We narrowed to 83 results for: tev
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TypeBlog Post...between two gene therapy labs (Brian Kaspar and Steven Gray) and two MECP2 labs (Gail Mandel and Stuart...
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Tips for CRISPR Gene Editing in Mice
TypeBlog Post...OriGene, or you can order them yourself through whatever DNA supply company you currently use. You can ... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post...10.3389/fcell.2022.880107. KRaION potassium sensors Whatever your analyte of interest, it’s important to choose... -
Evolution of Lab Techniques
TypeBlog Post...Central PMCID: PMC389558. 7. Sanger, Frederick, Steven Nicklen, and Alan R. Coulson. "DNA sequencing with... -
CRISPR 101: Off-Target Effects
TypeBlog Post...will be on target. Thus, if you isolate/sequence/whatever your editing event outcome is at a reasonable ... -
A Control for All Seasons
TypeBlog Post...0022155414545224. PubMed Central PMCID: PMC4212362. Alexander, Steve P. H., et al. “Goals and Practicalities of Immunoblotting... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...Split Channels and then Color>Merge Channels into whatever combination of colors you like. Controls and validation... -
CRISPR Plasmids - Prime Edit
TypeCollection...MMLV RT) PuroTK Jacob Giehm Mikkelsen 174038 pU6-tevopreq1-GG-acceptor Mammalian hU6 epegRNA BsaI No mRFP1... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post...your protein of interest will end up linked to whatever molecule you choose (Figure 1). Just express the... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...Biochemistry PhD candidate in the laboratory of Prof Steve Jackson at the Gurdon Institute at the University... -
Zebrafish Plasmid Collection
TypeCollection...expression in zebrafish using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct... -
University of Florida Serotype Testing Panel for the Eye and Brain
TypeCollection...2016 Aug;23(8-9):680-9. PMID: 27115727 Rodriguez-Estevez, et al. 2020. Transduction optimization of AAV ... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post....2016.04.091 Cruse JM, Lewis RE, Wang H, Geziena MT, Steven GE, Lorna J. (2004) ANTIGENS, IMMUNOGENS, VACCINES... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...Central PMCID:PMC2172000. 10.Vorvis, Christina, Steven M. Markus, and Wei‐Lih Lee. "Photoactivatable GFP... -
CRISPR Guide
TypeCollection...to dCas9 and co-expressing BirA biotin ligase. Whatever the chosen epitope tag, the locus is subsequently.... PMID: 35236982 Bubeck, F., Hoffmann, M. D., Harteveld, Z., Aschenbrenner, S., Bietz, A., Waldhauer, ... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...the future! References Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Börner... -
Deep Dive: qPCR
TypeBlog Post...created in BioRender. Passive Reference Dyes Whatever your pipetting method, some variation will occur... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post...Gaudelli, N. M., Lam, D. K., Rees, H. A., Solá-Esteves, N. M., Barrera, L. A., Born, D. A., Edwards, A... -
Technical Design of a Western Blot
TypeBlog Post...C, and are very consistent gel-to-gel. Ladder Whatever gel type you select, don’t forget to save a lane... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...or place a double ladder on the lefthand side. Whatever you do, be consistent and document it. Did it...