We narrowed to 129 results for: sacs

1. The Golden Stopwatch Award: Recognizing Facilitators of Research

   Type

   Blog Post

   ...University of California, Irvine University of Massachusetts, Worcester  University of Nebraska Medical Center...

2. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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   Blog Post

   ...like GFP, some smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest. Promoter...

3. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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   Blog Post

   ...length, NgAgo is shorter than SpCas9 (1368 aa), SaCas9 (1053 aa), or Cpf1 (1228 or 1307 aa depending on...

4. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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   Blog Post

   ...PMC1209583. Watanabe, Tsutomu, Chizuko Ogata, and Sachiko Sato. “EPISOME-MEDIATED TRANSFER OF DRUG RESISTANCE...

5. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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   Blog Post

   ... various metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques that are easily...

6. TALEN Guide

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   Collection

   ...Addgene’s history. Dr. Keith Joung’s lab at Massachusetts General Hospital also recently released a TALEN...

7. Lentiviral Prep Service

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   Collection

   ...Catalytically-dead Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator . ID...

8. New and Upcoming Viral Vectors - June 2019

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   Blog Post

   ...ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120  AAV1 pAAV-ihSyn1...

9. Visualizing Genomic Loci with CRISPR-Sirius

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   Blog Post

   ..., Thoru Pederson’s lab at the University of Massachusetts Medical School developed a dCas9-based multicolor...

10. Improving Transduction Efficiency with Exosome AAVs

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    Blog Post

    ... In 2012, Casey Maguire and colleagues from Massachusetts General Hospital and Harvard Medical School ...

11. K. phaffii: Rising to the Occasion

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    Blog Post

    ...Resources References Liu, Wei, et al., From Saccharomyces cerevisiae to human: The important gene co‑expression...

12. Cultivating Community Science at BosLab

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    Blog Post

    ...as a nonprofit organization in the state of Massachusetts. Because we’re conveniently located next to ...

13. Components of CRISPR/Cas9

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    Blog Post

    ... SpCas9-NG 3' NG  Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N)  Acidaminococcus sp. (AsCpf1...

14. Plasmids 101: Inducible Promoters

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    Blog Post

    ...activator rtTA (reverse tetracycline-controlled transactivator) is normally inactive and cannot bind the tetracycline...

15. A Deep Dive into BioBuilder

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    Blog Post

    ...High School Apprenticeship, preparing local (Massachusetts), economically disadvantaged students for paid...

16. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post

    ... crRNACpf1 Endonuclease size spCas9: ~4 kbsaCas9: ~3 kb ~3.9kb crRNA/gRNA length gRNA: ~100 ...

17. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post

    ...fruit juice clarification, meat tendering, and oligosaccharide synthesis. A major incentive to use cold-active...

18. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    ...’s Association (HBA) Boston Chapter and the Massachusetts Association for Women in Science (AWIS) mentoring...

19. Addgene: The First Twenty Years

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    Blog Post

    ...approvals. In 2013 we were thrilled to receive the Massachusetts Nonprofit Network Excellence Award for our electronic...

20. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    ...or hPR ligand binding domains and the MSN2 transactivation domain. Transcription factor activation via...