New Viral Vectors - Summer 2024
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Blog Post
...opsin!) and due to its bistable nature, it enables inhibition of synapses distant to the site of injection...
We narrowed to 1,046 results for: sta
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Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service
TypeBlog Post...receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation? Great news...DNA As we begin testing out this service, we’re starting with 210 plasmids from the collection. You can... -
Newly Updated AAV Data Hub!
TypeBlog Post...) can save you significant time and money. For instance, this report shows that anterograde, monosynaptic...required for a DOI. Reports that meet our minimum standards (* above) will be given a DOI for easy citation... -
Plasmid Grant to Aid Cervical Cancer Screening in Ghana
TypeBlog Post...16 and 18.” HPV plasmids as positive controls To start her work, Gladys will improve cervical cancer screening...Plasmid Grant to a third round of applicants at the start of 2017 and would like to thank Seeding Labs for... -
Editor's Choice, October 2016
TypeBlog Post...podcast all about graduate student life and how establishing a thriving graduate student community can make...have tons of great advice for those of you just starting or pushing through your PhDs. Of course there ... -
Grad School Advice Part 1: Picking a Lab and a Project
TypeBlog Post...them difficult to approach for the youngster just starting in the lab, but they are exactly the people you...students are friendly and full of useful advice. Stay tuned for concrete advice on how to pick a lab and... -
Editor's Choice, July 2016
TypeBlog Post...bloggers each and every month, we've decided to start an "Editor's Choice" series. Each month, I'll summarize...for Gene Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo As... -
Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up
TypeBlog Post...mentorship and networking opportunities, publishing assistance, and more to Instrumental Access participants...Learn About Our Recent Visit to China Read Zafira Castano Corsino’s Recent Post on the International Mentorship... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog Post...A newly established all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering.... “However, there had been no systematically established way of making an all-in-one vector for multiplex... -
Plasmids 101: How to Name Your Plasmid in 3 Easy Steps
TypeBlog Post...can usually derive: a) the bacterial antibiotic resistance, b) the promoter that drives the insert, and ...followed by its position (number) relative to the start Methionine (Met) followed by the one letter abbreviation... -
The Addgene Antibody Data Hub
TypeBlog Post... your research, you've probably found yourself staring at a browser full of tabs, each open to a different...anti-Lhx6.1 antibody. For a more detailed understanding of the experimental conditions in which the ... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog Post...several hundreds of nanograms or micrograms of starting material; Gibson assembly or Golden gate assembly...success. In conclusion, it is important for you to establish in advance how you want to utilize your plasmid... -
Inntags: Innovative Protein Epitope Tagging
TypeBlog Post...protein, inntags IT5 and IT6 are a great place to start. These tags are easy to use in multiple common applications...Nature methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and fluorescent-protein... -
Save Time and Money by Making Your Own Competent Cells
TypeBlog Post...you're transforming a plasmid with ampicillin resistance. After a plasmid is introduced to bacteria, ...culture without antibiotics to give the antibiotic resistance gene time to be expressed. However, you can ... -
Microbes: Look, Listen, and Tell
TypeBlog Post...microscope called the ‘foldscope’ (Dr. Prakash, Stanford University). Right there on the floor outside ...Microbiology and Fluorescent Protein Resources and get started! I learned a lot about creative ways to do science... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog Post...Design: FRET reporter activity depends on both the distance and the orientation of the donor and acceptor ...of the nucleus depending on its phosphorylation status. The relative ratio of cytoplasmic vs. nuclear ... -
Addgene's Top Blog Posts from 2020
TypeBlog Post...plasmids in one cell, it might be that you can't stably maintain them in the cell. This blog post features...blogger Shravanti Suresh who studies CRISPR at Iowa State University detailed five methods for using CRISPR... -
Designer PUF Proteins for Any RNA Target
TypeBlog Post... a couple of tips: First, she recommends using standard BsaI in place of the high fidelity version (despite...investigating the issue and hopes to have a better understanding of the phenomenon soon. Rest assured, as the... -
Why Do I Need an MTA Anyway?
TypeBlog Post... We understand - you’re a busy scientist working hard on research, and you need the right experimental...Have a question? Our friendly and knowledgeable staff is here to help. Contact us at [email protected] or... -
Editor's Choice, September 2016
TypeBlog Post...contributed by guest blogger Sean Stacey from Eastern Tennessee State University and provides a brief rundown...