We narrowed to 92 results for: mpi
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TypeBlog Post...can be a very fulfilling experience; even the grumpiest of researchers can enjoy it at times! What makes...
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Antibodies 101: Reading a Flow Plot
TypeBlog Post...Gonzalez-Martinez, G., Alfaro, R., Galián Megías, J. A., Campillo, J., Moya-Quiles, M., Minguela Puras, A., Pons... -
Career Insights: Technical Support Specialist
TypeBlog Post...can be highly rewarding but can also be a good jumping off point for other roles in industry. Many thanks... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...saturation density, decreased rates of growth, or clumping. To ensure the best possible results for any tissue... -
Plasmids 101: Control Plasmids
TypeBlog Post...individual or co- transfection, should be determined empirically. Negative controls: untreated cells, empty vector... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post...Expression of unstable proteins HMS174 (DE3)** Rifampicin RecA-deficient; allows for cloning and expression... -
Molecular Biology Reference
TypeGuide...to make 100 mL of LB/ampicillin growth media, add 100 μL of a 100 mg/mL ampicillin stock (1000X stock) ...., a gene whose product confers resistance to ampicillin) is included in the plasmid. These bacteria are... are then grown in the presence of ampicillin. Under these conditions, there is a selective pressure to...Concentration Recommended Working Concentration Ampicillin 100 mg/mL 100 µg/mL Carbenicillin* 100 mg/mL ... *Note: Carbenicillin can be used in place of ampicillin. Preparing Antibiotics Create a stock solution... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...research can be patented? I.e. is automated time stamping (detailed records on who did what and when) important... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...interest. Thankfully, there are a number of websites compiling antibody data that can help you compare antibodies... -
March for Science
TypeBlog Post... education and literacy that this changes. 2) Lumping all scientists (and consequently their results)... -
Evolution of Lab Techniques
TypeBlog Post...sequence, based on which ddNTP was added and when. Compiling the identity and location of a given nucleotide... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...associations across different universities are trying to compile more comprehensive demographic information about... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post... budget can help you compare the monetary and empirical value that helps you determine whether or not ... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...software has two components - a database of pre-compiled register designs and a search function, both of... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post...hpRNAs, they must be individually engineered and empirically evaluated for their gene silencing efficacy, ... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...efficiency than the other and you’ll find out empirically which is the lagging strand. ssDNA substrate ... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...Visaggio, D., Gasperi, T., Parisi, M., Cincotti, G., Rampioni, G., Visca, P., & Kolmakov, K. (2023). Expanding... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post... can vary lot-to-lot, this range needs to be empirically determined every time you order a new lot of ... -
27 Hot Plasmids from 2016
TypeBlog Post...partner regulatory domains. The Chodera Lab recently compiled the Human Kinase Domain Constructs Kit, a library... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...therefore have resistance to both kanamycin and ampicillin (from the pUC19 backbone). *False positives can...