We narrowed to 1,045 results for: STI

...process known as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that...driving particular cells or responses. And there are still antibody isotypes and Fc receptors that we don’t...

...sometimes even genetic pathways that are worth investigating for drug development. Due to the difficulty ... to determine if a patient would respond to an existing therapy. In theory, CRISPR could even be used ...

...neutralize the nuclease activity. For strains that still carry this gene, there are usually extra steps like...the pH preparation stabilizing the plasmid, and still allowing most downstream processing. On the other...

...two gRNAs.  For nickase applications, a common question is: how should the gRNAs be oriented in comparison...insertions. These profiles are likely due to the distinct overhang patterns generated by the nickases; D10A...

... we can predict the resulting band sizes when digesting the plasmid sequence with one or more enzymes....Reference Pages Basic Molecular Biology Protocols Diagnostic Restriction Digest Protocol ...

...for new ways to serve the research community. Questions? Feel free to reach out to us on Twitter, Facebook... Ishrat, Jane, Jason, Jessica, Joel, Jonathan, Justine, Karen, Kate, Kayla, Kelly, Kory, Kris, Leila, ...

...I just keep learning on the job. It keeps it interesting.” And while his official title may be science... his boss was looking for someone to take over hosting them. Since then, webinars make up a larger part...

...has “literally enabled sharing with 100's of investigators worldwide. Now everything from every paper is... Addgene is that we cover all of the shipping logistics and facilitate all material transfer agreements...

... my kids like any other youngster. His parents, Kristi and Rico, had to keep him from running too much...the fight against FOP. Second, Addgene will be hosting the AJ’s Boston League team (Contributing many ...

...CRISPR screens symposia outlined the fantastic work this Broad Institute lab is doing creating well-optimized...

... I've been answering biology customer service questions for nearly 7 years, and my colleagues and I have... worked so hard to make this poster, offered suggestions and tips, and helped get me to this meeting. ...

...plasmid in addition to its own chromosomal DNA, costing additional resources to maintain the plasmid. Adding...cell division (bacteriostatic); however, the distinction between the two categories may be a bit of a ...

...because the secondary antibody is not able to distinguish between the primary antibody and endogenous IgGs...your samples, but know that all is not lost if you still see non-specific staining! Hopefully, these solutions...

... viral vectors, which are used in the lab, are distinct from viruses — we’ll get to that in a bit! Viral...bud out, instead of lysing, but they do generally still kill the infected cell.) This is in contrast to ...

...the fetal tissue which HEKs were isolated from is still a mystery, but it’s currently assumed that the cells...native channels.  No matter what your research question, HEK-293 is known for reproducible results. It...

... strong a signal as with the wild-type AraC, it still reached the level of expression needed for most ...bacteriograph was created using 160 individual images stitched together. Image from Romano et al., 2021 with ...

...have recently received a grant from the National Institutes of Health that will address this problem.The ... represent the official views of the National Institutes of Health.     References and Resources References...

...world mobilized on COVID-19 research, cures, and diagnostics. Almost immediately we began to receive urgent...Addgene plays in accelerating research. Yet this was still not enough to ensure we could keep up with demand...

...designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP variant...molecule-fluorescence assay to monitor restriction enzyme digestion of single molecules in real-time. Find plasmids...

... show that this basic concept of gRNA design is still challenging for researchers using CRISPR, and that...you’d like to avoid editing the genome and just stick to RNA? Cas13 may be able to help you do that! How...