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We narrowed to 1,090 results for: addgene

Showing: 801 - 820 of 1090 results
  1. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    Blog Post
    ...perform pha-1 co-conversion are available through Addgene. In 2019, Farboud et al. described another similar...To see more CRISPR tools for C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives...about genome editing with CRISPRs? Check-out Addgene's CRISPR Resources Learn about gRNA design and...
  2. Antibodies 101: Flow Cytometry

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    Blog Post
    ...fluorescent microscopy technique for your study see Addgene’s blog post Which Fluorescence Microscopy Techniques...experiment and are unsure which to choose, check out Addgene’s article: Which Fluorescent Protein Should I Use.../s10616-011-9415-0 Additional resources on the Addgene blog Check out other methods using fluorescent...proteins Learn more about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody...
  3. Selecting Your Plasmid Purification Kit

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    Blog Post
    ...reduce your lab’s environmental impact, check out Addgene’s blog post on bringing sustainable practices for...relatively little work after the initial setup. (Addgene actually uses some of these — if you’re curious...Nyla Naim. Resources Additional resources on the Addgene blog Plasmid Preps: Different Purity, Different...: Easy Action Items Additional resources on addgene.org DNA Quantification Protocol Recovering Plasmid...
  4. Antibodies 101: Designing Your First Flow Panel

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    Blog Post
    ...Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository Antibody...sailing, and reading.   More resources on the Addgene blog Antibodies 101: Introduction to Gating in ...
  5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    Blog Post
    ...advancement. Andrew Hempstead is a Senior Scientist at Addgene.  Andrew's interests include genome engineering...PMCID: PMC5681539. Additional resources on the Addgene Blog Subscribe to CRISPR blog posts Learn about...techniques Find tips on gRNA design Resources on Addgene.org Browse all CRISPR plasmids Find validated gRNAs...
  6. 10 Basic tips for mammalian cell culture

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    Blog Post
    ...many times and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage...Second  Edition), 2012. Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian...Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your research Find...
  7. Adenoviral Vector Production and Troubleshooting

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    Blog Post
    ...recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide webpage, and references 1, 2....PMCID: PMC5609467. Additional Resources on the Addgene Blog Using virus in your research - a primer for...troubleshooting viral transductions Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page...
  8. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post
    ...this paper are available at Addgene.  Additional Resources on the Addgene Blog Learn about Engineered...about Homology Directed Repair Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids...
  9. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    Blog Post
    ...constructs you create in your own work available to the Addgene community. Download our Markers of Subcellular ... FRET Microscopy. Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous ...Collection Get Tips for Using FRET Resources on Addgene.org Browse the Allen Institute for Cell Science ...
  10. Truncated gRNAs for Regulating Gene Expression

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    Blog Post
    ...    Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory at Columbia...PMCID: PMC4265475. Additional Resources on the Addgene Blog Listen to Our Interview with Alex Chavez ...: A Practical Guide Additional Resources on Addgene.org Check Out Our CRISPR Guide Pages Find Empty ...
  11. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post
    ...bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References Auchtung, Jennifer M., et al. "Biology...PubMed PMID: 26104363. Additional resources on the Addgene blog Learn about other CRISPR methods for bacterial...libraries Read all CRISPR blog posts Resources on Addgene.org Browse our CRISPR interference plasmids Find...
  12. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    Blog Post
    ...at existing tools. As has been written here on Addgene, there has been a Cambrian-like explosion of software... Clement Andy May Additional resources on the Addgene blog Read our CRISPR 101 blog posts Learn about...gRNAs Find other CRISPR software Resources on Addgene.org Find CRISPR plasmids and resources Download ...
  13. Summer SciComm Series: Modes of Communication

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    Blog Post
    ...                Two of the platforms we use at Addgene: YouTube, for longer educational videos, and Twitter...Internet "trolls"   More resources on the Addgene blog:  Summer SciComm Series: Cognitive LoadIntro...
  14. Polymerase Chain Reaction Overview and Applications

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    Blog Post
    ...concentration of the original template DNA in the sample.  Addgene currently uses ddPCR for AAV titrating. You can...Elsevier, pp 215–247 Additional resources on the Addgene blog Droplet Digital PCR for AAV Quantification...Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol...
  15. Of Myc and Men

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    Blog Post
    ...Antibody Resources on Addgene.org  Find recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the...scisignal.aax9730  Additional resources on the Addgene blog Antibodies 101: Monoclonal Antibodies Plasmids...
  16. Academic vs. Industry Postdocs

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    Blog Post
    ...choosing a lab Resources on Addgene.org Find open positions at Addgene Check out our career videos ...neurodegenerative diseases.   Additional resources on the Addgene blog Check out all careers related blog posts ...
  17. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post
    ...standardize your experiments later. Stay tuned to the Addgene blog and be sure to check out our viral vector ... PMCID: PMC330758. Additional Resources on the Addgene Blog Learn All about Adenovirus Associated Virus...Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages Find ...
  18. Plasmids 101: Colony PCR

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    Blog Post
    ...back-bone specific primers. Find Empty Backbones at Addgene Each approach has advantages and drawbacks, which...Overview of Colony PCR Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC ...about Gibson Assembly Additional Resources on Addgene.org Read about Choosing a Molecular Cloning Technique...
  19. Which Fluorescent Protein Should I Use?

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    Blog Post
    ...More examples of these biosensors can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be...More Helpful Websites & Resources: FP guide at Addgene Interactive Visualization of Fluorescent Protein...
Showing: 801 - 820 of 1090 results