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...annual tradition in which we choose our teams and start brainstorming months in advance. Our teams comb ...into an outfit?), and of course there’s always nostalgia (old board games and movies can provide great ...

...receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation?  Great news...DNA As we begin testing out this service, we’re starting with 210 plasmids from the collection. You can...

...opsin!) and due to its bistable nature, it enables inhibition of synapses distant to the site of injection...

...) can save you significant time and money. For instance, this report shows that anterograde, monosynaptic...required for a DOI. Reports that meet our minimum standards (* above) will be given a DOI for easy citation...

...16 and 18.” HPV plasmids as positive controls To start her work, Gladys will improve cervical cancer screening...Plasmid Grant to a third round of applicants at the start of 2017 and would like to thank Seeding Labs for...

...podcast all about graduate student life and how establishing a thriving graduate student community can make...have tons of great advice for those of you just starting or pushing through your PhDs. Of course there ...

...them difficult to approach for the youngster just starting in the lab, but they are exactly the people you...students are friendly and full of useful advice. Stay tuned for concrete advice on how to pick a lab and...

...bloggers each and every month, we've decided to start an "Editor's Choice" series. Each month, I'll summarize...for Gene Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo As...

...mentorship and networking opportunities, publishing assistance, and more to Instrumental Access participants...Learn About Our Recent Visit to China Read Zafira Castano Corsino’s Recent Post on the International Mentorship...

...A newly established all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering.... “However, there had been no systematically established way of making an all-in-one vector for multiplex...

...can usually derive: a) the bacterial antibiotic resistance, b) the promoter that drives the insert, and ...followed by its position (number) relative to the start Methionine (Met) followed by the one letter abbreviation...

... your research, you've probably found yourself staring at a browser full of tabs, each open to a different...anti-Lhx6.1 antibody.    For a more detailed understanding of the experimental conditions in which the ...

...several hundreds of nanograms or micrograms of starting material; Gibson assembly or Golden gate assembly...success.  In conclusion, it is important for you to establish in advance how you want to utilize your plasmid...

...protein, inntags IT5 and IT6 are a great place to start. These tags are easy to use in multiple common applications...Nature methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and fluorescent-protein...

...you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to bacteria, ...culture without antibiotics to give the antibiotic resistance gene time to be expressed.  However, you can ...

...microscope called the ‘foldscope’ (Dr. Prakash, Stanford University). Right there on the floor outside ...Microbiology and Fluorescent Protein Resources and get started!  I learned a lot about creative ways to do science...

...Design: FRET reporter activity depends on both the distance and the orientation of the donor and acceptor ...of the nucleus depending on its phosphorylation status. The relative ratio of cytoplasmic vs. nuclear ...

...plasmids in one cell, it might be that you can't stably maintain them in the cell. This blog post features...blogger Shravanti Suresh who studies CRISPR at Iowa State University detailed five methods for using CRISPR...

... a couple of tips: First, she recommends using standard BsaI in place of the high fidelity version (despite...investigating the issue and hopes to have a better understanding of the phenomenon soon.  Rest assured, as the...

... We understand - you’re a busy scientist working hard on research, and you need the right experimental...Have a question? Our friendly and knowledgeable staff is here to help. Contact us at [email protected] or...