We narrowed to 952 results for: OMP;
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TypeBlog Post...in mice is possible (Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the...Cre not only halted the neurological damage but completely reversed it, regardless of age or symptom severity...
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Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...these people at the time, but I met some very accomplished scientists and some rising stars in the world...timetable for the Singletons in their bins. The next component of Binning Singletons is when we bring all the... -
Plasmids 101: Codon usage bias
TypeBlog Post...varied codon preferences among organisms aren’t completely understood, but some possible reasons include...codon optimization algorithms and DNA synthesis companies such as IDT and GenScript host online codon optimization... -
CRISPR 101: Any Base Transversion Editors
TypeBlog Post... Note: hypoxanthine (shown) is the nucleobase component of the nucleoside inosine. Created with BioRender.com...relied on deaminase enzymes, either to directly accomplish the base edit or to create the substrate for ... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...roughly measures granularity, a proxy for nucleic complexity of the cell. The sample will have been labeled...labeled using a panel of markers, which is comprised of antibodies conjugated to fluorescent markers and/... -
Viral Production at Addgene
TypeBlog Post...Purity Purity of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ... -
How to Write a Scientific Review Article
TypeBlog Post...Good luck! References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore. "Histone acetyltransferases...Addgene.org Check out Our Hot Plasmids Articles to See Compiled Info on New Plasmids Review the CRISPR Field with... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...is a terrible disease that is, unfortunately, completely preventable. Mainly caused by exposure to asbestos...non-resectable mesothelioma by the National Comprehensive Cancer Network, a coalition of the top 27 cancer... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...persistence of residual viral material [6]. The complete removal of the residual viral particles and establishment... in the presence of these two factors are not completely understood, the addition of an shRNA for P53 ... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...institutions doing? Institutions are now preparing to comply with the changes to the law on Dec 1st, according...centrally administered, which leads to many complications, including the inability of some institutions... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...ml before the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can alter expression...al. outline a do it yourself PCR-based approach complete with freely available positive controls. Routinely... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...Tarlow, Branden D., Milton J. Finegold, and Markus Grompe. "Clonal tracing of Sox9+ liver progenitors in ... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post...enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from Aggarwal ...size and reduced sensitivity to pH and calcium compared to previous lactate biosensors, and its affinity... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...HDR), a distinct cellular DNA repair pathway. Accomplishing this usually involves simultaneously introducing...mutation. Here, the blocking-only templates will compete with the blocking/intended mutation templates resulting... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...membrane-impermeant and can only enter cells with compromised plasma membranes — i.e., dead cells — while live...which yields a much weaker signal on live cells compared to dead cells. As a result, the largely negative... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...undetectable in the blood. But the virus doesn’t completely disappear; it just hides in latently infected...viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...enhancer regions (Mendenhall et al., 2013). By comparing gene activation when enhancers were active or ...increased gene activation at the targeted site compared to other Tet1 delivery systems. Transcriptional... -
Hot Plasmids: Spring 2025
TypeBlog Post... domain structure. Glypicans are proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG...). Figure 5: TurboCas protein labeling compared to immunoprecipitation. The top panel depicts ... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post... cell types. Figure 1: Diagram of AAV components. Created with BioRender.com. AAV vectors ...Transduction of Airway Epithelial Cells in Mouse Lungs Compared to That of AAV2 Vectors. Journal of Virology, ... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates which reduces...worms are perfectly viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted...