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Showing: 821 - 840 of 1030 results
  1. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls like single color...
  2. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...Resources Find CRISPR Plasmids for Your Research Catch up on Your CRISPR Background with Our Guide Pages...
  3. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ...Find Ready-to-Use Lentivirus Find Ready-to-Use AAV Catch up on Chemogenetics ...
  4. Antibodies 101: Isotypes

    Type
    Blog Post
    ... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...of these five isotypes, such as the number and location of disulfide bonds, the number of constant domains...
  5. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...insert. All colonies on this plate should be blue, indicating that your IPTG and x-gal are working as they ...of false positives: Blue-white screening only indicates the presence of AN insert, not necessarily YOUR...
  6. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...radioactivity in the culture supernatant (this is indicative of protein degradation). To control for protein...studying autophagy. This method relies on the identification of autophagic structures based on morphology...
  7. All in a Twist: dsRNA

    Type
    Blog Post
    ...generate dsRNA as part of their infection and/or replication process within the host. dsRNA viruses are inherently...generate dsRNA intermediates as part of the viral replication process. dsDNA viruses yield dsRNA through a ...
  8. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ... example, I will often see a whole bunch of notifications “a postdoc from this Uni viewed your profile...look straight at the camera. Pro tip: Turn off notifications to your network of profile changes. If you are...
  9. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...might even be expected to write up a report to communicate your findings to your colleagues. So perhaps ...about the issue, assessed potential protocol modifications, and ultimately decided on the changes to implement...
  10. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...AsCas12f1. They found that this system, without any modifications, is effective in genome editing in both bacteria...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease....
  11. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...for a scaleable lab. When you need to track the location, quality, growth, and legal status of thousands...stab culture Ship the stab culture to the proper location To keep ourselves from mixing up samples and ...
  12. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    .... To remedy this, Shah et al. provide a table indicating the sex of the most commonly used cell lines ... work to be done! Researchers need to learn sophisticated methods of sex and gender analysis. Universities...
  13. Working with Nuclear Receptors

    Type
    Blog Post
    ...murine members. NRs permit the integration and communication of such signals between central and peripheral...macrophage activation. Our mass spectrometry-affinity purification proteomic profiling of coregulators in HeLa ...
  14. Lab Automation at Addgene

    Type
    Blog Post
    ...efficient. Here, I’ll discuss transformation, DNA purification, and filling vials with agar. I’ll also provide...greater than 95% success rate! Automating DNA purification We're developing ways to prep large quantities...
Showing: 821 - 840 of 1030 results