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We narrowed to 1,006 results for: RING

Showing: 781 - 800 of 1006 results

  2. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...tribulations of the process, I’ve provided guidance and mentoring to several people that found my insights helpful...'s about how YOU will fit into a department and bring in grants. What kind of colleague will you be? Do...

  3. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...donor, you can deliver a silent mutation in a neighboring nucleotide along with your desired edit. This...Concordet J-P. Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection...

  5. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...seems to be the case that there is no universal scoring system for selecting a gRNA, as the method of producing... is taken in the interpretation of results by requiring multiple sequences targeting a gene to score in...

  6. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...transferable skills while working in the lab and by offering advice on areas where you might need some help...Scientists: Managing v Leading Gain Management Skills Volunteering at a Professional Organization Resources on...

  8. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...looking for, but there are also benefits gained by lowering the complexity of the screen. For example, the..., Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach....

  9. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ... most common method is transfection of short interfering RNA (siRNA). Knockdown caused by siRNA is also...cytometry experiment. But validation is critical to ensuring accurate interpretation of your results. Therefore...

  10. CRISPR Guide

    Type
    Guide
    ...scientists have modified for a wide range of genome engineering techniques . Collectively, these techniques are...R epeat) technologies. Before CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) ...CRISPR rapidly became the most popular genome engineering approach. Figure 1: Overview of the basic CRISPR...first and come back to HDR . NHEJ is a naturally occurring repair pathway and is the primary repair mechanism... for DSBs. While this pathway is efficient in repairing DSB, it frequently causes small nucleotide insertions...: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR experiments involve editing more ...more than one gene. In these cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs...

  11. 2022 Depositor Week

    Type
    Blog Post
    ...revamped Data Hub! The community needs more data-sharing heroes like you.)  ...

  15. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...CRISPR campaign! But if you missed our tweets featuring the best of CRISPR in 2021 and useful resources...

  17. We’re Going Viral This Week!

    Type
    Blog Post
    ... post. Alongside our efforts to facilitate the sharing of reagents and data, we’re also publishing research...

  20. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...vial filling, accurate tube-labeling, quick plate pouring, and much more. We didn’t want to keep the knowledge...
Showing: 781 - 800 of 1006 results