...(PPIs). These interactions are often modeled as binary relationships, i.e. protein A interacts with protein...Split-BioID is that it allows for the validation of a binary PPI as well as the identification of additional...complementation assay that can be used to study binary protein interactions. However, FRET generally only...

...included taking extra coursework and attending seminars in bioethics, public health, science policy, and..., many of us are used to being very focused on narrowly-defined research questions in our field of interest...advantage of their academic environment and attend seminars, symposia, or courses in social, health or policy-related...

...on how to submit your work either through seminars or webinars. Sending on your grant application to your...your work, or even the successful use of your preliminary data to secure further funding. Make it clear...

...Cythina Arokiaraj (Gradinaru lab) Another useful viral tool deposited by the Gradinaru lab, AAV1-X1, was...Hridaya Patel (Addgene co-op) Researchers in the Gradinaru lab have developed an engineered adeno-associated...

...favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway vectors and can be used for promoter swapping...plants. If your cultures have been incubated at stationary phase for too long, the cells in your culture...4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation ...

...modified from a bacterial immune system to a revolutionary tool for genome engineering. CRISPR Topic Page...

...many base editors are designed to work in a very narrow window proximal to the PAM sequence, some base ...

...organismal systems described at this meeting were extraordinarily diverse. Plans to develop tools to study their...electric organs arose in different branches of the evolutionary tree at least 6-7 times and on at least 2 separate... Milkweed plants are known to many as the only Monarch butterfly food. They have chemical pathways generating...

...; multiple codons encode single amino acids. Evolutionary constraints have molded which codons are used...express well in any organism. So what are the evolutionary constraints that lead to these preferences and...6. Pechmann, Sebastian, and Judith Frydman. "Evolutionary conservation of codon optimality reveals hidden...

...Crystal structures of CRBNmidi alone (A) and in a ternary complex with the multiple myeloma drug mezigdomide...CRBNmidi on its own, with drug ligands, and in ternary complexes with other proteins, establishing CRBNmidi...low-expression proteins of interest) and mScarlet-I3 for scenarios requiring rapid maturation and prolonged imaging...

...Nobody EVER complains that a seminar was too easy to understand. Make your seminar accessible. Let the experts...proper consideration. 3. Present an easy to follow seminar See item 2. Attendees will include grad students...

...Roman Jerala Jay Keasling Taek Soon Lee Joshua Leonard Han Lim Wendell Lim Timothy Lu Michel Maharbiz ...

...):47-9. PMID: 17179932 Wickersham IR, Lyon DC, Barnard RJ, Mori T, Finke S, Conzelmann KK, Young JA, Callaway...

...to detect the presence or absence of a protein (Renart et al., 1979, Towbin et al., 1979, Burnett et al...from Addgene that you can make on your own.  To narrow your search, first pay attention to the validated...–939 . https://doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser J, Stark GR (1979) Transfer of proteins...

...Other tools are designed to be activated within a narrower band of wavelengths to allow multiple tools, such... R., Gunaydin, L. A., Hyun, M., Fenno, L. E., Gradinaru, V., Yizhar, O., & Deisseroth, K. (2012). Principles...

...in a new window) - The initiative promotes interdisciplinary exchange, open technologies, and responsible...

... WW domain containing protein 1 SCRIB Scribbled planar cell polarity protein SHC SHC1 SHC2 SHC3 SHC4 SHC...

...communication. Science in the News hosts a podcast, seminars, a blog, conferences, and more for non-scientists...Jessica Welch Have Had to Explain Importance of Narrative in Science Communication Conversation in Science...

...offering an AAV2 CAG-driven tTA expresser from the Gradinaru lab. This is useful for both in vitro and in vivo...serotypes pAAV-CAG-tTA AAV2 Molecular Tools Gradinaru New viral service pAAV_hSyn1_nLightG AAV9 Biosensors...

...senior graduate students, Ben Vincent and Niroshi Senaratne, from the Biological and Biomedical Sciences program...Biology, and he tweets @benjvincent.     Niroshi Senaratne is a graduate of the PhD program in Genetics and...