We narrowed to 911 results for: IRE

1. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   ...mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs from Bryan Roth’...

2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   Blog Post

   ...Although this method shortens construct size, it requires coexpression of Csy4 with Cas9 and the polycistronic...

3. How to Make Friends and Meet People at a Scientific Conference

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   Blog Post

   ...the person you are talking to is really in your direct area.  Review the conference schedule and attendance...

4. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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   Blog Post

   ...is helpful to find peers that share a similar direction or problem. For example, a group of people who...

5. Transferable Skills Guide: Public Speaking

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   Blog Post

   ...collaboration and growth opportunities, and gives your entire audience a chance to comfortably engage with you...

6. Giving gRNAs a Facelift - Synthetic and Beyond

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   Blog Post

   ...chemical modifications to the RNA to control the entire editing system with a simple LED light. Lighting...

7. Transferable Skills Guide: Cross-team Communication

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   Blog Post

   ...not so simple Just as our lab used jargon that required context to understand, I came to realize that ...

8. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   Blog Post

   ...endothelial cells from women who are missing an entire X chromosome (TS iPSCs), the lab observed abnormal...

9. Plasmid Tools for Microbiome Studies

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   Blog Post

   ...transfer origin as well as a selectable marker and a desired genetic payload (ex: GFP). Thus, cells can be both...

10. dTAG - You're it!

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    Blog Post

    ...Models CRISPR 101: Cas9 Nickase Design and Homology Directed Repair Plasmids for endogenous gene tagging in...

11. Addgene: The First Twenty Years

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    Blog Post

    ...to be young again! Addgenie Marcy (now our Sr. Director of Production) pie-fully celebrating Addgene's...

12. Antibodies 101: Conjugation

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    Blog Post

    ... 2012). And, you can also conjugate antibodies directly to beads for purification purposes.  Coupling...

13. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    Blog Post

    ...it would take years to develop the mouse lines required for this purpose. Tang wondered if he could use...

14. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    Blog Post

    ...Scientific Manuscripts. Any scientific manuscript will require numerous other references to scientific literature...

15. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    Blog Post

    ...GFP.   Figure 2: Synthetic promoters can be paired for targeted expression. This same pairing of...

16. Early Career Researcher Toolbox: Finding Relevant Papers

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    Blog Post

    ...This is a great way to have new papers delivered directly to your inbox. Best uses Staying up-to-date on...

17. Corralling and Cornering the Goldilocks Antibody

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    Blog Post

    ...perform differently over time), compatible with the desired applications (such as immunohistochemistry (IHC...

18. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    Blog Post

    ...al., 2019). Finally, scientists can perform the entire process of antibody selection and maturation in...

19. Making CRISPR Plasmids Using Fragmid

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    Blog Post

    ... order. (It’s also why you can’t skip a module entirely, but instead will have to replace it with a spacer...

20. Tips for Technical Support Calls

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    Blog Post

    ...end of that email you’re writing is probably not directly responsible for the problem. We’re here to help...