We narrowed to 910 results for: Nes

1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   ... alternative initiation, so they mutated the methionines at position 14 and 21 to create GEmCherry2. The...

2. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   Blog Post

   .... A phage encoding the deaminase TadA-7.10 A mutagenesis plasmid controlling the phage mutation rate ...

3. How Addgene Creates Content

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   Blog Post

   ... videos? In this post, we invite you behind the scenes in our content writing process, and to even get...

4. How to Make Friends and Meet People at a Scientific Conference

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   Blog Post

   ....  Before the conference Make sure you have a business card – even students and postdocs. There are many...

5. 3 Challenges in Plant Synthetic Biology

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   Blog Post

   ...Department at CSU. When not pursuing scientific or business enterprises, he can be found climbing mountains...

6. Plasmids 101: Yeast Vectors

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   Blog Post

   ...discussed how the regulation of bacterial ORIs determines plasmid copy number within the bacterial cell...

7. Will You Be My Mentor? Finding and Asking for Mentoring Support

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   Blog Post

   ...often have mentoring programs. The Healthcare Businesswomen’s Association hosts an excellent group mentoring...

8. Antibodies 101: Fab Fragments

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   Blog Post

   ...heavy chains. In the lab, this is the region that determines what secondary antibody you should use when performing...

9. Lighting Up Cell Signaling with Photoswitchable Kinases

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   Blog Post

   ...photoactivatable fluorescent proteins in empty backbones in the Michael Davidson Collection Find Photoswitchable...

10. Choosing the B(right)est Fluorescent Protein: Photostability

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    Blog Post

    ...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular...

11. Summer SciComm Series: Cognitive Load

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    Blog Post

    ... the slide deck (or reminding them to put their phones away), or maybe I was assuming something was intuitive...

12. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type

    Blog Post

    ...fuses MPST to roGFP2. Because roGFP2 contains two cysteines near the chromophore that are susceptible to redox...

13. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post

    ...cloning strategy. Once you've identified potential clones after the screening, sequence the plasmid. Many...

14. RNA Extraction Without A Kit

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    Blog Post

    ...all-in-one acid-guanidinium-phenol solution that combines the homogenization solution and phenol addition...

15. Visualizing Protein Turnover In Situ

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    Blog Post

    ... Professor Schuman’s lab showed that FUNCAT’s usefulness can be extended to determine the turnover of ...

16. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post

    ...Some of these synthetic modifications, specifically ones which do not contain a 5’ triphosphate in the gRNA...

17. Developing Lab Management Software for Biology

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    Blog Post

    ...how software can be structured to allow for the messiness of biology. These examples are intentionally trivial...

18. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    ...generating too many trainees per PI” “Lack of awareness about how the system operates and functions” “...

19. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    ...limit of roughly 0.1%. Thus, rare, pre-existing clones that are present at less than 0.1% of the total...

20. Plasmids 101: Cre-lox

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    Blog Post

    ...removal – in conventional mouse targeting, targeted clones are selected for using a resistance marker; however...