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We narrowed to 68 results for: tev

Showing: 1 - 20 of 68 results

  1. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...of the TEV cleavage and improve utility of the system for use with low affinity variants of TEV. The new...evolution platform to generate TEV protease variants. Catalysis by TEV protease allows a transcription...catalytic efficiency of tobacco etch virus protease (TEV), a highly sequence-specific protease used to cleave...

  2. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...best optimized is the tobacco etch virus (TEV) protease. A TEV protease cleavage site is frequently placed...comprised of a calmodulin binding peptide (CBP), a TEV cleavage site (more on that in a moment), and 2 ProtA...cleavage reaction taking place between column runs. The TEV protease itself, with various mutations used to increase...with tags Protease Recognition site Notes TEV ENLYFQS Cleaves between the Gln and Ser residues ...

  3. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...few of our proteins that lacked TEV. Although we were unable to cleave TEV within the protein we tested,...the AviTag. As a side note, we found that adding a TEV linker dramatically increased accessibility of the...

  7. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...with each possible combination of protease site (TEV or PreScission), epitope tag (12xHis, 2xStrep, 3xFlag...

  9. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...additional useful tags, including 3xFLAG epitopes, TEV and BioTag, which can be used in protein affinity...

  11. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ... knock-out in your experiments? AAV-flex-taCasp3-TEVp from Nirao Shah's and Jim Well's lab induces cre-dependent...viral prep in serotype AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting...

  12. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...are then washed, followed by antibody elution. Whatever method you choose, you may not be able to elute...time, reagents, and equipment available to you. Whatever you choose, you’ll want an accurate concentration...

  13. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...and Chemistry activities from Quatr.us Foldscopes Steve Spangler Science The Fab Lab Middle school: supplementing...exciting, tumultuous time in scientific history. Whatever your reasons, let us know how it goes! Acknowledgements...

  14. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...the transgenic genome will simply express from whatever promoters were included in the vector (or are ...instead get expression of the sensor, marker, or whatever other tool you chose to express. In the case ...

  15. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...there. Make sure you read the instructions for whatever antibiotic you choose to ensure it works in your...molecular language as the tree outside my window! Whatever you use them for, shuttle vectors can help you...

  16. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ..., you can easily convert the citation style to whatever the journal requires. Using the (Last name, Year...woods for months at a time, so take advantage of whatever time you have. If you’re like me, you might be...

  19. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...Meltzer, J. C., Buzhdygan, T., Cheng, M. J., Sena-Esteves, M., Bennett, R. E., Sullivan, T. P., Razmpour,..., Allaway, K., Sakopoulos, S., Schneider, G., Stevenson, O., Vergara, J., Sharma, J., Zhang, Q., Franken...
Showing: 1 - 20 of 68 results