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We narrowed to 971 results for: lars;

Showing: 391 - 400 of 971 results

  1. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...commonly used to create functional knockouts or large deletions, the ability to analyze and interpret ...which of the multiplexed sgRNA was involved in a particular edit, and which type of edit was produced.   ...characterizes the sequence and abundance of each particular indel.   For a complete guide to ICE analysis...Typically, a yellow check mark indicates that a particular parameter was automatically adjusted to generate...+F or Command+F functionality to search for a particular sequence or name. You can perform more in-depth...Contributions, Indel Distribution and Traces - provides particular details about the indel profile of the edited...

  2. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...two independent groups, led by Jack Szostak and Larry Gold, developed methods for selecting and amplifying...metal ion, or fold to incorporate themselves into a larger target, such as a protein. Such flexibility makes... Design and selection for targets can be done largely in vitro; Easy and reproducible commercial synthesis...advantages of oligonucleotides over proteins is their modularity, which is lost as soon as the aptamer is bound... Assays that require recognition of ions or particularly small molecules that do not generate an immune...Central PMCID: PMC4071757. 6. Hong, He, et al. “Molecular Imaging with Nucleic Acid Aptamers.” Curr Med ...

  3. How to Write a Scientific Review Article

    Type
    Blog Post
    ..., to search the literature, and to synthesize a large volume of information: basically, a scientist’s ...colleague, or your adviser was invited to write on a particular topic. Alternatively, maybe you’re researching...helps you later on to remember where you read particular studies or experiments. Later, you can easily... searched for recent research papers on these particular themes, for seminal papers on these themes, and...also did not limit myself to the well-cited or popular papers, but looked for papers from a wide range...present it all in one place to get a sense of the similarities and differences among proteins in the class....

  4. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...behind the cytotoxicity can vary. For example, the popular VSV-G envelope protein causes cells that it infects... were produced in. For example, production of vesicular stomatitis virus, HIV-2, and human foamy virus...intact SARS-CoV-2 virus. Scientists have been using similar pseudotyping methods with other viruses in a BSL...envelope protein for pseudotyping is VSV-G, or vesicular stomatitis virus G protein. VSV-G is a trimeric...in Human Cells Are Inactivated by Human Serum. Molecular Therapy 2:218–222 . https://doi.org/10.1006/mthe...nervous system after peripheral delivery. Human Molecular Genetics 10:2109–2121 . https://doi.org/10.1093...

  5. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...your science. While Reddit isn’t for everyone, particularly if you’re more interested in selling a product...topic”) which are essentially lists of forums on particular topics. Volunteer moderators set the rules for... and, at least sometimes, civil. r/Science in particular hosts a variety of conversations with people ...can ask designated scientists anything about a particular topic. Given Joanne’s expertise in science careers...Reddit. Here are some reasons why: 1. Reddit has a large and active community of people interested in science... moderators did not delete or suppress were the large number of scientist posters with serious career ...

  6. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...and its family members serve as the cellular receptors for vesicular stomatitis virus.” Proceedings of ... can persist undetected; unlike infections with larger microbes such as yeast, fungi, or bacteria, mycoplasma... medium. Daily medium exchanges help alleviate cellular stresses associated with pH changes and resource...Detection of mycoplasma contaminations.” Methods in Molecular Biology 946 (2013). 1-13. Pubmed PMID: 23179822...lentiviral vectors: effect of sera and polycations.” Molecular Biotechnology 53.3 (2013). 308-14. Pubmed PMID...

  7. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre for Advanced...protein to study its function is one of the most popular applications of fluorescent proteins. These fusion...Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced...Bendezú, Felipe O., et al. "Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based...291.5512 (2001): 2408-2411. PubMed PMID: 11264536. Klarenbeek, Jeffrey, et al. "Fourth-generation epac-based...dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins." Proceedings...

  8. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...IF), is an immunoassay that brings to light the cellular world. The technique allows you to ask questions...secondary antibodies on hand to mix and match with a larger collection of primary antibodies. This strategy...this step as well. Preservation and Imaging Similar to Sample Preparation, this step can involve multiple...sections of papers that have successfully used your particular antibodies and refer to the manufacturer’s recommendations...steps used in a non-fluorescent IHC/ICC assay are similar to the ones you plan to use, then the primary antibody...Performing Immunofluorescence Staining. In: Methods in Molecular Biology. Springer New York, pp 299–311. https:...

  9. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... target to specific subcellular regions, laser ablation can damage other cellular structures, and genetic...activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength...the flip of a switch. You may be familiar with popular light-sensitive ion channels used to control activities...Opto-RhoGEFs to study cell junction integrity in the vascular barrier of the endothelium, but they could be ...

  10. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...lymphocyte population distinct from cellular debris and other larger cells. Note, we don’t mind the warning... scattered by that cell at a low angle (FSC) or large angle (SSC) (Figure 1). FSC values depend on the...forward at a low angle (FSC) or to the side at a large angle (SSC). FSC and SSC depend on the cell’s size...cell population will show up on an FSC/SSC plot, largely depends on cell type and laser voltage. The voltage...populations displayed. As the separation pattern largely stays the same and does not impact my ability to...
Showing: 391 - 400 of 971 results