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We narrowed to 960 results for: ARC

Showing: 411 - 420 of 960 results

  1. An Introduction to Adenovirus

    Type
    Blog Post
    ...between early and late gene transcription) is demarcated by viral genome replication (Harrach, 2014). ... majority of adenoviral vector systems used in research, they are only expressed in the producer cells... to standard uses in gene therapy and in basic research, like other viral vectors, though significant ...inspires further use of existing vectors, as well as research into the development of new adenoviral vectors...., Bazinet, A. L., Bergman, N. H., Bull, R. L., Garcia, K. Y., Goodrich, J. S., Lovett, S. P., Parker,...

  2. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...non-living viral particle, and how we as dedicated researchers can exploit it. This blog was written by Addgenie...10.3390/v13071288. PMID: 34372494. Prussin, A. J., Garcia, E. B., & Marr, L. C. (2015). Total Virus and Bacteria.... The nucleic acid of the Bryan strain of Rous sarcoma virus: purification of the virus and isolation ... G. M., & Temin, H. M. (1974). Infectious rous sarcoma virus and reticuloendotheliosis virus DNAs. Journal...M. (1973). RNA-directed DNA polymerase of Rous sarcoma virus: Initiation of synthesis with 70 S viral ...

  3. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...want to highlight it for use in your research. Martínez-García et al., BMC Microbiology 2011 Feb 22...progression. One of the major challenges in signaling research is the inability to replicate the spatio-temporal... post-translational modifications in cells and research into its many roles in protein function and signaling...at Yale University previously changed the way researchers can explore important questions surrounding serine...Nicola Patron's MoClo kit and her plant synbio research, read the Addgene interview. Engineering TALENs...order to extend the utility of the system, the researchers developed three new versions. Flipbow employs...

  4. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...types in the sample. The visualization helps the researcher see which of the multiplexed sgRNA was involved...browser’s Control+F or Command+F functionality to search for a particular sequence or name. You can perform...educating and supporting novice to expert-level researchers in achieving consistently successful CRISPR experiments...enthusiast, and when she is not guiding CRISPR researchers, she's either day-dreaming about exploring sci-fi...our CRISPR guide Find CRISPR plasmids for your research Find other CRISPR software resources ...

  5. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...selection. In a subcutaneous cancer model, the researchers showed that after 24 hours, the unstabilized ...in nature, these systems have been adapted by researchers for many uses when plasmid retention without ...for long-term experiments. They’ve also helped researchers design and optimize cloning methodologies using...genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed PMID: 19416369. Kulakauskas....org Check out our molecular biology reference Search for a plasmid here Find plasmids from our special...

  6. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ... contributed by Jacob Lazarus, a postdoctoral researcher at Harvard. There’s an astounding number of ways... flexneri, Enterobacter cloacae, and Serratia marcescens and have created dozens of deletions as well ...attending physician. He performs post-doctoral research in the lab of Matthew Waldor at Harvard. Now that...sliding at upstream standby sites." Nucleic acids research42.4 (2013): 2646-2659. PubMed PMID: 24234441. PubMed... unmodified Enterobacteriaceae." Nucleic acids research 43.13 (2015): e83-e83. PubMed PMID: 25800749. ...

  7. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...from Ludwig-Maximilians-University in Munich and Marc Tessier-Lavigne’s lab at the Rockefeller University...clones! We would therefore strongly encourage any researcher to incorporate CRISPR/Cas-blocking mutations ...Neurobiology at the Institute for Stroke and Dementia Research, Ludwig-Maximilians-University Munich. His lab... the described project while he was a Postdoc in Marc Tessier-Lavigne’s lab at the Rockefeller University...University in NYC.   Dylan Kwart is a graduate student Marc Tessier-Lavigne’s lab at the Rockefeller University...

  8. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...expression or silencing. To alter DNA modifications, researchers used blunt tools like histone deacetylases, but...epigenetic modifications at a user-specified locus. Researchers showed that TALE-TET1 constructs, which fused...transcription at CpG regions of various promoters. Other researchers additionally fused a TAL effector to LSD1 histone...and dCas9-based epigenome editing. Nucleic Acids Research 47:e23–e23. https://doi.org/10.1093/nar/gky1286...system for targeted DNA methylation. Nucleic Acids Research 44:5615–5628 . https://doi.org/10.1093/nar/gkw159...

  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...developed by the GENIE Project team at HHMI's Janelia Research Campus (Aggarwal et al., 2025).In this latest ...genetic cargo of choice. After hearing about researchers' favorite features and limitations of existing...and striatal interneurons (Furlanis, Dai, Leyva Garcia et al., 2025).More than a dozen of these enhancer-AAV...cell morphology. Adapted from Furlanis, Dai, Leyva Garcia et al. 2024 bioRxiv, under a CC-BY-NC-ND 4.0 International...from the article! Furlanis, E., Dai, M., Leyva Garcia, B., Tran, T., … Dimidschstein, J., Fishell, G....

  10. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...the complexity of the system, researchers have used split GAL4 architecture or combined the system with ... ON state, and how you might use these in your research. Today, we’ll look at repressible promoters, which...bind the promoter and repress pADH1 activity. Researchers have engineered pADH1 variants that are less ...(2006). https://doi.org/10.1186/1472-6750-6-43Kaczmarczyk A, Vorholt JA, Francez-Charlot A. Cumate-inducible...
Showing: 411 - 420 of 960 results