We narrowed to 902 results for: Nes

1. Plasmids 101: Yeast Vectors

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   Blog Post

   ...discussed how the regulation of bacterial ORIs determines plasmid copy number within the bacterial cell...

2. Will You Be My Mentor? Finding and Asking for Mentoring Support

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   Blog Post

   ...often have mentoring programs. The Healthcare Businesswomen’s Association hosts an excellent group mentoring...

3. Summer SciComm Series: Cognitive Load

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   Blog Post

   ... the slide deck (or reminding them to put their phones away), or maybe I was assuming something was intuitive...

4. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   Blog Post

   ...fuses MPST to roGFP2. Because roGFP2 contains two cysteines near the chromophore that are susceptible to redox...

5. Lighting Up Cell Signaling with Photoswitchable Kinases

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   Blog Post

   ...photoactivatable fluorescent proteins in empty backbones in the Michael Davidson Collection Find Photoswitchable...

6. Choosing the B(right)est Fluorescent Protein: Photostability

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   Blog Post

   ...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular...

7. Troubleshooting Your Plasmid Cloning Experiment

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   Blog Post

   ...cloning strategy. Once you've identified potential clones after the screening, sequence the plasmid. Many...

8. Visualizing Protein Turnover In Situ

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   Blog Post

   ... Professor Schuman’s lab showed that FUNCAT’s usefulness can be extended to determine the turnover of ...

9. RNA Extraction Without A Kit

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   Blog Post

   ...all-in-one acid-guanidinium-phenol solution that combines the homogenization solution and phenol addition...

10. Antibodies 101: Fab Fragments

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    Blog Post

    ...heavy chains. In the lab, this is the region that determines what secondary antibody you should use when performing...

11. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post

    ...Some of these synthetic modifications, specifically ones which do not contain a 5’ triphosphate in the gRNA...

12. Developing Lab Management Software for Biology

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    Blog Post

    ...how software can be structured to allow for the messiness of biology. These examples are intentionally trivial...

13. The Future of Research Symposium Boston 2015

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    Blog Post

    ...generating too many trainees per PI” “Lack of awareness about how the system operates and functions” “...

14. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post

    ...limit of roughly 0.1%. Thus, rare, pre-existing clones that are present at less than 0.1% of the total...

15. Plasmids 101: Cre-lox

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    Blog Post

    ...removal – in conventional mouse targeting, targeted clones are selected for using a resistance marker; however...

16. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ...exceptions to our standard workflow.  Table 1: Guidelines for the data fields in the deposit spreadsheet...

17. Mouse Modeling, Part 1: Genetically Engineered Mice

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    Blog Post

    ...scientists must genotype each animal to identify the ones with the desired genotype. Inducible and conditional...

18. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post

    ... via plasmids or by the creation of stable cell lines and these cells are then grown in biotin-containing...

19. Lab Automation at Addgene

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    Blog Post

    ...t electronic but it is one of the most helpful machines I have used in the lab. It’s especially useful...

20. High Complexity Golden Gate Assembly with NEB’s DAD

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    Blog Post

    ... 500 times the number of plaques than the linear ones. The 52-piece circular assembly of the T7 genome...