Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,019 results for: CAL;

Showing: 781 - 800 of 1019 results

  1. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...relationships as part of the job application process can dramatically increase the chances of being seriously considered...you are qualified and why you are interested specifically in the position. We frequently receive clearly...

  5. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...capsid other than AAV2. This production strategy is called cross-packaging.  Capsid (cap) gene The cap gene... of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248–265 . https://doi.org/10.1016...

  6. Fostering communication in remote collaborations

    Type
    Blog Post
    ...virtual face-to-face meetings as opposed to phone calls most closely replicates how you’d interact with ...investigate my protein of interest in a French clinical cohort, neither of which I would have been able...

  7. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...host range and copy number of the plasmid) and typically include a gene that is advantageous for survival...Plasmids 101: Luciferase Monitor the level of a chemical in a particular environment Produce enzymes that...

  8. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...could be used in high throughput screening for agrochemical compounds that have the potential to regulate...and AAVrg vectors. New red-shifted INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments...

  9. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ... (ADCD) Antibodies can bind to a pathogen and physically prevent it from entering a cell, a non-Fc-dependent...antibodies on a pathogen activates the immune system's classical complement pathway, starting a cascade of events...

  10. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...throughout an organism’s genome. These reporters have historically been used extensively in fly, mouse, and yeast.... These are effective tools but neglect some biologically relevant situations. Tissue-specific genes...

  12. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...blogosphere is to read and comment on their blogs. 5)   Local science museums are a great place to find opportunities...Science Communication Program at the University of California, Santa Cruz, has been turning scientists into...

  13. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...How else can antibiotics be used in the lab? Historically, antibiotics have also been used to disrupt ...cloning. In these instances, the cloning vector typically has two separate resistance cassettes and your...

  14. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...(CellTagD13), which is when the iEP phenotype typically emerges. Every three days, a sample of cells was...conversion, but CellTag could be used to study other biological processes where cells are undergoing identity...

  17. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...distribution. I don’t have space in this blog to call out every Addgene Team because they’re all doing...requests for scientists doing almost every type of biomedical science research. Our work accelerates and enhances...

  18. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ... sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ITRs due to their secondary...of lox sites which are part of a dual lox system called FLEX or DIO. Lox sites are recognized by the recombinase...

  19. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...Randolph-Habecker J (2013). A Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free...Tuson JR, Pascoe EW, Jacob DA (1990) A novel immunohistochemical technique for demonstration of specific binding...

  20. Plasmid Cloning by PCR

    Type
    Blog Post
    ...analyzing the gene’s function. The process is shown graphically in the following figure, in which we add EcoRI...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...
Showing: 781 - 800 of 1019 results