We narrowed to 910 results for: Nes

1. The Importance of High Titer for AAV Transductions

   Type

   Blog Post

   ...lot of AAV particles are needed to get a few good ones into a cell to produce expression. The higher the...

2. Hot Plasmids - January 2023

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   Blog Post

   ...-head for gene expression changes of non-target genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic...

3. Celebrate National Book Month with Addgene

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   Blog Post

   ...Brain by Oliver Sacks "Neurologist Oliver Sacks examines the relationship between the human brain and music...

4. New and Upcoming Viral Vectors - September 2019

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   Blog Post

   ...variant has an approximately 12-fold increase in brightness after ~100 action potentials. This is about twice...

5. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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   Blog Post

   ...modeling, directed evolution, site-saturation mutagenesis, and medium-throughput screening, she increased...

6. Improving Transduction Efficiency with Exosome AAVs

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   Blog Post

   ...cells in the inner ear in a mouse model of human deafness (György et al., 2017), exo-AAV2 in mouse retina...

7. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

   Type

   Blog Post

   ...by glutamate, and tweaking it to improve its responsiveness, they showed that iGluSnFR could detect neuronal...

8. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

   Type

   Blog Post

   ...read and follow the journal’s specific author guidelines for reporting your materials. If you deposit ...

9. Four Factors that Differentiate the Stem Cell Field

   Type

   Blog Post

   ...as informed consent (How do we prevent stem cell lines from becoming the new HeLa cells?), genome engineering...

10. How to Lead a Great Meeting

    Type

    Blog Post

    ...heard. Here's a template for team meetings. One-on-Ones: Managers should set up regular check-ins with their...

11. Addgene Welcomes John Doench to the Advisory Board

    Type

    Blog Post

    ... be done with RNAi in terms of determining what genes are involved in biological process X, you can do...

12. Finding and Joining Your Dream Lab

    Type

    Blog Post

    ...those who notice and embrace the “odd and extreme openness” of the lab (i.e projects like the Personal Genome...

13. Improved Plasmid Maps Powered by SnapGene

    Type

    Blog Post

    ... plasmid features such as antibiotic resistance genes, selectable markers, promoters, origins, tags, and...

14. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post

    ...Lastly, an AAV vector was used to multiplex edit 3-4 genes in a primary culture of mouse neurons and in vivo...

15. Communicating Your Science With Help From ComSciCon

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    Blog Post

    ...for its immense importance, it may well be what determines whether you get the job after your next interview...

16. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post

    ...for bioinformatic data mining. As we continue to harness the natural diversity of CRISPR systems, only time...

17. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    ...arrangement of the B-B’ and C-C’ palindromic sequences determines the orientation of the ITR, which can either ...

18. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post

    ...function. By using AAVrg in Cre transgenic mouse lines, you can access and manipulate an even further defined...

19. 7 Tips to Secure a STEAM Internship This Summer

    Type

    Blog Post

    ...ahead for success. Every student should use these guidelines consistently across a number of years as you ...

20. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    ... alternative initiation, so they mutated the methionines at position 14 and 21 to create GEmCherry2. The...