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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 841 results for: ang

Showing: 781 - 800 of 841 results

  3. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...enzyme, or enzymes that have compatible overhangs or no overhangs after digestion, you will need to use ...chance for mis-priming, so you can use a pretty wide range of annealing temperatures, but you may need to increase...cloning. DNA replication by PCR has error rates that range from roughly 1 per 500bp to roughly 1 per 10 million...

  4. AAV ddPCR Titration

    Type
    Protocol
    ...outlined in this protocol are based on an AAV titer range of 5E+12–5E+13 GC/mL, where GC is genome copies ...slowly to avoid generating aerosols. Be sure to change tips each time. Dilution 1 (20X): 5 µL in 95 µL...directly in the center of the well and tilt to a 45° angle. Count to 20 while slowly and gently aspirating ... of the well and tilt the pipette tips at a 45° angle. Count to 20 while slowly and gently dispensing ...

  12. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ... a short stretch of DNA to a plasmid, such as: Changing a Multiple Cloning Site (MCS) Adding short tags...once annealed can be cloned directly into the overhangs generated by restriction digest of existing sites...to include additional bases to complement the overhangs generated when digesting the vector with EcoRI...

  13. Centrifugation

    Type
    Protocol
    ... tubes. Depending on the centrifuge their speed ranges can vary, but are typically lower than some other...unbalanced state can damage the centrifuge and be dangerous for the user. If you do not have a balanced number...are not the same units and are not directly interchangeable. If you need to convert between the two, use...

  14. Gibson Assembly Protocol

    Type
    Protocol
    ...two-part Gibson reaction if you're only making a small change in a plasmid (such as point mutations). Generate...T5 Exonuclease - creates single-strand DNA 3’ overhangs by chewing back from the DNA 5’ end. Complementary...England Biolabs by TelesisBio. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO. (2009). ...

  17. Antibodies 101: Validation

    Type
    Blog Post
    ...non-specific, background signal, so you’ll need a range of expression strengths to be confident in your ...

  19. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ... a single stranded overhang on the digested end of the DNA fragment. The overhangs, called "sticky ends...sticky ends are compatible, meaning that the overhanging base pairs on the vector and insert are complementary...
Showing: 781 - 800 of 841 results