Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 1,149 results for: Gene

Showing: 821 - 840 of 1149 results

  1. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...animation is becoming a TikTok series! Follow @addgene on TikTok for bite-sized portions of antibody concepts...an antibody?" or "what is an IgE isotype?"  @addgene Ready to learn about antibodies? Our new antibodies...molecularbiology #science #scicomm ♬ original sound - Addgene We'll be posting these animations regularly ... all of our videos will also be shared on our @addgene Instagram account, or you can watch the full animation...on our YouTube channel.  More resources on the Addgene blog Antibodies 101: Introduction to Antibodies...

  2. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    ...program are here. This was a great opportunity for Addgene to present our own data on plasmid deposits and...and distirbution for these fast moving fields.  Addgene is a global nonprofit plasmid repository. Over ...Over 2,000 labs have deposited plasmids to Addgene and we distribute over 130,000 plasmids in 2014. Thus,...combination of open-source distributors such as Addgene, as well as a number of online user forums such...Meeting Eric presented a poster with data comparing Addgene global deposit and distribution trends for Zinc...

  3. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase...edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A... the desired edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically...peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does...dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...

  4. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...work is done in flow cytometers, and output is generated through measurement of events. An event is something... machine is plotted as a dot on an X,Y axis to generate a dot plot (Figure 1). Clustered events are called...                         Figure 1: While the plot generated often looks like the graph on the left, it might...intersection of X,Y. For flow plots, however, that is generally not true. Because of the variations in fluorescent...Of course, this is quite easy to do on a plot generated in Biorender! While a helpful visualization tool...10.1371/journal.pone.0137550 More resources on the Addgene blog Conventional vs. Flow Cytometry Plasmid-based...

  5. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts  ...Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ...that interact with nucleic acids. Labs studying epigenetics routinely use the chromatin IP (ChIP) technique... found this blog helpful be sure to check out Addgene’s blog for other antibody topics.     References...

  6. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...open access journals.   Check Out Addgene Videos Over at the Addgene Youtube Channel   After watching ...what online tools people generally like to use to share ideas – with the general goal of seeing if and how...Communication 2.2 (2014): 2. Additional Resources on the Addgene Blog 6 Tools & Tips: Online Social Networking ...Sharing in Research Publications Resources at Addgene.org Check out our Protocol Pages for Video Protocols...

  7. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything... sales-y in posts which falls in line with our general mission to share- as opposed to sell- science. ...serious career dilemmas. Some questions were about general advice on how to meet people, how to transition...content on many of the requested topics to the Addgene blog The blog has many other excellent posts on...and happy to help. Additional Resources on the Addgene Blog: 10 Ways to Share Your Science 6 Tools & ...How We're Sharing Science through Video with the Addgene Youtube Channel Check Out Vidoes of Gender & Science...

  8. The Challenges of Cell Culture

    Type
    Blog Post
    ...pharmacology (many of which are represented in Addgene's Special Collections). Arguably, oncology has benefited...examining whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique...out by the commercial repository. Repositories generally ensure cell line identity and purity making it...to the original as possible and so any results generated are reproducible and reliable. When you order ...Abcam Cell Culture Guidelines Resources on the Addgene Blog Learn All about Mammalian Vectors Read up...Using GFP for Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...

  9. Genetic Code Expansion

    Type
    Collection
    ...EcTyrRS/tRNA pair (tyrS gene knocked out, tyrTV gene replaced by tolC, and tyrU gene replaced by GentR). ...EcTyrRS/tRNA pair (tyrS gene knocked out, tyrTV gene replaced by tolC, and tyrU gene replaced by GentR) and...Removed endogenous EcTyrRS/tRNA pair (tyrS gene knocked out, tyrTV gene replaced by tRNA variant that suppress...endogenous EcTyrRS/tRNA pair (tyrS gene knocked out, tyrTV and tyrU genes replaced by tRNA variant that suppress...endogenous EcTyrRS/tRNA pair (tyrS gene knocked out, tyrTV and tyrU genes replaced by tRNA variant inactivated...that contain genes with modified codons for unnatural amino acid incorporation. ID Plasmid Gene/Insert Vector...non-canonical amino acids (ncAAs). A plasmid containing the gene of interest with the modified codon (typically the...

