We narrowed to 1,052 results for: STI

...Structural details All this, and scientists are still working out important details in their understanding... tool. A week later, researchers at the Broad Institute and MIT published another report showing Cas9'...

...- increasing open source sharing and helping us stick to our core mission. Chapter 13: Outro - remember...stem cell research as an NIH-funded Principle Investigator in the Kotton Laboratory and is the founding...

...new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR-based marker excision method for marking distinct cell clones developed by the Michalis Averof lab...

...specificity engineering. “The RNA binding domain is interesting because by changing certain amino acids you can...lose their solubility. Her lab is currently investigating the issue and hopes to have a better understanding...

...project involving multiple scientists, create and stick to timelines Be a mentor in a formal program Start...Collaboration and teamwork One of the defining characteristics of nonacademic science careers is that a team...

...individual plasmids to 5,000 different research institutions. This has given us a unique window into technology...plasmid distributed in our 1st year, 2004, and it still gets requested! Fire Lab C. Elegans Kit - Our first...

...anchors for genome engineering applications was still very new. Now, Konermann and her colleagues including...transcription in as little as 30 minutes after light stimulation. Under those optogenetic controls, gene expression...

...quality control. Through our partnership with the Institute for Protein Innovation (IPI), we expanded a growing...repository can now be packaged on demand in five distinct serotypes, offering you unparalleled flexibility...

...new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...of ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA...

...our viral service in late 2016 and requests are still pouring in! While our lentiviral service is going... many more tips and ideas on how to use these fantastic tools in your experiments.  Many thanks to Hongkui...

...the Genetic Perturbation Platform at the Broad Institute, John Doench. A genetic screening project can ...at a more-typical scale of tissue culture while still offering the potential for true biological insight...

...CHimeric IMmune Editing system) to enable the constitutive, ubiquitous knockout of single genes in immune...To demonstrate the power of these systems to investigate in vivo mechanisms, we targeted two phosphatases...

... in cancer Previously available methods for investigating and functionally validating the thousands of...useful for? They could be used to more thoroughly investigate any of the dozens of wild-type genes and their...

... FPs since its emission wavelength (612 nm) is distinct from other commonly used FPs, and GFP does not...localization that have been encountered by some investigators when using forms of mEos." The ability to repurpose...

..., designed for use in B. subtilis, contains the MISTIC (membrane-integrating sequence for translation ...integral-membrane protein constructs) protein tag. MISTIC drags tagged proteins into the membrane and may...

... inferred based on correlative biochemical characteristics and ectopic reporter assays. We have been interested...Bauer is a pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical...

...? This final post in the PI Interview Series investigates how these three PIs reward their team and manage...during the child-rearing years, learning “lots of interesting things from children, their classmates, and parents...

...frequently used pages in your notebooks and go wild with sticky notes and labels. Color code your Post-its and ...write brief descriptions on each. Place the notes sticking up out of the top of the notebooks, so that you...

...localization of individual proteins, but also investigate protein-protein interactions via FRET. Sidae ...partial gene deletion, overexpression (by promoter substitution), and tagging at either the N- or C-terminus...

...sought to support scientists at all research institutions. In 2012, we shipped our first plasmid to an... Laboratory. Through our partnership with the Institute for Protein Innovation, Addgene also distributes...