...Oleispira antarctica, and the P. haloplanktis Trigger Factor), that would allow the stable folding and expression...compared to the E. coli strain expressing the empty vector. And thus was born ELSA - our happy lil’ cold-tolerant...of stakeholders from industrial and commercial operators, government agencies, active researchers, as well...interact with personnel from the industry and brainstorm with each other. The event also featured a seminar...The talk highlighted the importance of reagent repositories and the myriad facilities offered by them which...understanding of the societal ramifications of laboratory science, and how it may best be leveraged for...re always delighted to engage with potential collaborators and interested audiences. Please visit our team...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...regulate botulinum neurotoxin were also created. The depositors demonstrated the utility of light-mediated suppression...slices from mouse, and showed that it can alter motor responses in C. elegans. Find the split BoTN/B plasmids...acoustic reporter gene (ARG) plasmids to better monitor gene expression in vivo using ultrasound. These.... 2018. PubMed PMID: 29300010. miRNA reporter vectors shed new light on mechanisms of miRNA-mediated ...microRNAs (miRNAs) are recognized as important regulators of post-transcriptional gene expression across...They designed the pXPR_053 lentiviral expression vector, which contains an sgRNA insert site and a reporter...

...as several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used yeast display-...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...Recently the Vellai lab has created an improved RNAi vector that leads to significantly stronger phenotypes...for mutant genetic backgrounds. The standard RNAi vector, L4440, places a target gene between two inverted...Vellai lab’s new plasmid, T444T, contains T7 terminators adjacent to the T7 promoters to prevent the incorporation...Recently, the Sidhu group at the University of Toronto expanded the specificity landscape of the SH3 proteins...

...This will be highly dependent on vector dose. The color diversity in Vector Assisted Spectral Tracing (VAST...catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may...drop in transduction coverage compared to just one vector.   Q2: Do you think it is possible to make an efficient...this scenario, you may be best served by a dual vector, split intein approach.   Q3: What’s the best method...concentrated to ~1x1014 vg/ml.. As evident in our repository, this application is an outlier for PHP.eB (1x1011...Plasmids Adenovirus Guide Page Subscribe to Viral Vectors Blog Posts ...

...her then was this: Can you engineer CRISPR-Cas9 activators that work well enough on any gene that they could...which they call CRISPR/Cas9 Synergistic Activation Mediator or SAM. SAM is an engineered protein complex for...information from the structure to guide and improve activator design,” she said. “Before it was a little like...Konermann recognized as an ideal spot to fuse the activators instead of at the protein’s C terminus. With ...activation complex consisting of multiple distinct effector domains that work together synergistically, modeled...melanoma cells resistant to treatment with a BRAF inhibitor. (The test was the perfect complement for one ...repressing or inducing individual transcripts. The activator (CRISPRa) sgRNA library uses the sunCas9 system...

...viral vector samples were distributed in 2018 and we are poised for more offerings. The viral vector team...don’t have worry or wonder if providing a viral vector service was a good idea. The scientific community...don’t have to worry about getting support from depositors. We regularly hear how much they see the value... 3.5 Addgenies strong. The Addgene Board of Directors is mature and poised to support our growth. Thanks...Addgene is about half of what is standard in most sectors. The Addgenies support a high collaboration, high...blog Subscribe to the Addgene blog Read our viral vector blog posts Learn more about Addgene Resources ...

...via the secretory pathway, and then cleaved to generate an active form of the protein. To monitor autophagy... Mark Prescott. This system was developed for monitoring and analyzing autophagy of cytosol and organelles... samples treated with or without an autophagy inhibitor. While this method does provide a quantitative...the mammalian Atg8p homolog, can also be used to monitor autophagy, but under some conditions it aggregates...also possible to make Rosella fusions and thereby monitor autophagy of your protein of interest. Are you ...Cuervo, A. M., & Seglen, P. O. (2007). Methods for Monitoring Autophagy from Yeast to Human. Autophagy,3(3)...

...contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer Institute. Targeted...provide distinct advantages over pharmacological inhibitors including rapidly depleting a protein-of-interest... target selectivity, overcoming resistance to inhibitors, and inducing prolonged biological effects. ...et al., Nature Chemical Biology for examples). Monitor neo-substrates Small molecule degraders may recruit...and IZKF3 are cell-type specific transcription factors with important roles in Multiple Myeloma), it is...studies (Nabet et al.).   Behnam Nabet, PhD is a postdoctoral fellow at the Dana-Farber Cancer Institute. ...dTAG-47 Weintraub et al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 ...

... collisions you may encounter: vector, sequence, insert, trait, factor, expression, etc.There are also... Laboratory management software is not a requisite for a functioning lab, but it is for a scaleable lab...intentionally trivial, but they're here to help tell this story. In this first example to the right (# this will...about what steps are most likely to fail. Our laboratory manager would definitely be interested to see...this case, the physical considerations of the laboratory make object composition a smarter choice than...terms (plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you simply don't have enough...

...particular surface receptor are stained with a fluorescent antibody against the receptor and analyzed on ...fluorochrome is removed from all other detectors except its dedicated detector. To ensure proper compensation ...directions. The degree of scatter is captured by detectors and provides useful information about the cell...emits a fluorescent signal that is captured by detectors. The strength of the emitted photons varies from...to further separate the B cell population into progenitor B cells, immature B cells, plasma cells or others...flow cytometry experiment depends on a variety of factors including sample preparation, staining procedures...