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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 974 results for: LAT

Showing: 781 - 800 of 974 results

  2. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...to repair the DNA using the edited strand as a template — in this case, encouraging it to incorporate ...Like other transversion editors, this editor had relatively high indel rates ranging from 4.7% to 30% at ...

  3. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...; the importance of which will be elaborated on later. As for what job fairs to consider, some of the ...the way. Make connections, and nurture those relationships. Then when you are ready to apply for your dream...

  4. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...therapeutic drugs, vinblastine and vincristine, are regulated in the medicinal plant, the Catharanthus roseus...investigate transcription factor candidates and the regulation of genes in the biosynthetic pathway. Why plant...

  5. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ... of the Z pathway" is vague and "what proteins regulate the Z pathway?" is an open-ended list of proteins...proteins. Instead, consider "how do proteins X and Y regulate the Z pathway?" A well-defined outcome makes it...

  6. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...feasible, it leaves little room for additional regulatory elements. Feng Zhang's group previously packaged...for Cas9. However, to allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs...

  7. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...and experimental needs. Since its invention in the late 1990s, Gateway cloning technology has become very...Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony PCR to Quickly...

  8. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...session is to start with something completely unrelated to the issue and consider how it could be shaped...problem is to step away from it. Find something unrelated to do (many Addgenies enjoy rock climbing, for...

  9. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...applications, they have become indispensable tools for manipulating cells and organisms. They can be used for a ...This is because most titering methods rely on isolating genomic DNA from transduced cells and quantifying...

  10. Evolution of Lab Techniques

    Type
    Blog Post
    ... require sequencing of many samples at once for later comparison. These next-generation sequencing techniques...technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move ...

  11. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...different opinions, and some of them would acknowledge later that without my “neutral” presence to alleviate ... that nobody is really owning. Conflict in this latter scenario usually manifests as the assumption “I...

  12. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...tags due to the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular... Originally reported as a monomer16, IFP2.0 was later found to be a dimer3,15. ND-not determined in parallel...

  14. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...replaces a target region with a newly synthesized, templated sequence. And the story still isn’t over! Keep...Base Editor A base editor that relies on DNA glycosylation to create an abasic site. In this post, it refers...

  15. New Antibody Animation!

    Type
    Blog Post
    ...Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what ...

  16. New Coomassie Protocol Video

    Type
    Blog Post
    ...determine your antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant...

  18. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...101 covers everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting...

  20. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...whenever a new video is posted! If TikTok isn't your platform, all of our videos will also be shared on our ...
Showing: 781 - 800 of 974 results