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We narrowed to 1,091 results for: addgene

Showing: 801 - 820 of 1091 results

  2. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...                Two of the platforms we use at Addgene: YouTube, for longer educational videos, and Twitter...Internet "trolls"   More resources on the Addgene blog:  Summer SciComm Series: Cognitive LoadIntro...

  3. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...concentration of the original template DNA in the sample.  Addgene currently uses ddPCR for AAV titrating. You can...Elsevier, pp 215–247 Additional resources on the Addgene blog Droplet Digital PCR for AAV Quantification...Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol...

  4. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...advancement. Andrew Hempstead is a Senior Scientist at Addgene.  Andrew's interests include genome engineering...PMCID: PMC5681539. Additional resources on the Addgene Blog Subscribe to CRISPR blog posts Learn about...techniques Find tips on gRNA design Resources on Addgene.org Browse all CRISPR plasmids Find validated gRNAs...

  5. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...many times and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage...Second  Edition), 2012. Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian...Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your research Find...

  6. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide webpage, and references 1, 2....PMCID: PMC5609467. Additional Resources on the Addgene Blog Using virus in your research - a primer for...troubleshooting viral transductions Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page...

  7. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...this paper are available at Addgene.  Additional Resources on the Addgene Blog Learn about Engineered...about Homology Directed Repair Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids...

  8. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...constructs you create in your own work available to the Addgene community. Download our Markers of Subcellular ... FRET Microscopy. Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous ...Collection Get Tips for Using FRET Resources on Addgene.org Browse the Allen Institute for Cell Science ...

  9. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...    Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory at Columbia...PMCID: PMC4265475. Additional Resources on the Addgene Blog Listen to Our Interview with Alex Chavez ...: A Practical Guide Additional Resources on Addgene.org Check Out Our CRISPR Guide Pages Find Validated...

  10. Of Myc and Men

    Type
    Blog Post
    ...Antibody Resources on Addgene.org  Find recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the...scisignal.aax9730  Additional resources on the Addgene blog Antibodies 101: Monoclonal Antibodies Plasmids...

  11. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...choosing a lab Resources on Addgene.org Find open positions at Addgene Check out our career videos ...neurodegenerative diseases.   Additional resources on the Addgene blog Check out all careers related blog posts ...

  12. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...standardize your experiments later. Stay tuned to the Addgene blog and be sure to check out our viral vector ... PMCID: PMC330758. Additional Resources on the Addgene Blog Learn All about Adenovirus Associated Virus...Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages Find ...

  13. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...back-bone specific primers. Find Empty Backbones at Addgene Each approach has advantages and drawbacks, which...Overview of Colony PCR Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC ...about Gibson Assembly Additional Resources on Addgene.org Read about Choosing a Molecular Cloning Technique...

  14. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...More examples of these biosensors can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be...More Helpful Websites & Resources: FP guide at Addgene Interactive Visualization of Fluorescent Protein...

  15. Genetic Code Expansion

    Type
    Collection
    ...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian...

  16. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...lentiviral-based CRISPR libraries available at Addgene. The large packaging capacity and stable expression...s41392-024-01780-w Additional resources on the Addgene Blog Overcoming the AAV Size Limitation for CRISPR...Using the FITS Approach Additional resources at addgene.org CRISPR pooled libraries Guide to pooled libraries...

  17. Design Tips for Prime Editing

    Type
    Blog Post
    ...programs to help you design pegRNAs. Here on the Addgene blog, we featured a guest blog by the developer...s41586-024-07259-6 Additional Resources on the Addgene blog Prime Editing: Adding Precision and Flexibility... Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by...

  18. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols Viral Plasmids and Resources...Resources and references More resources on the Addgene blog Viral Vectors 101: Producing Your rAAV AAV...

  19. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...Polyclonal Antibodies Resources on addgene.org The Addgene Antibody GuideAddgene's recombinant antibody collection...luck and happy binding! More resources on the Addgene blog Antibodies 101: ELISAsAntibodies 101: Selecting...

  20. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...Nicola Patron, who shared the MoClo plant kit via Addgene. For us, transient leaf transformations are a great...PMCID: PMC2699436. Additional resources on the Addgene blog Read all of our plant biology blog posts ...Read our Plasmids 101 blog posts Resources on Addgene.org Browse our Plant Plasmids and Resources page...
Showing: 801 - 820 of 1091 results