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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,028 results for: LEA

Showing: 801 - 820 of 1028 results

  1. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...AAV5. AAV9 preps and more AAV1 versions will be released soon. Visualize axonal projections and putative...

  4. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...temperature). Photostability: Fluorescent molecules gets bleached (i.e. lose the ability to emit light) after prolonged...imaging technologies to study gene expression, nuclear localization, and dynamics” Curr. Opin. Cell. Biol...

  7. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...target the CNS or the PNS, respectively.  Read on to learn more about these two new AAV capsids and how they... Blog Important considerations when using AAVs Learn more about using AAV as a tool for gene expression...

  9. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...After uncoating, reverse transcription follows the release of the two strands of viral RNA and of the viral...genome can direct the production, packaging, and release of progeny viral particles. And there you have ...

  10. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...called a standard. The ideal standard curve has at least five dilutions, with each step of the series diluted...good” is my standard curve? A poor standard curve leads to over or underestimation of an unknown sample’...

  11. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene expression in...development. As a result, Luk Vandenberghe and colleagues at MEEI co-founded a non-profit to help push ...

  12. MXS Chaining

    Type
    Blog Post
    ...TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag (derived from the tyrosine-protein...on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids ...

  13. CRISPR Antimicrobials

    Type
    Blog Post
    ...antimicrobials using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed... Bikard, David, et al. “Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials.” ...

  14. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...converts the raw sequencing data to FASTQ format, cleans and normalizes reads using the BBTools suite, and...This process saves the QC team time, but does not leave much flexibility for discrepancies in backbone sequences...

  15. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...embryogenesis (Figure 3). mScarlet-I3 also offered clearer signals due to reduced background signal when tested... gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain carrying Cas9 to allow the introduction...

  16. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations...understand isoform-specific gene regulation — at least, more straightforward than generating cDNA for each...

  17. Hot Plasmids - November 2023

    Type
    Blog Post
    ...ticker tape By Brian O’Neill The Cohen lab and colleagues recently created a system for recording the transcriptional...Application section of the Addgene antibody page, and learn more about the whole-brain reference atlas efforts...

  19. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...easy to export data from the ELN directly into at least one and preferably more than one of these five general...other side of the pond through interacting with colleagues in Edinburgh. You can follow Rory on Twitter ...

  20. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...base in genomic DNA without double-stranded DNA cleavage." Nature 533.7603 (2016): 420. PubMed PMID: 27096365...editing of A• T to G• C in genomic DNA without DNA cleavage." Nature 551.7681 (2017): 464. PubMed PMID: 29160308...
Showing: 801 - 820 of 1028 results