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  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...functional knock-out via frameshift and minimize off-target effects. The library was cloned into the Liu...inserting OsTIR1 into the "safe harbor" AAVS1 locus (a tet-inducible OsTIR1 plasmid is also available). Second...actin 5C promoter. Addgene’s fly library web page offers more details and protocols, and the Liu lab’s OXfCRISPR...unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many of which ...context-dependent human fitness genes, the laboratory of Jason Moffat at the University of Toronto has created the Toronto...demonstrate the effectiveness of their library design, Moffat laboratory members conducted multiple screens in...hits. This work has been published in Cell. The Moffat laboratory maintains a website for the TKO library...

  2. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9." Nature biotechnology...CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. It can be very time consuming to ...CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. He has a particular interest in using...

  3. Genetic Code Expansion

    Type
    Collection
    ...are several things to consider. If you are working off of a previously established protocol, make sure to...Ryan Mehl 85496 pDule-Tet2.0 Tetrazine2.0 tRNA synthetase M. jannaschii Tetrazine 2.0 Bacterial TAG Ryan...Ryan Mehl 85497 pDule2-Tet2.0 Tetrazine2.0 tRNA synthetas M. jannaschii Tetrazine 2.0 Bacterial TAG Ryan...Bacterial TAG Ryan Mehl 164580 pUltraI-Tet3.0[TAA] Tet3.0RS M. barkeri Tet3.0 Bacterial TAA Ryan Mehl 172482 ...TAG Ryan Mehl 174080 pDule-Tet3.0 Tet3.0 tRNA synthetase M. barkeri Tetrazine 3.0 Bacterial TAG Ryan Mehl...NES-Flag-R2-84-MbRS Tetrazine3.0 NES-Flag-R2-84 tRNA synthetase M. barkeri Tetrazine 3.0 Mammalian Ryan...aminoacyl-tRNA synthetase M. jannaschii 1,2,4,5-tetrazine ncAAs Bacterial TAG Ryan Mehl 217361 pIDTSmart-MbPylRS...

  4. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... tension sensor modules Grashoff Lab Tension Sensor Plasmids Carsten Grashoff Tension tCRMod tension sensors...the plasmids associated with the article. We also offer ready-to-use AAV preparations of select plasmids...sensors, ER-targeted CEPIA1er ER-mitochondria tethering by PDZD8 regulates Ca(2+) dynamics in mammalian..., reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM A fluorescent, reagentless ...reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM. ACS Chem Biol. 2010 Apr 16;5(4):415-25...single cells. Angew Chem Int Ed Engl. 2018 Jun 27. Tetsuya Kitaguchi ATP Variants of ATP sensor iATPSnFR1.0...dual-color imaging. PLoS One. 2014 Jun 24;9(6):e100252. Tetsuya Kitaguchi cAMP (cyclic AMP) Red fluorescent protein-based...

  5. MXS Chaining

    Type
    Blog Post
    ... fluorescent protein were fused in-frame to a tethering partner, which directed localization of the fluorophore...Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular localization 1 TagBFP 399nm...suited for particular downstream applications. The tradeoffs between various cloning methods are listed in ...

  6. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker KanR....early embryogenesis (Figure 3). mScarlet-I3 also offered clearer signals due to reduced background signal...

  7. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...excited to offer these useful tools to the community. Among other new biosensor tools, we now offer the jGCaMP7c...To help make this easier for scientists, we now offer trial size 20 µL samples of control AAV that scientists...control of specific neuronal populations. We now offer a Flp-expressing AAV, as well as a Cre-dependent...  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120...

  8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...organization’s technology transfer office or other authorized office directly to make sure the deposit ...nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of the above, or other. High...to Addgene and contact your technology transfer office about the MTA. Pro Tip! Download a new spreadsheet...

  9. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...distributes to per year, 2005-2023. We identified and offered a number of solutions that increased accessibility...each courier we contracted with so we can always offer the fastest shipping. However, there were issues...specific to that country. ‌For example, when we began offering temperature-sensitive materials, we noticed a ...Culture Collections Lentiviral Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial ...educational resources for each type of resource we offer.  In 2013, we launched the Addgene blog for topics...

