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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 993 results for: Ran

Showing: 801 - 820 of 993 results

  1. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...sure the feature you want to verify is within this range. Read some of our other blog posts for more tips...production and storage, we grow a little extra and transfer 100 µl of glycerol stock to each of 2 96-well ...

  2. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...library was developed when Carrie was a post-doc in Frank Stegmeier’s lab in Novartis Oncology.  Bhang did...lentiviral vectors with roughly 73 million semi-random, 30 bp DNA barcodes that are integrated into target...

  5. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...research or larger journals that publish about a broad range of topics.  Twitter paper bots: Twitter bots tweet...you expand your search. Results in your feed are ranked based on their influence on the network, or body...

  6. CRISPR in the Clinic

    Type
    Blog Post
    ...CRISPR targets — Sickle Cell Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which...clinical trial for SCD in 2022 as well. Hereditary transthyretin amyloidosis (hATTR) hATTR is caused by a variety...

  7. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...approved drugs so that your results are viable for translation to human patients. Picking receptors The receptor... specifically designed Gi-coupled receptor in transgenic mice. Nature Biotechnology 17:165–169 . https...

  8. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    .... The activity of SpRY was validated in double stranded cleavage and base editing applications, with high...solution? Almost! Assuming a PAM of NRN, 50% of randomly selected genomic loci will have a PAM located ...

  9. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...fast-tracked development of diagnostics. Mostly transmitted by Aedes mosquitoes (aegypti and albopictus) ...and electronic companion technologies that has transformed the assay’s capabilities. Specifically this includes...

  10. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...tissue, placement above the site of stimulation guarantees that the majority of the opsins will receive ...tangled. Furthermore, to give the animal the full range of motion, a commutator allows the patch cable to...

  12. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...month - with only occasional supplementation with grant funds, money is always tight. Of course, unlike ...becomes obsolete amazingly fast, but with a wide range of skills among our members we’ve managed to learn...

  13. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...of an exogenous small molecule). Degron tags can range in size from several kDas up to ~35 kDa and are ... because the system modulates protein post-translationally, unlike many other methods. Secondly, these...

  18. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...olds in a single evening. Grades K-5 cover a broad range of attention spans, skill levels, and STEM exposure...aspects of talking to 8th graders was the broad range of questions I received. I gave my presentation ...

  19. Plasmids 101: Protein tags

    Type
    Blog Post
    ...usually smallish peptides incorporated into a translated protein. As depicted in the accompanying cartoon...peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant proteins...

  20. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...where each base is recognized and recorded as a DNA strand is synthesized (Figure 1). Sanger sequencing requires...“nanopores” that are attached to the flow cell membrane and have an electrical current running through...
Showing: 801 - 820 of 993 results