We narrowed to 1,010 results for: RING;
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TypeBlog Post...donor, you can deliver a silent mutation in a neighboring nucleotide along with your desired edit. This...Concordet J-P. Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection...
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Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post...vivo probes, due to low autofluorescence, low scattering, and deep penetration of red-shifted light. The...about cellular events via fluorescence changes occurring due to protein synthesis, degradation, or reconstitution... -
March for Science
TypeBlog Post...Diversity and inclusion in science, technology, engineering and mathematics (STEM) (17). This effort is two-fold... “politicized science” ABC News: White House considering gutting 38 EPA programs: draft budget Science... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog Post...stain-free imaging systems are both effective for measuring the total protein present in a lane (Aldridge ...individual protein. This ensures you are truly comparing the abundance of your POI as a fraction of the... -
Tips for Getting a Faculty Position
TypeBlog Post...tribulations of the process, I’ve provided guidance and mentoring to several people that found my insights helpful...'s about how YOU will fit into a department and bring in grants. What kind of colleague will you be? Do... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog Post...sequence detection method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127... Detection of SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...editing tailored to smaller patient populations. To bring DMD CRISPR therapy closer to the clinic, much work... Mary Gearing... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog Post...seems to be the case that there is no universal scoring system for selecting a gRNA, as the method of producing... is taken in the interpretation of results by requiring multiple sequences targeting a gene to score in... -
Transferable Skills Guide: Teamwork
TypeBlog Post...transferable skills while working in the lab and by offering advice on areas where you might need some help...Scientists: Managing v Leading Gain Management Skills Volunteering at a Professional Organization Resources on... -
Important Considerations When Using AAVs
TypeBlog Post...tracing techniques: exploiting the offspring of genetic engineering." Journal of neuroscience methods ... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...looking for, but there are also benefits gained by lowering the complexity of the screen. For example, the..., Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach.... -
Antibody Validation for Flow Cytometry
TypeBlog Post... most common method is transfection of short interfering RNA (siRNA). Knockdown caused by siRNA is also...cytometry experiment. But validation is critical to ensuring accurate interpretation of your results. Therefore... -
2022 Depositor Week
TypeBlog Post...revamped Data Hub! The community needs more data-sharing heroes like you.) ... -
Help Us Name Blugene’s New Virus Friend
TypeBlog Post...finding a good name for Blugene’s virus friend. During the month of January, we’ll be taking name suggestions... -
Introducing the Addgene Antibodies 101 eBook!
TypeBlog Post...to be a helpful desktop reference. Each chapter brings together all of our 101 blog posts on an antibody-specific... -
Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More
TypeBlog Post...the nervous system, and vectors for zebrafish engineering. You can find additional hot plasmids in our ... -
The 12 Days of CRISPR: 2021
TypeBlog Post...CRISPR campaign! But if you missed our tweets featuring the best of CRISPR in 2021 and useful resources... -
We’re Going Viral This Week!
TypeBlog Post... post. Alongside our efforts to facilitate the sharing of reagents and data, we’re also publishing research... -
#Deckthelab Contest Winners 2017
TypeBlog Post... Although we're storing away all of our holiday decorations, our holiday spirit remains. We would like... -
Participate in the Pets of Addgene Scavenger Hunt
TypeBlog Post...navigate the scavenger hunt. Good luck and have fun exploring the blog! ...