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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 281 results for: nar;

Showing: 61 - 80 of 281 results

  1. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California Institute of Technology ...Lois, C., Mazmanian, S.K., Deverman, B.E., and Gradinaru, V. (2017). Engineered AAVs for efficient noninvasive..., J.G., Wohnoutka, P., Gerfen, C.R., Koch, C., Bernard, A., Dang, C., Jones, A.R., and Zeng, H. (2014)... PMID: 25240284. Wickersham, I.R., Lyon, D.C., Barnard, R.J.O., Mori, T., Finke, S., Conzelmann, K.-K....

  2. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...computational processing with biological protocols and revolutionary physical approaches. However, with each new ...makerspace networks and training users via workshops, seminars, or hackathons greatly increases accessibility...brings stronger networking across laboratory and disciplinary boundaries and can allow for innovative organizations...

  3. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ... new search tool, are the results of this multidisciplinary effort and cross-team collaboration (thanks...scoping mechanism that allows category-specific, narrow searches. We defined three top level categories... terms in a query in a way that allows users to narrow down search results by adding more terms.  Weighting...

  4. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...considerations will help you narrow down which antibody format to use for your specific scenario.    References and...Fab fragments can be used as a block in similar scenarios. By applying unconjugated Fab fragments that are...

  5. Engaging with science and society at pgEd

    Type
    Blog Post
    ...included taking extra coursework and attending seminars in bioethics, public health, science policy, and..., many of us are used to being very focused on narrowly-defined research questions in our field of interest...advantage of their academic environment and attend seminars, symposia, or courses in social, health or policy-related...

  6. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...(PPIs). These interactions are often modeled as binary relationships, i.e. protein A interacts with protein...Split-BioID is that it allows for the validation of a binary PPI as well as the identification of additional...complementation assay that can be used to study binary protein interactions. However, FRET generally only...

  7. Hot Plasmids February 2024

    Type
    Blog Post
    ...Cythina Arokiaraj (Gradinaru lab) Another useful viral tool deposited by the Gradinaru lab, AAV1-X1, was...Hridaya Patel (Addgene co-op) Researchers in the Gradinaru lab have developed an engineered adeno-associated...

  8. 6 Tips for Grant Writing

    Type
    Blog Post
    ...on how to submit your work either through seminars or webinars. Sending on your grant application to your...your work, or even the successful use of your preliminary data to secure further funding. Make it clear...

  9. Tips for arabidopsis transformation

    Type
    Blog Post
    ...favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway vectors and can be used for promoter swapping...plants. If your cultures have been incubated at stationary phase for too long, the cells in your culture...4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation ...

  10. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...; multiple codons encode single amino acids. Evolutionary constraints have molded which codons are used...express well in any organism. So what are the evolutionary constraints that lead to these preferences and...6. Pechmann, Sebastian, and Judith Frydman. "Evolutionary conservation of codon optimality reveals hidden...

  11. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...organismal systems described at this meeting were extraordinarily diverse. Plans to develop tools to study their...electric organs arose in different branches of the evolutionary tree at least 6-7 times and on at least 2 separate... Milkweed plants are known to many as the only Monarch butterfly food. They have chemical pathways generating...

  12. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...Crystal structures of CRBNmidi alone (A) and in a ternary complex with the multiple myeloma drug mezigdomide...CRBNmidi on its own, with drug ligands, and in ternary complexes with other proteins, establishing CRBNmidi...low-expression proteins of interest) and mScarlet-I3 for scenarios requiring rapid maturation and prolonged imaging...

  13. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...Nobody EVER complains that a seminar was too easy to understand. Make your seminar accessible. Let the experts...proper consideration. 3. Present an easy to follow seminar See item 2. Attendees will include grad students...

  14. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...Other tools are designed to be activated within a narrower band of wavelengths to allow multiple tools, such... R., Gunaydin, L. A., Hyun, M., Fenno, L. E., Gradinaru, V., Yizhar, O., & Deisseroth, K. (2012). Principles...

  15. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...to detect the presence or absence of a protein (Renart et al., 1979, Towbin et al., 1979, Burnett et al...from Addgene that you can make on your own.  To narrow your search, first pay attention to the validated...–939 . https://doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser J, Stark GR (1979) Transfer of proteins...

  16. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...communication. Science in the News hosts a podcast, seminars, a blog, conferences, and more for non-scientists...Jessica Welch Have Had to Explain Importance of Narrative in Science Communication Conversation in Science...

  17. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...offering an AAV2 CAG-driven tTA expresser from the Gradinaru lab. This is useful for both in vitro and in vivo...serotypes pAAV-CAG-tTA AAV2 Molecular Tools Gradinaru New viral service pAAV_hSyn1_nLightG AAV9 Biosensors...

  18. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ...senior graduate students, Ben Vincent and Niroshi Senaratne, from the Biological and Biomedical Sciences program...Biology, and he tweets @benjvincent.     Niroshi Senaratne is a graduate of the PhD program in Genetics and...

  19. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...series, we continue our conversation with Niroshi Senaratne and Ben Vincent from the Biological and Biomedical...Biology, and he tweets @benjvincent.     Niroshi Senaratne is a graduate of the PhD program in Genetics and...

  20. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ... timely scientific topics, as well as archived seminar presentations. One of SITN's newest initiatives... things up with a combination of shorter, more narrowly focused lectures, interactive panels, and a student...
Showing: 61 - 80 of 281 results