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Addgene
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  2. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...self-supporting service so that Addgene is not dependent on grants. Aiming to operate under a similar model for our...scientists and the support of their technology transfer offices, Addgene would not be able to have such...

  3. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...native endonuclease activity degrades the displaced strand.   Figure 1: EvolvR consists of an error-prone...lineage tracing cells that do not tolerate double-strand DNA breaks by introducing a unique tag into a genomic...

  6. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...next few years, with the goal of creating more transparent and reproducible science in the long run (Hohlbein...2020) Hongquan Li, Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew Chai, Manu Prakash, ...

  7. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ... a function in a given process. The process can range from a defined pathway or reaction to a complex ...phenotypical outcome. In forward screens, there is random or unknown manipulation of the population. This...

  8. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...categorization of our information in order to more granularly customize the search engine, and a scoping mechanism...example, matches of a name in the depositor field will rank higher than matches of the same name in the publication...

  10. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...fluorescent proteins in the same spectral class (Cranfill et al, 2016). This non-linear dependence on excitation...the conclusions reached at high power may not translate to real applications in which far less power is...

  11. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...spatial relationship of the two genes over time, at a range of distances from 2-75 Mbp. Labeling multiple, intrachromosomal...a system has been previously described for transcriptional activation/repression and genome editing. ...

  14. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...bonds, monobodies can be easily expressed in transfected eukaryotic cells, allowing for them to be used...an excellent option for many applications, and frankly, if ain't broke, don't fix it!  That being said...

  15. Antibodies 101: Selecting the Right Antibody

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    Blog Post
    ...-independent assays such as mRNA data from transcriptomics; independent antibody strategies that compare...validation does not mean that the antibody is guaranteed to work. Antibody-based assays are complex and...

  16. Developing Lab Management Software for Biology

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    Blog Post
    ... lab, a similar situation could arise if a lab veteran who worked with a particular sample suddenly left...t have a system through which she could easily transfer her instructions for dealing with the samples ...

  18. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...sure the feature you want to verify is within this range. Read some of our other blog posts for more tips...production and storage, we grow a little extra and transfer 100 µl of glycerol stock to each of 2 96-well ...

  19. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...library was developed when Carrie was a post-doc in Frank Stegmeier’s lab in Novartis Oncology.  Bhang did...lentiviral vectors with roughly 73 million semi-random, 30 bp DNA barcodes that are integrated into target...
Showing: 781 - 800 of 988 results