... alternative cloning strategies There are many strategies beyond the traditional PCR/digest/ligate methods... production genome wide screens and the more traditional “one at a time” approach. According to his math...

...raised or hours tracked, 3% of postdocs were at institutions focused only on tracking hours, and 22% of...that institutions have sent, with the lack of transparency and poor communication of their plans (many ...postdocs at the University of California, who had a contract - negotiated by their union - securing higher ...

...in the US and Western Europe have taken three strategic approaches to gender equality over the past several...an international research team from Norway and Australia worked with stem cells in mice. They appropriately...female stem cells—this was an unconscious and arbitrary decision. Unfortunately, it also meant that, by... Scientific Impact Are Evoloving Check Out Extracurricular Activities for a Strong Science Career Path...

...will allow you to set the amount of transcriptional and translational activity a cell has and control how...actually were not many hurdles. It was pretty straightforward surprisingly. So I think a nice thing about...on NOR gates. It’s a fundamental unit. We did demonstrate that you can use the CRISPR chemistry for a NOR...

...tools used were key for ultra-high-complexity assembly. While the traditional rules of thumb for overhang...Potapov et al., 2018), which showed that the five traditional rules didn’t need to be strictly followed in ...sets (with overhangs that violate some of the traditional design rules) for successful high-complexity ...

...that the TadA domain can edit the short single-stranded DNA stretch that is accessible. Image from Gaudelli...used a previously developed bacterial selection strategy (Gaudelli et al., 2017) in combination with a ...dC9-ABE8.x-m/d). The dC9-ABE8.x-m/d constructs demonstrated a 2.1x on-target DNA-editing efficiency in a...attribute for therapeutic application. ABE8s demonstrate an overall improved base editing capacity, even...

...which was trained on key factors from past PubMed user searches, such as clicks to read the abstract or full...publications and figures using machine learning to extract important features such as influential citations...the Chan Zuckerberg Initiative. Meta is not a traditional search engine. Instead, you create your own research...

...Gate cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the Fragmid system...AAV pRDA_889 (ITRs from AAV2) Mammalian Transposon pRDA_757 (Piggybac; constitutive expression...   Drosophilia Plasmid pRDB_053 (for transgenic flies; gypsy insulator sequences; white + selectable... drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector (dropdown options...

...need for gene editing in research, the old PAM constraints just won’t cut it anymore. Say hello to PAM-free... evolve one that uses a PAM with no sequence constraints, or at least has looser or different requirements.... The activity of SpRY was validated in double stranded cleavage and base editing applications, with high...Plasmids available here) Walton, R. T., et al., Unconstrained genome targeting with near-PAMless engineered...

...constantly moving so we need good ways to monitor and track them. We have a number of microscopes, including...meaning that we can obtain known mutants at $7.00 a strain. Makerspaces like ours can also make accounts with...motor behaviour, and ultimately submit our new strains back to the CGC for other researchers to use. Although...money is always tight. Of course, unlike your traditional biology lab, we have members with a wealth of...

...contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers Keep an eye on the ...too young and should not be used for important transfection experiments right after they’ve been thawed....9(6): p. e98635. PubMed PMID: 24901218. PubMed Central PMCID: PMC4047077. 2. Pagliara, Patrizia, et al...

... approaches (Vu et al., 2024). Their optimized strategy uses new combinations of PE protein components...NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited an edited...then used single-molecule FRET to study the intramolecular dynamics of the proteins in solution and when...-dependent and -independent versions that are trafficked to the soma via the Kv2.1 targeting motif as ...

... because the system modulates protein post-translationally, unlike many other methods. Secondly, these...proteins. Thirdly, degrons and their drugs are titratable. With these tags, protein levels aren’t limited...limited to ‘on’ and ‘off’; varied levels of drug concentration can yield intermediate protein abundance. Finally... like the SMASh tag does. However, it is a straightforward system – HaloTag and its PROTAC are all that...

...shown them how to isolate DNA from a strawberry, or demonstrated that they’re living in an exciting, tumultuous... think that establishing this history helped demonstrate that a) I was a real person who used to be a ...become public in the previous two weeks, which demonstrated to the students that scientific history was ...

...Scientists Making It Happen, is an exercise in restraint. The very first subheading in Chapter 1 asks “..., and he wisely makes no such attempt. He is transparent about his own biases--many of which will be shared...health and environmental science, by the Trump administration are difficulties in dealing with climate change...dire than Zimmer could have imagined. Does this detract from the quality of the book? It doesn’t need to...

...connectivity to open source data repositories. To illustrate this, let’s take a look at the RSpace ELN.  The... commonly used online file storage tools, as illustrated in this video, and to data stored on institutional...is the integration between RSpace and Slack, illustrated in this video. This is a two-way integration....protocols.io, the protocol sharing repository. As illustrated in this video the integration enables RSpace ...

...drafts of the manuscript. This is the time to transform the document into something cohesive-- change ...This is normal and it means you’re on the right track!  Get feedback After you’re done editing, send it...Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell ...6.5-6 (2015): 184. PubMed PMID: 26124919. PubMed Central PMCID: PMC4482241. Additional Resources on the ...

...practice. I always take a poll of scientists in training to see how many in my audiences have given a 50...prepare answers Speaking pros always have a few extra slides in their back pocket. During your practice...else have a good answer for this?” This doesn’t detract from the speaker, but actually makes for a more...session. The best speakers use no notes and can concentrate on delivery, rapport with the audience, and their...

...While this may effectively serve as a transfection or transduction control, a different gRNA would have... Lowering off-target events There are several strategies to lower the predicted frequency of off-target...near each other, this will result in a DNA double strand break (DSB), the event required for frameshift ...

...science, and maybe even win a $500 travel prize in our open data extravaganza!  Anyone can submit data from...presentation, with two winner each receiving a $500 travel award. All participants will receive an Addgene...