... to wane about a month later, the animal is given additional booster immunizations every 2-3 weeks to ...  Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...

...and best characterized of these is the Src homology 3 (SH3) domain family, known to be involved in cytoskeletal... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...can take as long as several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used...

...PMID: 21364278. PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene ...Resources on the Addgene Blog Learn More About Lentivirus Learn more About AAV Get the Scoop on Our Viral Service...

... to antibody response 2) very high immunogenicity 3) and long-lasting immune response even after a single...Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one for ...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

...synthetic biology tools for cyanobacteria. Figure 3: Assembly of plasmids using the Cyanogate Kit and ... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...tracing using the transgenic zebrafish model, ubi:zebrabow. In this system, cells express various fluorescent...

...10) and grow them in a small culture volume (2 or 3 ml) containing the corresponding antibiotic, extract...they get old, they lose competency). If all the above is correct, you should probably have a second and...blog posts Read blog posts on plasmid cloning Read about different plasmid elements Resources on Addgene.org...

...ssRNA engaging one strand of a dsDNA molecule; the 3-stranded structure produced is known as an R-loop....power of dsRNA for a long time, even before we knew about all the diverse in vitro production mechanisms. ...repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular processes...

...PubMed PMID: 2839833. PubMed Central PMCID: PMC281709. 3. Tissue– and site–specific recombination in transgenic...discussed below. How does it work? As alluded to above, the Cre recombinase catalyzes the site specific...Combining this with some of the loxP methods described above, a genetic modification can be restricted based ...

...spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s found in other species can recognize...our CRISPR 101 blog posts Find more information about Cas proteins Download the CRISPR 101 eBook Resources...

...transfection. The other (mKate2) is regulated in the 3’ UTR by four miRNA target sites and thereby serves... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...