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We narrowed to 1,015 results for: LEA

Showing: 821 - 840 of 1015 results

  4. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...used in this approach should also allow for the clear identification of the cell types of interest. By...Kolahdouzan, M., Chen, C. X., You, Z., Benaliouad, F., Gileadi, O., McBride, H. M., Durcan, T. M., Edwards, A....

  5. CRISPR Guide

    Type
    Guide
    ...like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...target cleavage, while mismatches toward the 5′ end distal to the PAM often permit target cleavage. Generating...binding. In this formation, its nuclease domains, RuvC and HNH, cleave opposite strands of the target ...popular Cas endonuclease, with many modified versions engineered to increase specificity and nuclease activity...Cas9 The two Cas9 nuclease domains, RuvC and HNH, can be altered to reduce cleavage events to a single...contains mutations in both nuclease domains, D10A and H840A, resulting in no cleavage of target DNA. Cas9n ...increase nuclease fidelity SpCas9-NG - NG; increase in vitro activity SpG - NGN; increase nuclease activity...

  6. Cloning

    Type
    Guide
    ...molecular biology enzymes: 5' exonuclease, polymerase and ligase. 5' exonuclease digests the 5' end of dsDNA...properties of type IIS restriction endonucleases. These endonucleases cut dsDNA at a specified distance...) relies on the 3'-5' exonuclease activity of T4 DNA polymerase. An exonuclease is an enzyme which removes...Restriction Enzyme Cloning Restriction enzyme (endonuclease) based molecular cloning is the "classic" cloning...naturally produced by certain bacteria and archaea, cleave double stranded DNA (dsDNA) at specific sequence...cloning project. Restriction enzyme cloning also leaves behind a short scar in the DNA sequence and can...cloning utilizes the Taq polymerase which naturally leaves a single adenosine (A) overhang on the 3' end of...

  7. Plan Your Experiment

    Type
    Guide
    ...targets may not result in equivalent cleavage efficiency. In fact, cleavage efficiency may increase or decrease...are denatured-renatured, treated with a nuclease that cleaves DNA heteroduplexes, and run on an agarose...decrease expression of a target gene? Once you have a clear understanding of your experimental goal, you are...Infects dividing and non-dividing cells AAV is the least toxic method for in vivo viral delivery RNA delivery... sequence and target DNA may result in reduced cleavage. The number of alleles for each gene may vary ...likely to result in the most efficient on-target cleavage. The gRNA target sequence needs to match the target...gRNA design. In general, off-target sites are not cleaved as efficiently when mismatches occur near the PAM...

  8. Antibody Guide

    Type
    Guide
    ...code inserted into the plasmid. This produces the least amount of variation between antibodies, allows for... induced to express antibodies. Antibodies are released into the growth medium and can either be used ...fragments - Fab fragments are created by enzymatically cleaving the variable regions of an antibody (each Fab ...they should be stored at -20 °C in aliquots of at least 10 µL. Antibodies cannot tolerate freeze-thaw cycles...single cells. Includes: Flow cytometry Read on to learn more about the applications common to each category...should be run with your protein of interest and at least one ubiquitously expressed protein such as GAPDH...protein:DNA complexes. After capture, the DNA is released from the complex and can be sequenced and amplified...

  9. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Read our adeno-associated viral vector guide to learn about AAV vector components, production and common...recombination between the transfected plasmids could lead to the production of replication-competent viruses...element necessary for RABV trans-synaptic transport. Learn more about these and other initiatives in our blog... packaged in the relatively small rAAV capsids. Learn more about optogenetics by watching our Optogenetics...stimulates the expression of transgenes via increased nuclear export. Poly(A) signal in cis The polyadenylation...polyadenylation or poly(A) signal aids in the nuclear export of RNA and RNA translation, and promotes RNA transcript...like viruses or bacteria, within a host organism, leading to disease. Insertional mutagenesis Random insertion...

  10. New Antibody Animation!

    Type
    Blog Post
    ...Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what ...

  11. Lentiviral Vector Guide

    Type
    Guide
    ...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...lentiviruses has a long incubation period that eventually leads to serious disease and occurs in many species. Some...comparison of second- and third-generation systems, please refer to Table 1. Figure 4: Third-generation lentiviral...HEK293T cells will produce the viral vectors and release them into the supernatant. After an initial media...stimulates the expression of transgenes via increased nuclear export. RRE in cis Rev Response Element; he sequence...and partially spliced transcripts to facilitate nuclear export. Provided by a separate plasmid from gag...like viruses or bacteria, within a host organism, leading to disease. Lentivirus A retrovirus from the Retroviridae...

  12. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ... to take our word for it!  “When people want to learn about plasmids, they don’t go to Wikipedia; they...

  13. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    ...fundamental technical lab skills, like pipetting! We’ll release a new tip every two weeks during the school year...

  15. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ... "what is an IgE isotype?"  @addgene Ready to learn about antibodies? Our new antibodies animation has...

  18. CRISPR Guide Update

    Type
    Blog Post
    ...BioRender.com. Figure 1: PRIME editing Please check out the updated guide for a great introduction...
Showing: 821 - 840 of 1015 results