We narrowed to 1,045 results for: STI
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TypeBlog Post...two gRNAs. For nickase applications, a common question is: how should the gRNAs be oriented in comparison...insertions. These profiles are likely due to the distinct overhang patterns generated by the nickases; D10A...
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Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog Post... we can predict the resulting band sizes when digesting the plasmid sequence with one or more enzymes....Reference Pages Basic Molecular Biology Protocols Diagnostic Restriction Digest Protocol ... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog Post...for new ways to serve the research community. Questions? Feel free to reach out to us on Twitter, Facebook... Ishrat, Jane, Jason, Jessica, Joel, Jonathan, Justine, Karen, Kate, Kayla, Kelly, Kory, Kris, Leila, ... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog Post...has “literally enabled sharing with 100's of investigators worldwide. Now everything from every paper is... Addgene is that we cover all of the shipping logistics and facilitate all material transfer agreements... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog Post...neutralize the nuclease activity. For strains that still carry this gene, there are usually extra steps like...the pH preparation stabilizing the plasmid, and still allowing most downstream processing. On the other... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog Post...analysis, Tm and primer dimer estimation, and other primer characteristics. NEB Data-optimized Assembly... -
Antibodies 101: Fc Effector Functions
TypeBlog Post...process known as phagocytosis. Other functions We’re still learning about Fc effector functions and all that...driving particular cells or responses. And there are still antibody isotypes and Fc receptors that we don’t... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog Post...sometimes even genetic pathways that are worth investigating for drug development. Due to the difficulty ... to determine if a patient would respond to an existing therapy. In theory, CRISPR could even be used ... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...I just keep learning on the job. It keeps it interesting.” And while his official title may be science... his boss was looking for someone to take over hosting them. Since then, webinars make up a larger part... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post... my kids like any other youngster. His parents, Kristi and Rico, had to keep him from running too much...the fight against FOP. Second, Addgene will be hosting the AJ’s Boston League team (Contributing many ... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post...CRISPR screens symposia outlined the fantastic work this Broad Institute lab is doing creating well-optimized... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog Post... I've been answering biology customer service questions for nearly 7 years, and my colleagues and I have... worked so hard to make this poster, offered suggestions and tips, and helped get me to this meeting. ... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...plasmid in addition to its own chromosomal DNA, costing additional resources to maintain the plasmid. Adding...cell division (bacteriostatic); however, the distinction between the two categories may be a bit of a ... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...function is inactivated by the separation of its synergistic domains. This paper demonstrated that the migration...mediated Editing (ACE) in real-time. The reporter constitutively expresses eGFP to indicate reporter abundance... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post... show that this basic concept of gRNA design is still challenging for researchers using CRISPR, and that...you’d like to avoid editing the genome and just stick to RNA? Cas13 may be able to help you do that! How... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog Post...Backstrom, a biochemist in the Vanderbilt Eye Institute and Tonia Rex's lab. A common strategy to determine...Backstrom, Ph.D is a biochemist in the Vanderbilt Eye Institute and is particularly interested in cell signaling... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post...But what makes software good? Fortunately, that question has been thoroughly answered by experts in human-computer...engaging with users of the software. We welcome suggestions about common features from anyone who uses SnapGene... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...world mobilized on COVID-19 research, cures, and diagnostics. Almost immediately we began to receive urgent...Addgene plays in accelerating research. Yet this was still not enough to ensure we could keep up with demand... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP variant...molecule-fluorescence assay to monitor restriction enzyme digestion of single molecules in real-time. Find plasmids... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post... strong a signal as with the wild-type AraC, it still reached the level of expression needed for most ...bacteriograph was created using 160 individual images stitched together. Image from Romano et al., 2021 with ...