We narrowed to 1,018 results for: LEA
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TypeBlog Post...multiplexing gRNAs here). Cas9 is commonly tagged with a nuclear localization sequence to enhance targeting to the...plant species or cell type (Belhaj et al., 2013). Nuclease dead Cas9 (dCas9) based activators (such as dCas9...
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Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...(7). This modification allowed the deletion of release factor 1, the protein responsible for terminating...reference and protocols for use of this system, please reference ‘Rinehart Lab reagents for improved expression... -
Plasmids 101: Repressible Promoters
TypeBlog Post...fungus Neurospora crassa. The QUAS promoter is less leaky than UAS in the basal state, and co-expression of...expression. Co-expression of repressor QS along with QF leads to intermediate expression from QUAS, as seen with... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...Mentoring Program Director of Strategic Initiatives. Learn more at https://www.linkedin.com/in/lopezdiegothinkbrain...lopezdiegothinkbrain Other Resources Visit the IMP website Learn about the Mentor Circle Program at Brigham and Women's... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post...time investment. Once the target mRNA is cleaved, the cleaved mRNA can induce further rounds of RNAi. ... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...References 1. Hochedlinger, K. and R. Jaenisch, Nuclear reprogramming and pluripotency. Nature, 2006. 441... Factors that Differentiate the Stem Cell Field Learn how lentiviral vectors can be used for gene delivery... -
Antibody Validation for Flow Cytometry
TypeBlog Post...used in this approach should also allow for the clear identification of the cell types of interest. By...Kolahdouzan, M., Chen, C. X., You, Z., Benaliouad, F., Gileadi, O., McBride, H. M., Durcan, T. M., Edwards, A.... -
New Antibody Animation!
TypeBlog Post...Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what ... -
Plasmids 101 eBook 4th Edition
TypeBlog Post... to take our word for it! “When people want to learn about plasmids, they don’t go to Wikipedia; they... -
Introducing...Addgene Quick Tips!
TypeBlog Post...fundamental technical lab skills, like pipetting! We’ll release a new tip every two weeks during the school year... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog Post...second edition includes new and updated content in a clean and easy-to-navigate design. You can find it, along... -
Antibodies 101 Series on TikTok
TypeBlog Post... "what is an IgE isotype?" @addgene Ready to learn about antibodies? Our new antibodies animation has... -
Addgene’s First AAV Data Hub Challenge
TypeBlog Post...burning AAV questions answered. Interested? You can learn more here - and remember, submissions are due Nov... -
Help Us Name Blugene’s New Virus Friend
TypeBlog Post...here Additional resources on the Addgene blog Learn more about Blugene's make-over Get a how-to guide... -
CRISPR Guide Update
TypeBlog Post...BioRender.com. Figure 1: PRIME editing Please check out the updated guide for a great introduction... -
Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!
TypeBlog Post...episode of our Hot Plasmids podcast series, you'll learn about new libraries for studying B. subtilis biology... -
Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More
TypeBlog Post...episode of our Hot Plasmids podcast series, you'll learn about new red fluorescent proteins, AAV tools for... -
Addgene is Stopping Use of X (formerly Twitter)
TypeBlog Post... nonprofit. Recent remarks and actions by X’s leadership conflict with our values. For this reason, our... -
The 12 Days of CRISPR: 2021
TypeBlog Post...programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR, Addgene gave to ... -
CRISPR 101 eBook...en español
TypeBlog Post... Denise Roche, to edit the translation. We are pleased to announce that CRISPR 101: Una herramienta de...