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We narrowed to 1,085 results for: ANT

Showing: 821 - 840 of 1085 results

  1. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ... organisms: mammalian cells, yeast, drosophila, plants, worms, zebrafish, frogs, some bacteria; but not...has maximum activity from 50-70 °C. The key advantage of GeoCas9 is that it functions at temperatures...yet? Let us know in the comments below. And if you want to give GeoCas9 a try, as always, the plasmids are...

  2. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...worldwide and the presentation made it clear how important it is for us to improve our resources for this... meet hundreds of the over 11,000 conference registrants, including scientists from six continents who...presentation at the CRISPR screens symposia outlined the fantastic work this Broad Institute lab is doing creating...

  3. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...invading phages. Rising to the challenge - Evolution of anti-CRISPR genes in phages The CRISPR/Cas adaptive immune...CRISPR/Cas. Earlier this year, expression of 1 of 5 anti-CRISPR genes were found to inactivate the Type I-F... the presence of a variety of undiscovered viral anti-CRISPR genes. These data highlight an interesting...

  4. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...teenager in high school, I knew that this is what I wanted to do,” Stegmeier says He began this journey in...wealth of resources on Addgene’s website and blog important. “The experience from the scientific community... either through direct industry collaborations, grants, or setting up commercial and nonprofit companies...

  5. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ... of the main tenets of our quality control (QC) mantra is to do our utmost to ensure that scientists are... containing just the expression cassette that we want, it also includes a small amount of spurious DNA...packaging plasmids. We decided to use this to our advantage by designing a Python script that looks for pre-defined...

  6. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...I didn’t truly address the question of what I wanted to do when I grew up until I was all but finished... I was struck with the realisation that I didn’t want a career in neuroscience—whatever that entails. ... this subject allowed me to engage in findings relevant to my background (neuroimaging, biomechanics, ...

  7. Viral Vectors 101: Transductions

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    Blog Post
    ...is the biological outcome you want to achieve? For example, do you want the nucleic acid cargo to be integrated... If you know the basics but are here because you want to know the intricacies of transductions, we've ...

  8. CRISPR Challenges: Standardization and Homology Directed Repair

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    Blog Post
    ...evaluating CRISPR technologies, including the ever important on/off-target efficiency. Likewise, the NIST Genome... template that contains your desired edit, and I want to make sure that I (and Addgene) don’t forget this...Check out this post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as...

  9. The Strength of Story Telling

    Type
    Blog Post
    ...enjoy, remember, and listen to narratives — take advantage of that in your work The part of my job I most...add author details a different person in the lab wanted to be the first author. When I raised this, he ... likely to be memorable. Work out what story you want to tell; tell it; stop. Many thanks to our guest...

  10. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ...cultures in the shakerTurn the LEDs onAnd when you wantYou can just turn them offNo need for any washNo need... aureochrome1 (VfAu1), led to functional BLADE variants, too. The team tested different linkers between...light without the need of any cloning.     What advantages does BLADE have over AraC? When compared to the...

  11. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different pH’s and some that are...School describes how AAVs function and indicates important things to consider if you’re getting started with...through Addgene.  Thanks again to all of our fantastic depositors and guest writers. Your contributions...

  12. Reagent Repositories Are Speeding up Science During the Pandemic

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    Blog Post
    ...SARS-CoV-2 were developed for broad use and are now important for accelerating research into this virus. One...scientific community. Addgene was awarded a Fast Grant to ensure that the scientific community will continue...Deposits of new reagents, policies from journals and granting agencies requiring materials to be deposited into...

  13. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...CRISPR tools, without compromising their strategic advantages in gene editing, while keeping the system to ... array with a given tRNA (derived from human or plant source), by tuning the length of 5’ leader sequences...outperformed other tRNA candidates (either from human or plant sources) in both the strength as a promoter and ...

  14. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment.  ...Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but much of...valid email address).  One may also simply attach important references that serve to further characterize ...

  16. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...screen and transform your work into a paper or a grant. Here’s a list of tools and resources that might...differences between these two citation managers. If you want more tips on finding and organizing papers, check...post: Early Career Researcher Toolbox: Finding Relevant Papers. These are only a few of the many writing...

  18. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... worthwhile. Particularly if in the end all they want to do is to quickly generate a single cell line ...." If you ever run out of ready-to-use virus and want to make new lentiviral preps on your own, fear not...excited to provide this new service for researchers wanting an easier way to use CRISPR and would love to hear...

  19. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...be of secondary importance (or tertiary, or non-existant). On the very first night of the iPSC symposium... the stem cell community for addressing these important questions that the scientific method cannot address... it took the joint effort of five post-docs to implant human kidney progenitors into a pig. In stark contrast...

  20. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...get more done. There are also a host of other advantages to practicing effective delegation skills: ...responsibility if things go wrong. Here are some important criteria for delegating tasks appropriately: ... and accountability? What types of issues do you want to know about right away? Provide adequate support...
Showing: 821 - 840 of 1085 results