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...rare diseases. These underrepresented individuals deserve a voice. Check back with Addgene often to learn...

...accessibility updates include a printer-friendly design and alt text on all images. We have also made accessibility...

...will need to be accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG...

...PAM sequences required by each ortholog, a gRNA designed for one dCas9 should be specific to that ortholog...

...multiple recombinases can be used together to designate specific patterns of recombination. Finally, Rubin...

...He charged the postdocs to “Grab ahold of your destinies” and also suggested some concrete actions that...

...with genes knocked out in the insulin signaling cascades in only 4 months compared to a year or greater...

...already made for them,” Baird said. “They are all designed in the same vector backbone with very similar ...

...deposited at Addgene by over 250 scientists in Europe. Despite these fantastic numbers, a lot of European scientists...

...entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped away from...

... 1: Buffer exchange using a desalting column The 10 mL Zeba Spin Desalting Columns can accommodate up ...This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using...Western Blot Protocol Introduction This protocol describes how to affinity purify recombinant antibodies ...L2884 Aprotinin, Millipore Sigma A6279 Zeba Spin Desalting Columns, 7 kDa MWCO, 10 mL, Thermo Fisher 89893... buffer exchange using a Zeba Spin 7 kDa MWCO desalting column. If the concentration of the pooled sample...into 2 columns. Twist off the 10 mL Zeba Spin Desalting Column’s bottom closure and loosen cap. Place ...Spectrophotometer to see if the sample has reached the desired concentration. If the sample concentration is still...

...References: del Valle Rodríguez A, Didiano D, Desplan C (2011) Power tools for gene expression and clonal...

...and morale. Especially when your collaboration includes scientists working in different time zones, it...

...distributed more than 100 times. We hope these desktop trophies will serve as badges of honor for our ...

.... Traditional restriction cloning is one of the oldest of these techniques and is a multi-step process...

... with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth. As well as being the Director...

...results. GeoCas9 RNP editing in mammalian cells Besides being able to take some heat, GeoCas9 can introduce...

...These sensors generally follow the same pattern described above - a sensing domain induces changes in a ...

...spontaneous swimming. References Arrenberg, Aristides B., Filippo Del Bene, and Herwig Baier. "Optical...

...plastic is a substantial portion of lab waste. Despite the fact that researchers represent a minority ...