We narrowed to 94 results for: sgrna

...3xFLAG-tag fusion proteins. The repair template and Cas9-sgRNA plasmid is then injected into the germline of young...

...impact of off-target mutations, scientists are using sgRNA for selection and optimization. In addition, the...

...104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA TetR H1-2O2 Nathanael...

... predicting CGBE editing outcomes and assisting sgRNA selection is available at http://www.sunlab.fun:... Acronym Full name Notes CRISPR terms gRNA/sgRNA Guide RNA RNA used by Cas9 to locate a matching ...

...γ-Retroviral transfer plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is...

...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34(5) (2016...

...specific RNAs, such as Cre mRNA or Cas9 mRNA and sgRNA, in human and mouse cells. They further develop ...

...genomic locus in any cell type. By varying which sgRNAs are used with FIRE-Cas9, experiments can be designed...

...PMC4151723. 2. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target...

...using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins." Nature communications 10.1 ...

...Nicotiana benthamiana . Plasmids for expressing sgRNA and Cas9 in rice . Includes a protocol (Link opens...

...SapI yields 2 sites- the first site accepts the sgRNA target sequence for U6 promoter expression and the...

...(SAM) Description SAM uses specially engineered sgRNAs to increase transcription. This is done through...

...systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme...using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target site by...efficiency PE3 — increased efficiency; an additional sgRNA directs Cas9n to a nearby site, driving mismatch...in nature and is also commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the ... D. E. (2014). Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation. Nature...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nature Biotechnology . 34 (...

... (Yu et al., Nat Chem Biol). Mutate the PAM or sgRNA sequences Cas9 likely goes through multiple rounds...

...an expression cassette for the Cas9-programming sgRNA and HR donors for selectable genome modification...

...plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging ...

... as WU-CRISPR (Wong et al., 2015)) or our lab’s sgRNA scorer 1.0 (Now 2.0) (Chari et al., 2015) and picking...

...systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme...using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target site by...efficiency PE3 — increased efficiency; an additional sgRNA directs Cas9n to a nearby site, driving mismatch...in nature and is also commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the ... D. E. (2014). Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation. Nature...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nature Biotechnology . 34 (...

...The library was cloned into the Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses...that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides against...obstacle. With CRISPR-X, dCas9 is used to recruit an sgRNA with MS2 hairpin binding sites, which then recruit...