  11. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...institutes that offer shared equipment that you can use at a lower cost. Don't tether yourself to a lab...on unnecessary materials, or even a loss of the offer all together. The problem is that the discussions...turned down a faculty position because none of the offering institutes were able to provide the resources ...

  12. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...for up to 60% of the cell culture contamination (Uphoff, 2002). Mycoplasmas are considered to be one of...for at least two or three days before sampling (Uphoff and Drexler, 2011). Culturing on agar plates/broth...fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline, etc. are available for mycoplasma removal from... of mycoplasma species. J Microbiol .44: 42–49  Uphoff Cc, Drexler Hg (2002) Comparative Antibiotic Eradication...10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting Mycoplasma Contamination...

  13. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Flp 55651 pAAV-hSyn Con/Foff EYFP Cre AND NOT Flp F3/F5 137162 pAAV-Ef1a-Con/Foff 2.0-EYFP Cre AND NOT Flp...Flp FRT/F5 55652 pAAV-hSyn Coff/Fon EYFP Flp AND NOT Cre 231927 pAAV-Ef1a-Coff/Fon-EYFP Flp AND NOT Cre ... 137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT Flp FRT/F5 137131 pAAV-Ef1a-Coff/Fon-BFP Flp AND NOT Cre...137133 pAAV-Ef1a-Con/Foff 2.0-mCherry Cre AND NOT Flp FRT/F5 137134 pAAV-Ef1a-Coff/Fon-mCherry Flp AND ...137137 pAAV-Ef1a-Con/Foff 2.0-oScarlet Cre AND NOT Flp FRT/F5 137138 pAAV-Ef1a-Coff/Fon-oScarlet Flp AND...137120 pAAV-Ef1a-Con/Foff 2.0-GCaMP6M Cre AND NOT Flp FRT/F5 137121 pAAV-Ef1a-Coff/Fon-GCaMP6M Flp AND ...137123 pAAV-Ef1a-Con/Foff 2.0-GCaMP6F Cre AND NOT Flp FRT/F5 137124 pAAV-Ef1a-Coff/Fon-GCaMP6F Flp AND ...

  14. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...professional development and networking resources offered by programs like IMP USA-Europe not only provide...University, the Salk Institute, and the Karolinska Institutet, among others). Mentors and mentees held scheduled...for the Spanish scientific community overseas. Official representatives from different Spanish universities...

  15. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...188572 Tet-off TauRD (P301L/V337M) MAPT Myc TRE Parkinson's, FTD Franz-Ulrich Hartl 188573 Tet-off Full ...Ataxia Richard Youle 176485 Piggybac-TetO-hNurr1-blast NR4A2 TetO Parkinson's Marius Wernig 176852 UBQLN2...Ronald Hart 190811 pTet-O-APOE-E2-T2A-Puro APOE UbC Alzheimer's Ronald Hart 190812 pTet-O-APOE-E3-T2A-Puro... CMV Ataxia telangiectasia Stephen Elledge 43918 tetO-ALN NR4A2 His, V5 TRE Parkinson's John Gearhart ...T2A-Puro APOE UbC Alzheimer's Ronald Hart 190813 pTet-O-APOE-E4-T2A-Puro APOE UbC Alzheimer's Ronald Hart ...

  16. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...transferable skills while working in the lab and by offering advice on areas where you might need some help...collaborations. During my graduate studies at Karolinska Institutet I started working with other groups. First within...

  18. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...multimeric forms (e.g. concatemers, dimers, trimers, tetramers). Since then, multimerization has been observed...-340-4_2. PMID: 19763938. Maucksch C, Bohla A, Hoffmann F, Schleef M, Aneja MK, Elfinger M, Hartl D, Rudolph...

  19. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...uptake or intracellular transport machinery. Addgene offers viral preparations of AAV2-retro vectors with a...retrograde transsynaptic transfer of a protein, tetanus toxin, subsequent to its retrograde axonal transport...

  20. Luciferase Plasmid Collection

    Type
    Collection
    ...enchancer regions in Drosopholia Herman Wijnen 126033 pTETRIS-cargo Firefly For use as a reporter of the ability...to find plasmids expressing luciferase. We also offer ready-to-use AAV preparations of select luciferase...
Showing: 61 - 80 of 81 results