  10. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...engineered ascorbate peroxidase (APEX), used as a genetic tag for electron microscopy, could be used for ...(Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the development of BioID and APEX, optimized...These newer tools are also currently available at Addgene.  BioID2: A smaller improved promiscuous biotin...utilized yeast display-based directed evolution to generate two mutants, the 35 kD TurboID, which has 15 mutations...et al. "Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy." Nature...PMCID: PMC5467174. Additional resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101...

  11. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...partnered with Addgene to make our collection of integrin antibodies available through Addgene’s repository...10.1038/s41467-022-35173-w More resources from Addgene Addgene's Antibody Guide Antibodies 101: Flow Cytometry...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...on these cells. What’s next IPI is working with Addgene to accelerate biomedical research by distributing...

  12. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...Science Communication Resources at Addgene.org Check out Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...small tasks (like... preparing plasmids to send to Addgene). Following my advice from above, you should probably...appropriately. Back in my undergrad days, I had general goals for my experiments, but didn’t spend enough... in the comments! Additional Resources on the Addgene Blog Management for Scientists eBook Get Advice...

  13. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...remotely instead of from the lab. Nonetheless, Addgene’s annual Deck the Lab competition drew upon the ...Decoration Competition! #ScienceFun #ZoomParty @Addgene pic.twitter.com/drNBUdlQco — The Tarling-Vallim...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com...description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com/GsAlD6sjM6 — Dr. Anne A Madden ...thank you to everyone who entered. Keep an eye on Addgene’s social media throughout the year for more fun ...

  14. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    ...ways of generating antibodies, for example through the use of less toxic adjuvants; 2) generating complex...and 4) generating mAbs against new cancer biomarkers. We believe that repositories, such as Addgene and ...The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years Data Freedom: The Expansion of...

  15. Bacterial Expression Systems

    Type
    Collection
    ...knocking out genes or introducing specific mutations can reveal important insights into gene function. These...expression of a gene of interest in E. coli . The Marionette Sensor Collection (Addgene #1000000137) allows...of up to twelve genes using small-molecule inducers in the same E. coli strain. Addgene Blog Inducible ...bacterial gene biology or engineering metabolic pathways to synthesize a target metabolite, genetic tools ...visualization, study of protein interactions, controlled gene expression, reporter plasmids and genome engineering...transcriptional regulatory elements that will turn on gene expression upon protein–protein interaction. The...always advantageous to constitutively express your gene of interest at high levels. Perhaps high expression...

  16. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... anti-CRISPR genes in bacteriophages infecting Pseudomonas aeruginosa . Anti-CRISPR genes employ varied...especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are important...system - utilizing various CRISPR-associated (Cas) genes to not only store a record of invading phages but...Csf1) Type VI (Cas13) Fighting Back: Anti-CRISPR Genes in Phage The CRISPR adaptive immune system seems...produces insertions/deletions disrupting a target gene. On the other hand, if a repair template with homology...according to this template, allowing for precise gene editing. These edits can include mutations seen ...given phenotype. Current libraries are available for gene knockout, as well as transcriptional activation ...

  17. A Control for All Seasons

    Type
    Blog Post
    ...Selecting the Right Antibody Resources on Addgene.org Addgene's Antibody Guide  ...controls for biological experiments, starting with general controls and then moving on to controls for antibody-related...interpretation of results. A positive control is generally a sample or group that will have a desired response...address this, block positively charged groups with generally sticky proteins such as bovine serum albumin. ...Central PMCID: PMC2712964. More resources on the Addgene blog Antibodies 101: Validation Antibodies 101:...

  18. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...is a single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...please let us know! Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

  19. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...into lentiviral vectors to generate a kit which is now available at Addgene. “We called it cloning by ...growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs/ Finding...PMCID: PMC4353587. Additional Resources on the Addgene Blog Learn How the Clon Tracer Library Has Been...Technologies from 2015 Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled...

  20. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...Internship with Addgene, I didn’t know a lot about scicomm, but had enjoyed writing a few Addgene guest blog...careers here Resources on Addgene.org Learn more about careers at Addgene ...Twitter @HansPacker. Additional Resources on the Addgene Blog Get tips on how to do your own informational...
Showing: 821 - 840 of 1149 results