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We narrowed to 1,049 results for: URE

Showing: 821 - 840 of 1049 results
  1. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...and S. cerevisiae (Tuller et. al., 2010). Figure 1: An overview of peptide synthesis. A ribosome...as Hoogsten base pairs and wobble pairs.  Figure 2: (A) The codon wheel depicts the 64 codons that...PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon usage bias, and...
  2. Tips for CRISPR Gene Editing in Mice

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    Blog Post
    ...Addgene. In vitro screening for the best gRNA Figure 1: Validation of double strand break (DSB) mediated...were fusing, you may have several mosaic mice. To figure out what alleles are present in these mice, you...favourite mammalian model organisms in the near future.  Many thanks to our guest blogger Samantha Young...
  3. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    Blog Post
    ...than the other (Sandomenico et al., 2020). Figure 1: Comparison between the IgG antibody and scFv...of panning and scFv characterization.    Figure 2: Phage display starts with a pooled library of...Bates A, Power CA (2019) David vs. Goliath: The Structure, Function, and Clinical Prospects of Antibody ...
  4. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post
    ... dsDNA breaks: following a cut, the error-prone nature of non-homologous end joining (NHEJ) often leads...database, which relies on CAGE-seq to directly capture the mRNA cap, provides the most accurate mapping...examined the ability to use sequence-based and other features to nominate gRNAs that are likely to be active...
  5. CRISPR Guide Update

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    Blog Post
    ...accompanied by new or refreshed figures made using BioRender.com. Figure 1: PRIME editing Please...
  6. Addgene is Stopping Use of X (formerly Twitter)

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    Blog Post
    ... significantly away from the functionality and features that previously made the platform a mainstay of...not delete @addgene on X. This will additionally ensure that the @addgene handle is always associated with...
  7. The 12 Days of CRISPR: 2021

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    Blog Post
    ...the first day of CRISPR, Addgene gave to me: a miniature CRISPR-Cas system for mammalian use. On the second...Addgene gave to me: programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR...
  8. Lucky Thirteen: Guest Contributors of 2023

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    Blog Post
    ...sharing all the incredible guest posts and stories featured in our blog in 2023. Some of these were written...Course-Based Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew Acoustic Reporter Genes...
  9. New FLIS Plasmid (April Fools 2022)

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    Blog Post
    ...interrogate many scientific questions. But these tiny creatures do tend to come with a rather astonishing ability...system for locating and containing escaped flies. Nature 4:1-22. https://doi.org/4.01.2022/hafd...
  10. 2021 Deck the Lab Winners!

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    Blog Post
    ... by Xi Chen (@seleena78)   Honorable Mention: Culture Warrior The Moore lab, who was full of holiday ...honorable mention for their second submission.    Our Cultured Warrior (made by Nate using pipette boxes, wire...
  11. Find and Share AAV Data with Addgene's New AAV Data Hub

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    Blog Post
    ..., you will be able to search for your favorite features, such as serotype, promoter, or tool type. Drilling...accepting data for materials distributed by Addgene.  Future directions As the AAV Data Hub grows, we hope to...
  12. Top Requested Plasmid of 2017 - pMD2.G

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    Blog Post
    ...guide page. For more protocols and information, be sure to check out the Trono Lab website. If you have ... plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours...
  13. Deck the Lab 2022!

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    Blog Post
    ...performing routine lab maintenance tasks. Hot tip: make sure your PI/facilities are okay with the decorations...@seleena78)   Here’s how to enter: Post your picture or video for either contest on Twitter or Instagram...
  14. Our New Antibody Mascot is....

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    Blog Post
    ...  Fig. 1: Meet...well, frankly, we're not sure yet!   This as-yet–unnamed friend is a huge ...mascot, an antibody joining us on our scientific adventures.  You can submit your name suggestions here or...
  15. Depositor Week 2020 is Happening Now. Join us!

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    Blog Post
    ...Fun stats and trivia begins! Tuesday: Depositor features (plasmid deposits and blog posts showcasing the...year in recent years. Despite COVID-19 and lab closures, we’re expecting to reach ~14,000 plasmids deposited...
  16. Purple Plants that Report Pollutants

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    Blog Post
    ...The authors call these “sentinel plants.” Figure 1: Dr. Malia Gehan, Dr. Dong-Yeon Lee, and Dr. ...imaging. Step aside, green thumbs. Perhaps in the future we’ll be discussing who has a purple thumb! References...
Showing: 821 - 840 of 1049 results