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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 76 results for: tet off

Showing: 61 - 76 of 76 results

  1. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker KanR....early embryogenesis (Figure 3). mScarlet-I3 also offered clearer signals due to reduced background signal...

  2. Deisseroth INTRSECT Collection

    Type
    Collection
    ... 55651 pAAV-hSyn Con/Foff EYFP Cre AND NOT Flp F3/F5 No 137162 pAAV-Ef1a-Con/Foff 2.0-EYFP Cre AND NOT.../F5 Yes 55652 pAAV-hSyn Coff/Fon EYFP Flp AND NOT Cre No 231927 pAAV-Ef1a-Coff/Fon-EYFP Flp AND NOT Cre...137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT Flp FRT/F5 Yes 137131 pAAV-Ef1a-Coff/Fon-BFP Flp AND NOT ...137133 pAAV-Ef1a-Con/Foff 2.0-mCherry Cre AND NOT Flp FRT/F5 Yes 137134 pAAV-Ef1a-Coff/Fon-mCherry Flp AND...137137 pAAV-Ef1a-Con/Foff 2.0-oScarlet Cre AND NOT Flp FRT/F5 Yes 137138 pAAV-Ef1a-Coff/Fon-oScarlet Flp ...137120 pAAV-Ef1a-Con/Foff 2.0-GCaMP6M Cre AND NOT Flp FRT/F5 Yes 137121 pAAV-Ef1a-Coff/Fon-GCaMP6M Flp AND...137123 pAAV-Ef1a-Con/Foff 2.0-GCaMP6F Cre AND NOT Flp FRT/F5 Yes 137124 pAAV-Ef1a-Coff/Fon-GCaMP6F Flp AND...

  3. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...excited to offer these useful tools to the community. Among other new biosensor tools, we now offer the jGCaMP7c...To help make this easier for scientists, we now offer trial size 20 µL samples of control AAV that scientists...control of specific neuronal populations. We now offer a Flp-expressing AAV, as well as a Cre-dependent...  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120...

  4. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...organization’s technology transfer office or other authorized office directly to make sure the deposit ...nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of the above, or other. High...to Addgene and contact your technology transfer office about the MTA. Pro Tip! Download a new spreadsheet...

  5. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...distributes to per year, 2005-2023. We identified and offered a number of solutions that increased accessibility...each courier we contracted with so we can always offer the fastest shipping. However, there were issues...specific to that country. ‌For example, when we began offering temperature-sensitive materials, we noticed a ...Culture Collections Lentiviral Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial ...educational resources for each type of resource we offer.  In 2013, we launched the Addgene blog for topics...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...188572 Tet-off TauRD (P301L/V337M) MAPT Myc TRE Parkinson's, FTD Franz-Ulrich Hartl 188573 Tet-off Full ...Ataxia Richard Youle 176485 Piggybac-TetO-hNurr1-blast NR4A2 TetO Parkinson's Marius Wernig 176852 UBQLN2...Ronald Hart 190811 pTet-O-APOE-E2-T2A-Puro APOE UbC Alzheimer's Ronald Hart 190812 pTet-O-APOE-E3-T2A-Puro...Flag, GFP CMV ALS Tanja Mittag 234848 pLV.tetO.Nurr1 NR4A2 TetON Parkinson's Malin Parmar 234872 PGK-HA-PGRN... CMV Ataxia telangiectasia Stephen Elledge 43918 tetO-ALN NR4A2 His, V5 TRE Parkinson's John Gearhart ...T2A-Puro APOE UbC Alzheimer's Ronald Hart 190813 pTet-O-APOE-E4-T2A-Puro APOE UbC Alzheimer's Ronald Hart ...Lukas Trantirek 232478 pB-TR-hSOD1 SOD1 Flag CMV-TetO ALS Lukas Trantirek 232481 pHLsec hSOD1 SOD1 Flag...

  8. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...institutes that offer shared equipment that you can use at a lower cost. Don't tether yourself to a lab...on unnecessary materials, or even a loss of the offer all together. The problem is that the discussions...turned down a faculty position because none of the offering institutes were able to provide the resources ...

  9. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...for up to 60% of the cell culture contamination (Uphoff, 2002). Mycoplasmas are considered to be one of...for at least two or three days before sampling (Uphoff and Drexler, 2011). Culturing on agar plates/broth...fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline, etc. are available for mycoplasma removal from... of mycoplasma species. J Microbiol .44: 42–49  Uphoff Cc, Drexler Hg (2002) Comparative Antibiotic Eradication...10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting Mycoplasma Contamination...

  10. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...professional development and networking resources offered by programs like IMP USA-Europe not only provide...University, the Salk Institute, and the Karolinska Institutet, among others). Mentors and mentees held scheduled...for the Spanish scientific community overseas. Official representatives from different Spanish universities...

  11. Lentiviral Vector Guide

    Type
    Guide
    ...to the growth medium of the host cells, they kill off any cells that have not incorporated the lentiviral...LEDGF (also known as PSIP1 or p75 ), a lentiviral tethering gene that helps facilitate integration. Despite... of experiments being performed. Your biosafety office can provide more information on your institution's...PMID: 32502836 Johnson, N. M., Alvarado, A. F., Moffatt, T. N., Edavettal, J. M., Swaminathan, T. A., &...30518911 Wells, D. W., Guo, S., Shao, W., Bale, M. J., Coffin, J. M., Hughes, S. H., & Wu, X. (2020). An analytical...

  12. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...transferable skills while working in the lab and by offering advice on areas where you might need some help...collaborations. During my graduate studies at Karolinska Institutet I started working with other groups. First within...

  13. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...multimeric forms (e.g. concatemers, dimers, trimers, tetramers). Since then, multimerization has been observed...-340-4_2. PMID: 19763938. Maucksch C, Bohla A, Hoffmann F, Schleef M, Aneja MK, Elfinger M, Hartl D, Rudolph...

  15. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...uptake or intracellular transport machinery. Addgene offers viral preparations of AAV2-retro vectors with a...retrograde transsynaptic transfer of a protein, tetanus toxin, subsequent to its retrograde axonal transport...

  16. Molecular Biology Reference

    Type
    Guide
    ... a reporter gene (e.g., luciferase or GFP) that offers a readout of the activity of the genetic element...England Biolabs F' proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR) ∆(ara-leu) 7697 araD139 fhuA ∆lacX74 galK16 galE15.../mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg/mL *Carbenicillin can be used...
Showing: 61 - 76 of 76 results