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We narrowed to 971 results for: LARS

Showing: 781 - 800 of 971 results

  4. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...systems, methyltransferases, methylate DNA at particular sequences to prevent them from being degraded...Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular Biology Background ...

  6. dTAG - You're it!

    Type
    Blog Post
    ...proteasome machinery to degrade any protein. We are particularly excited about the dTAG system as we can use ...broad utility for degrading targets in different cellular compartments, and in vivo utility. dTAG molecules...

  8. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...assigned a paper to give as a journal club to my molecular biology class. A Cell paper. With six figures....and/or apprehensive. I still get nervous quite regularly, and I've had multiple jobs where all I did was...

  9. To Each HIS Own

    Type
    Blog Post
    ...Moks 1987). These tags, however, tended to be very large and often required the incorporation of a cleavage...M, Enfors SO, Persson I, Nilsson B and Uhlen M. Large-scale affinity purification of human insulin-like...

  10. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...increased sensitivity, polyclonal antibodies are particularly useful for certain research applications. Many...composition available. If you’re going to need a large quantity of antibody for your experiment, you should...

  11. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...edited embryos proceeded to the blastocyst stage at similar rates to untreated controls. Ma et al. performed...germline editing. Although this paper represents a large step forward in the CRISPR field, it’s important...

  12. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...hormone therapy resulted in 76,000 fewer cases of cardiovascular disease, 126,000 fewer breast cancers, and ... Scientific Impact Are Evoloving Check Out Extracurricular Activities for a Strong Science Career Path...

  13. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ... science and tech get jobs. She is also a very popular speaker and workshop leader. Lauren Celano of Propel...Confident and Successful Industry Professionals”. This larger shop might appeal to some although most scientist...

  14. A Look at Addgene's QC Process

    Type
    Blog Post
    ...The raw sequencing reads are assembled into a circularized plasmid sequence de novo (without the use of...pipeline. There are regions of plasmids that are particularly difficult to sequence and assemble using the...

  15. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...biological questions about the brain. To address this, molecular tools to map neuronal connectivity are widely ... Using these technologies in combination with molecular tools (e.g., optogenetic or chemogenetic receptors...

  16. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...there’s no sense in spending valuable research dollars on a new secondary antibody if you don’t need it... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice...

  18. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen lab, now available in a ready to use AAV5 viral...

  19. CRISPR Library Amplification

    Type
    Protocol
    ... Pooled Libraries All CRISPR Pooled Libraries Molecular Biology Reference How do I process my Addgene ...libraries up to 200,000 individual plasmids. For larger libraries scale the number of cells and DNA accordingly... LB Agar + Antibiotic 245 mm bioassay plates (Molecular Devices, X602) Pro-Tip Pour these plates at least...

  20. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7...
Showing: 781 - 800 of 971 results