We narrowed to 934 results for: TIM;

1. Viral Vectors 101: Transductions

   Type

   Blog Post

   ...design and construction steps are complete, it’s time to make virus! The constructed viral vector containing...

2. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

   Type

   Blog Post

   ...manuscript. As a non-native English speaker, I sometimes miss the sensitivity to judge how aggressive my...

3. New and Upcoming Viral Vectors - Spring 2019

   Type

   Blog Post

   ...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

4. Antibodies 101: Epitope Availability

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   Blog Post

   ...antibodies, which only recognize one epitope. (It can sometimes be an issue with polyclonal antibodies, so it'...

5. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

   Type

   Blog Post

   ...eliminates the double-stranding step and reduces both the time and cost for AAV genome sequencing.  Figure...

6. Celebrate Open Data Day with Addgene’s AAV Data Hub!

   Type

   Blog Post

   ...titer dose (Example: 1E13 GC/mL) and subject age at time of injection. How long post-injection did you wait...

7. New and Upcoming Viral Vectors - December 2019

   Type

   Blog Post

   ...localize opsins to the soma to avoid off target photostimulation of axons and dendrites on neighboring neurons...

8. Hot Plasmids and Viral Preps - September 2021

   Type

   Blog Post

   ...variety of natural products. Recently, this group optimized their initial system by using a stronger promoter...

9. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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   Blog Post

   ... B.1.617.2 (Delta variant) was not tested at the time of this study. Figure 1: Neutralization ...

10. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type

    Blog Post

    ...I had to cut it very abruptly to stay within the time limit. pic.twitter.com/j6rhCCby2m — Barbara Di Ventura...

11. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type

    Blog Post

    ...reagents and will enable researchers everywhere.” The timing is ideal as the NIH’s BRAIN Initiative has been...

12. Fluorescent Biosensors for Measuring Autophagic Flux

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    Blog Post

    ...acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio: a decrease in GFP fluorescence...

13. 10 Great Guest Posts We're Thankful For

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    Blog Post

    ...from the post and the associated publication. Optimizing Donor DNA for Enhanced CRISPR Genome Editing ...

14. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    Blog Post

    ...requires less than eight hours worth of hands-on time. Creating a modular, selection-based gene tagging...

15. Another Pathway into Cells: iTOP

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    Blog Post

    ...Compared to CCP transduction, iTOP is at least four times more efficient in primary fibroblasts. iTOP also...

16. Creating accessible biology activities in schools with BioBits

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    Blog Post

    ...teaching developmental biology Use the pathways over time plasmids to engage students in functional genomics...

17. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    ...these tools. Years ago, Kurt Thorn (UCSF at the time) began an effort to collate some of the current ...

18. The Importance of High Titer for AAV Transductions

    Type

    Blog Post

    ...Nonetheless, there are a variety of ways you can optimize AAV production. We’ve listed some simple steps...

19. New and Upcoming Viral Vectors - June 2019

    Type

    Blog Post

    ...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

20. Improving Transduction Efficiency with Exosome AAVs

    Type

    Blog Post

    ...PubMed Central PMCID: PMC5728288. Schiller, Lara Timantra, et al. "Enhanced Production of Exosome-Associated...