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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,035 results for: URE

Showing: 801 - 820 of 1035 results

  1. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...vision in a canine model of childhood blindness. Nature Genetics 28, 92-95. PubMed PMID: 11326284. 4. Bennett...L., 2015. Gene Therapy returns to centre stage. Nature Vol 526, p.351. PubMed PMID: 26469046. 6. The Forever... Rev Virol. 2014 Nov;1(1):427-51. DOI: 10.1146/annurev-virology-031413-085355. PubMed PMID: 26958729.  ...

  2. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...and Fultineer analyzed the gnomAD dataset, which captures a broader swath of human genetic diversity, to...Project: a global resource to map genomic diversity. Nature 604, 437–446 (2022). https://doi.org/10.1038/s41586...weaponization of genetics research by extremists. Nature 610, 444-447 (2022). doi: 10.1038/d41586-022-03252...

  3. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... mean a world of mental relaxation. And, I’m not sure I should put this in print, but I actually let my... my daughter skip school a couple of times. Be sure to add time with your family, for yourself and for...just “do science” – we are scientists. We have to figure out a way to keep being ourselves and to be happy...

  4. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...behavioral tasks was an elevated plus maze that measures risk aversion/anxiety. The mouse can move freely..., learning, and memory. We’ll cover these in a  future blog post, so stay tuned!  References Alpár, ...rapid and long-lasting antidepressant effects." Nature communications 10.1 (2019): 223. PubMed PMID: 30644390...

  7. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...and S. cerevisiae (Tuller et. al., 2010). Figure 1: An overview of peptide synthesis. A ribosome...as Hoogsten base pairs and wobble pairs.  Figure 2: (A) The codon wheel depicts the 64 codons that...PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon usage bias, and...

  8. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...The detectable signal from this reporter can be measured and quantified to determine the presence and quantity...lateral flow strip format. They published this in Nature Biotechnology. Like the SHERLOCK detection system...2020) CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020...

  9. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ..., the envelope gene, and a polyA tail.   Figure 1: Lentiviral production uses three plasmids: (...in Medical Virology (Li et a., 2018).   Figure 2: To pseudotype with the SARS-CoV-2 spike protein..., Bieniasz PD, Jäger U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti...

  10. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...Addgene. In vitro screening for the best gRNA Figure 1: Validation of double strand break (DSB) mediated...were fusing, you may have several mosaic mice. To figure out what alleles are present in these mice, you...favourite mammalian model organisms in the near future.  Many thanks to our guest blogger Samantha Young...

  11. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...than the other (Sandomenico et al., 2020). Figure 1: Comparison between the IgG antibody and scFv...of panning and scFv characterization.    Figure 2: Phage display starts with a pooled library of...Bates A, Power CA (2019) David vs. Goliath: The Structure, Function, and Clinical Prospects of Antibody ...

  12. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... dsDNA breaks: following a cut, the error-prone nature of non-homologous end joining (NHEJ) often leads...database, which relies on CAGE-seq to directly capture the mRNA cap, provides the most accurate mapping...examined the ability to use sequence-based and other features to nominate gRNAs that are likely to be active...

  14. CRISPR Guide Update

    Type
    Blog Post
    ...accompanied by new or refreshed figures made using BioRender.com. Figure 1: PRIME editing Please...

  15. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...the first day of CRISPR, Addgene gave to me: a miniature CRISPR-Cas system for mammalian use. On the second...Addgene gave to me: programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR...

  16. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    ... significantly away from the functionality and features that previously made the platform a mainstay of...not delete @addgene on X. This will additionally ensure that the @addgene handle is always associated with...

  17. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...interrogate many scientific questions. But these tiny creatures do tend to come with a rather astonishing ability...system for locating and containing escaped flies. Nature 4:1-22. https://doi.org/4.01.2022/hafd...

  18. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    ...sharing all the incredible guest posts and stories featured in our blog in 2023. Some of these were written...Course-Based Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew Acoustic Reporter Genes...

  20. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ... by Xi Chen (@seleena78)   Honorable Mention: Culture Warrior The Moore lab, who was full of holiday ...honorable mention for their second submission.    Our Cultured Warrior (made by Nate using pipette boxes, wire...
Showing: 801 - 820 of 1035 results