We narrowed to 92 results for: plasmids pcas

...Find CRISPR Screening Libraries Browse All CRISPR Plasmids ...used for each gene. At the moment, 13 full genome SpCas9 knockout libraries designed by different academic...Library, for example related to strategies beyond SpCas9, or species not currently included in Green Listed...libraries. As of now I have focused on human and mouse SpCas9 knockout libraries, but there are of course great...

...Tools Read other CRISPR blog posts Find CRISPR Plasmids at Addgene  ... for reduced off target editing, such as SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options...

...Check out CRISPR Topic Page Find CRISPR Nickase Plasmids Browse the CRISPR Guide ...nickase experiment Overview of Cas9 nickase We’ll use SpCas9 nickases as examples for this post. The D10A mutation...

...Genome Edit Resources on Addgene.org Find CRISPR Plasmids for Your Research Check out Our CRISPR Guide Find...regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ...

...Website Browse Our Collection of Optogenetics Plasmids Check out Our Genome Engineering Resources Brush...technique was applied to create photoswitchable spCas9, dCas9, and S. aureus Cas9 (saCas9) proteins, showing...

...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described...sgRNA backbone for cloning new targeted plasmids. The plasmid can be digested using SapI, which will reveal...2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice...we have a plasmid expressing SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n...

...Browse Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S. pyogenes Cas9 (SpCas9) is the...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Figure 2: Overview ...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...likelihood that any cell containing the CRISPR plasmid will have all desired genomic edits carried out...genetic loci by cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double...later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease...

...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE... Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning... (Cre recombinase) PDF, 105 KB Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators... proteins pFETCh_Donor ; additional HDR & gRNA plasmids are available for CREB1, ATF1, GABPA, and RAD21...

...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...TALE Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... TALE plasmids. Genome... Genome Engineering TALENs TALEN Plasmids and Kits You may also like... Addgene's Genome...Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering...

...Fluorescent Protein Collection Luciferase Plasmid Collection Promega Plasmid Collection Botman-Teusink Yeast Fluorescent...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene... Plasmid Collections Fluorescent Proteins Empty Backbones Fluorescent Proteins: Empty...Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 424 4 3.0 Prone to dimerization Sirius-N1...Emission (nm) Brightness pKa Maturation Structure Plasmids Aquamarine 420 474 23 3.3 Prone to dimerization...Emission (nm) Brightness pKa Maturation Structure Plasmids AcGFP1 475 505 28 Prone to dimerization AcGFP1...

...empty gRNA plasmids . For more information on CRISPR plasmids and function, see our CRISPR plasmids resource...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive...processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert... Browse our list of available viruses based on plasmids in our repository that express fluorescent proteins...-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting 19,114 human...non-targeting controls. This backbone does not contain SpCas9. 76,441 unique sgRNAs targeting 19,114 human genes...non-targeting controls. This backbone does not contain SpCas9. 78,637 unique sgRNAs targeting 19,674 mouse genes...

...Khakh Discontinued Penn Vector Core Viral Vectors (Plasmids still available, other serotypes may be available...available, click on the links for more information. Plasmids are available for all the items listed below. ...pAAV.CMV.Luc.IRES.EGFP.SV40 James M. Wilson AV-9-PV1262 Plasmid not received AV-9-PV1522 100056-AAV9 pAAV.EF1a.....ChR2-YFP.WPRE.hGH Karl Deisseroth AV-9-PV1814 Plasmid not received AV-9-PV2055 105546-AAV9 pENN.AAV.CB7...ALL864 51503-AAV1 AAV pCAG-FLEX-tdTomato-WPRE Hongkui Zeng AV-9-ALL864 51503-AAV9 AAV pCAG-FLEX-tdTomato-WPRE...Control Karl Deisseroth AV-1-ALL854 51502-AAV1 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng AV-1-PV0102 ...Control Karl Deisseroth AV-2-ALL854 51502-AAV2 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng AV-2-PV0101 ...

...Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a large collection...Browse all Neurodegeneration Plasmids Do you have suggestions for other plasmids that should be added to this...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...collection of neurodegeneration plasmids for research related to Alzheimer's Disease (AD), Amyotrophic...others. The table below can be sorted by disease, plasmid type, mutations, and/or tags such as fluorescent...particular gene of interest, please search our full plasmid collection using the search bar at the top of this...Tags GFP FP other Flag GST His TEV Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral...

...posts , or find CRISPR plasmids for your research. Addgene has CRISPR plasmids for the following models... in the field, and enabling access to multiple plasmids used for various platform applications. For more...Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very low off-target...Researchers have expanded the CRISPR field beyond SpCas9, moving us closer to being able to target every... Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs...

...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor.... Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms...individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Tagging Plasmid: 3xFLAG-2xSTREP Construct for integrating at the AAVS1 "safe harbor" locus: eSpCas9(1.1)_...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...

...Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids Packaging Plasmids Envelope Plasmids Additional...packaging plasmid. Bob Weinberg 12259 pMD2.G VSV-G-expressing envelope plasmid. Didier Trono 35617 pCAG-Eco ...envelope plasmid. Arthur Nienhuis and Patrick Salmon 35616 pCAG-VSVG VSV-G-expressing envelope plasmid. Arthur...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...particles have been split into multiple plasmids for safety: Transfer plasmid — contains transgene, sgRNA, or ...flanked by LTRs Packaging plasmid — contains packaging genes Envelope plasmid — contains packaging gene...Popular Gamma-Retroviral Transfer Plasmids Want a retroviral plasmid with your gene of interest? Search...

... Richard Kahn Lab Plasmids GTPase Plasmids GAP and GEF Plasmids References The ARF (ADP-ribosylation factor...examples of plasmid types available for each gene. Click the gene name for a full list of plasmids available...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs. Plasmid... Plasmid Collections...Collections ARF Superfamily Plasmids for ARF Superfamily Research You may also like... RAS Clone Collections Richard...

... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Transfer Plasmids Packaging ...Packaging Plasmids Envelope Plasmids Resources Lentiviruses, a subset of retroviruses, are some of the most common...(a virion) are divided among multiple plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA...flanked by LTRs Packaging plasmid — contains packaging and regulatory genes; one plasmid in second-generation...second-generation, two plasmids in third-generation Envelope plasmid — contains packaging gene env This collection highlights...Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express a CRISPR gRNA? Browse...catalog for lentiviral CRISPR plasmids for gRNA expression . ID Plasmid Generation Description PI 8453...

...Resources Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research Plasmids Blog: Inducible Promoters.... ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for ...with puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection...search our collection for all dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail..., 2017 ) for other viral tTA plasmids. tTA Viviana Gradinaru 104102 pCAG-tTA Mammalian expression of tTA...expression and find plasmids for your experiments using tet- or dox-inducible systems. Plasmid...

...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization... Plasmid Collections Fluorescent Proteins Subcellular Localization...to a red fluorescent protein like mCherry (find plasmids for cloning in our Fluorescent Proteins Empty ...general cellular organelles and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein...below to find markers of cytoskeletal elements. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein...below to find markers for cellular junctions. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein...