We narrowed to 807 results for: MAK;

1. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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   ...relies on its activity on the target regions thus making it an affinity-based method. Due to the mismatch...

2. Evolution of Lab Techniques

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   ...popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic mouse lines...

3. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   ...model than in latent HIV-1 infection, which would make it easier for an “escape” virus to propagate and...

4. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   ...position. The “sloppy” PCR reaction is created by making some or all of the following modifications: 1) ...

5. Plasmids 101: Biotinylation

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   ...exposed side chain that can be easily manipulated to make reactive species without affecting the avidin binding...

6. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   ...allowing for stable expression of the transgene. This makes IDLVs an attractive choice for certain types of ...

7. Antibodies 101: Normalization and Loading Controls for Western Blots

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   ...controls So how can you address these errors without making incorrect assumptions? The answer depends on the...

8. Antibodies 101: Beyond Surface Labeling

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   ...gating uses the same color for different markers, making it easy to gate on the negative cell population...

9. CRISPR 101: Homology Directed Repair

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   .... Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et...

10. Hot Biosensors 2022: Year-End Roundup

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    ...iGECI and NIR-GECO2G, and its small size (~1500 bp) makes iGECInano suitable for AAV packaging.    Find iGECInano...

11. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ...register at a time. If a particular application makes it difficult to isolate single registers or if it...

12. Plasmids 101: Restriction Cloning

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    ...overhangs that allow them to bind. Ligase is used to make bonds between the insert and backbone covalent.  ...

13. Finding Your Perfect Job After University

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    ...Addgene’s Website and travel to meet scientists, making my job different every day. I love the freedom ...

14. A Control for All Seasons

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    ... high degree of variability in the process could make it difficult to obtain meaningful results.  Biological...

15. Plasmids 101: Repressible Promoters

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    ...adds a repressible element to this binary system (making this a repressible binary system). The GAL80 repressor...

16. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ...studies, the high gene copy number found in plants makes traditional knockouts unattractive from a practical...

17. Sequencing Options for CRISPR Genotyping

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    ... and do not require high sequencing read counts making them fast and viable options for multiplex sequencing...

18. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    ...selective expression of opsin genes in targeted cells makes it possible to control neuronal activity with a ...

19. Antibodies 101: Antibody Engineering and Directed Evolution

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    ...-antibody interactions in a plate-based format, making it suitable for comparing relative affinities across...

20. Deep Dive: Statistical Tests (Comparisons)

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    ... friendly neighborhood biostatistician happy by making your statistical analysis plan when you design ...