Skip to main content
Addgene
Showing: 801 - 820 of 1011 results

  1. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations...understand isoform-specific gene regulation — at least, more straightforward than generating cDNA for each...

  2. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...easy to export data from the ELN directly into at least one and preferably more than one of these five general...other side of the pond through interacting with colleagues in Edinburgh. You can follow Rory on Twitter ...

  3. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...base in genomic DNA without double-stranded DNA cleavage." Nature 533.7603 (2016): 420. PubMed PMID: 27096365...editing of A• T to G• C in genomic DNA without DNA cleavage." Nature 551.7681 (2017): 464. PubMed PMID: 29160308...

  5. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ... viral vector preparation protocol, and we are pleased to report that over 95% of viral capsids in representative...Resources on Addgene.org Find All Available AAV Learn More about Virus Production Find Viral Vector Protocols...

  6. Hot Plasmids - November 2023

    Type
    Blog Post
    ...ticker tape By Brian O’Neill The Cohen lab and colleagues recently created a system for recording the transcriptional...Application section of the Addgene antibody page, and learn more about the whole-brain reference atlas efforts...

  7. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...only 20 amino acids and 3 stop codons to encode leaving 41 codons unaccounted for. The result is redundancy...organism. So what are the evolutionary constraints that lead to these preferences and what can we do about them...

  8. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...optimization within your experiment. Read on to learn more about the various controls that can be used...biological controls is independence: at the very least you should repeat your whole experiment from start...

  9. March for Science

    Type
    Blog Post
    ...governments exist in reality and science is how we learn about reality. Formally, a government can be defined...public discourse, many scientists have chosen to leave the lab and march. Unintended consequences While...

  10. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...loop-structure (Querido and Chartrand, 2008). Finally, a nuclease-deficient Cas9 can bind loci in the genome that...interfere with the function of the protein. This is clearly the case when proteins undergo post-translational...

  11. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...tip: When designing your PCR, ensure there is at least ~200 base pairs of sequence flanking the editing...find a null clone, you should screen, at the very least, 4 clones. Did you use a dual guide system to generate...

  12. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... in the classroom on our blog. Now that you’ve learned more about popular model organisms in the lab, ... about plasmids in our Plasmids 101 blog series Learn more about CRISPR and genome editing in our CRISPR...

  14. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... 1). In general, the monomeric state of a probe leads to less interference with the oligomeric state of...indicators NIR-GECOs were developed based on mIFP6. Learn more about the characteristics, performance, and...

  15. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...multiplexing gRNAs here). Cas9 is commonly tagged with a nuclear localization sequence to enhance targeting to the...plant species or cell type (Belhaj et al., 2013). Nuclease dead Cas9 (dCas9) based activators (such as dCas9...

  16. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...(7). This modification allowed the deletion of release factor 1, the protein responsible for terminating...reference and protocols for use of this system, please reference ‘Rinehart Lab reagents for improved expression...

  17. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...fungus Neurospora crassa. The QUAS promoter is less leaky than UAS in the basal state, and co-expression of...expression. Co-expression of repressor QS along with QF leads to intermediate expression from QUAS, as seen with...
Showing: 801 - 820 of 1011 results