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...Berkeley engineered an AAV variant that efficiently infects neurons, directs high-level transgene expression...Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral Service ...Ready-to-use Virus for Your Research Visit Our Lentivirus Guide Page ...

  16. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...viral tropism—the type of cells the viral particles infect. This can direct your biosensor to specific tissues...ready to deliver your sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we...

  17. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...different concentrations of virus, or multiplicity of infections (MOIs), to determine the optimal dose of AAV ...always make more AAV than I think I need. Unlike lentivirus, AAV requires a higher number of viral particles...Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols Find AAV ...

  18. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...or determination of the median tissue culture infective dose (TCID50) (Zen et al., 2004).  To choose the...production process.  Helper-free production Unlike lentivirus, AAV does not possess the ability to replicate...are measured. A TCID50 measurement reports an infectious titer, meaning the concentration of viral particles...2010). It is recommended that you measure your infectious titer, since it will have more impact on your...Adeno-Associated Viral Vectors by Oxidant-Based Disinfectants. Human Gene Therapy, 32(13–14), Article 13–14...Bleu, T., Sommer, J. M., & Wright, J. F. (2004). Infectious titer assay for adeno-associated virus vectors... with sensitivity sufficient to detect single infectious events. Human Gene Therapy, 15(7), Article 7....

  19. Molecular Biology Reference

    Type
    Guide
    ...particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that can infect your target cells...viral service offers select ready-made AAV and lentiviral particles. Visit our viral service page to learn...

  20. CRISPR Guide

    Type
    Guide
    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...
Showing: 61 - 80 of 80 results