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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,018 results for: PLE;

Showing: 821 - 840 of 1018 results

  1. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...food pantry or community arts organization. Many people are surprised to learn that Addgene is officially...thought leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters...

  2. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...promoter lead to higher transcriptional activation. Multiple nuclear localization signals (NLS) increase protein... However, this system is far from perfect. For example, If your fusion protein is expressed at low levels...

  3. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ... are leaders in the field of microbiology with multiple high-profile publications. My classmates consisted...portion of the paper will be generated. After you complete a publication quality figure, produce the figure...

  4. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...organoid model (Sun, et al. 2021).  Figure 1: Example schematic from the Organoid EasyTag system, showing...Resources page. Here are some highlights from the past couple months: A new lentiviral CRISPR library from Alec...

  5. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...this frequency can indicate behavioral state, for example, if someone is awake, agitated, or asleep. Though...neurotransmitters in various species and while exhibiting complex behaviors. Find dopamine and other biosensors in...

  6. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...article is published. This policy gives us time to complete necessary quality control and legal approvals ... your trust, and meet the community’s needs, so please let us know what you think. We appreciate your ...

  8. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...be successful in a specific role or cycle. For example, if you try to lead when you are expected to be...Got You Here Won't Get You There: How Successful People Become Even More Successful, by Marshall Goldsmith...

  9. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...has the CMV promoter for mammalian expression, a multiple cloning site to insert YGOI, a bacterial selection...e.g. pLKO.1 - TRC cloning vector). Check out our complete guide to empty backbones for additional details...

  11. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...the public’s perception of head injury risk. In completing a master’s thesis in science communication, I... Department of Science Communication where he completed a master's degree in 2020, his research explores...

  12. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...(on a schedule) I hope you all know by now that people get jobs at least 85% of the time because of relationships...pretty easy to make 30 minute appointments with people all over the world. Think about contacting alumni...

  15. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...The technique relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize...PCR product. If you need to decrease error rate, please use one of these methods to ensure your insert ...

  16. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...efficient in primary fibroblasts. iTOP also works in multiple types of primary mouse cells, including neuronal...and continued protein expression would require multiple transduction events. For these reasons, iTOP is...

  17. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... out more than necessary for my interview. The simplest place I could think of to start was “Marketing...about marketing would be to start reading a broad simple overview, learn some jargon and key phrases and...

  18. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...and transgene expression (Zhou et al. 2017). For example, mutations in the RBE site reduce binding with ...AAV2 ITRs can be cross-packaged with capsids from multiple AAV serotypes (Wilmott et al., 2019), and different...

  19. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...point of reference, Addgene commonly prepares NGS samples using both pre- and post-amplification DNA and ...representation is most easily understood visually. As an example, the curves from our recent amplification of the...

  20. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...(by CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating...Central PMCID: PMC4364232. Ma, Hanhui et al. “Multiplexed labeling of genomic loci with dCas9 and engineered...
Showing: 821 - 840 of 1018 results